1. Effect of vascular endothelial growth factor-C expression on lymph node metastasis in human cholangiocarcinoma
- Author
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Zhao Liu, Jiayong Wang, Chang Yuan, Sen Guo, Yanfeng Liu, Jian Yu, and Rui Zhao
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,Oncogene ,medicine.diagnostic_test ,business.industry ,Cancer ,Cell migration ,Articles ,Cell cycle ,medicine.disease ,digestive system ,digestive system diseases ,Metastasis ,Oncology ,Vascular endothelial growth factor C ,Biopsy ,Medicine ,Immunohistochemistry ,business ,neoplasms - Abstract
The present study aimed to evaluate the expression of vascular endothelial growth factor C (VEGF-C) in human cholangiocarcinoma tissues and its role in metastasis in vitro. A total of 65 biopsy samples of cholangiocarcinoma, plus the FRH-0201 cell line, were investigated. The expression of VEGF-C in the human cholangiocarcinoma specimens was evaluated by immunohistochemistry (IHC). The effect of VEGF-C on tumor cell migration and proliferation was measured by MTT and Transwell assays in the FRH-0201 cell line. According to the IHC results, the biopsies of human cholangiocarcinoma were stained positively for VEGF-C, with a positive rate of 75.4% (49/65). Moreover, VEGF-C was expressed at a higher level in the patients with lymph node metastasis than in those without lymph node metastasis. In vitro, VEGF-C exhibited marked growth stimulation below the concentration of 5 ng/ml and was able to promote cholangiocarcinoma cell migration significantly. These findings suggested that VEGF-C may be a useful factor to predict lymph node metastasis in cholangiocarcinoma tissues and indicates that VEGF-C plays a significant role in proliferation and migration in cholangiocarcinoma cells.
- Published
- 2014