1. Association between interleukin‑36γ and tumor progression in non‑small cell lung cancer
- Author
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Honghong He, Yuehua Feng, Yibei Zhu, Yanzheng Gu, Dan Xu, Liyan Shi, Lin Liu, Huijun Zhou, and Jian Wang
- Subjects
squamous cell carcinoma ,0301 basic medicine ,Cancer Research ,medicine.medical_treatment ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Lung cancer ,non-small cell lung cancer ,IL-36γ ,Tissue microarray ,Oncogene ,business.industry ,Cancer ,Articles ,Immunotherapy ,Cell cycle ,lung adenocarcinoma ,medicine.disease ,respiratory tract diseases ,030104 developmental biology ,Oncology ,Tumor progression ,030220 oncology & carcinogenesis ,immunohistochemistry ,Cancer research ,Adenocarcinoma ,business - Abstract
Immunotherapy is effective in improving the survival and prognosis of patients with non-small cell lung cancer (NSCLC), and identifying effective immunomarkers is important for immunotherapy. Interleukin (IL)-36γ is a novel immunomarker that has an important function in the antitumor immune response. The present study investigated the association between IL-36γ and NSCLC to provide novel insight into immunotherapy for patients with NSCLC. Tissue microarrays of lung adenocarcinoma and squamous cell carcinoma were purchased for immunohistochemical analysis of IL-36γ expression levels and clinical parameters. In addition, fresh clinical NSCLC and adjacent normal tissue samples were collected to analyze IL-36γ mRNA expression levels using quantitative PCR. IL-36γ protein was primarily located in the cytoplasm, with a small quantity in the nucleus, and IL-36γ mRNA and protein expression levels in lung cancer tissues were significantly higher compared with those in adjacent normal tissues. Elevated IL-36γ protein expression levels were significantly associated with a higher tumor grade of lung adenocarcinoma; however, IL-36γ mRNA expression levels were inversely associated with the clinical Tumor-Node-Metastasis stage in patients with lung squamous cell carcinoma. In addition, patients with adenocarcinoma with high IL-36γ protein expression levels tended to longer post-operative survival times. These findings indicate that IL-36γ may have potential as an immunomarker for prediction of tumor progression and survival in patients with NSCLC.
- Published
- 2020