1. JNK pathway inhibition enhances chemotherapeutic sensitivity to Adriamycin in nasopharyngeal carcinoma cells
- Author
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Ming Zhao, Li Xiang, Jinhui Xu, Linglin Yang, Kang Xiong, Jingbo Wu, Juan Fan, Yanling Zhang, Yun Lu, Qinglian Wen, Yong Liu, Jianwen Zhang, Jing Feng, ShaoZhi Fu, and Bi-Qiong Wang
- Subjects
0301 basic medicine ,Cancer Research ,Cell type ,Cell growth ,Kinase ,Cell ,Articles ,Cell cycle ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Nasopharyngeal carcinoma ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,polycyclic compounds ,Viability assay - Abstract
The role of c-Jun N-terminal kinases (JNKs) in the pathogenesis of cancer is well-known due to their involvement in carcinogenesis. Although previous studies have discussed different functions of JNKs depending on cell type, the present study aimed to investigate the function of JNKs in nasopharyngeal carcinoma (NPC) cells, as well as their involvement in chemotherapy sensitivity to Adriamycin. The present results showed that Adriamycin administration reduced cell viability and led to elevated expressions of c-Jun, phosphorylated JNK and phosphorylated c-Jun, indicating an activated JNK pathway. Notably, JNK inhibition by SP600125 also reduced cell growth. Thus, Adriamycin treatment combined with SP600125 was more effective on cell growth inhibition than each agent alone. The apoptosis analysis confirmed the reduction in cell growth. Therefore, these data provide evidence that the JNK pathway activity is negatively associated with cell viability, and its decline could sensitize NPC cells to Adriamycin.
- Published
- 2015