1. Role of microRNA-4458 in patients with non-small-cell lung cancer
- Author
-
Yuehong Wang, Lidao Bao, Guomin Wei, Linlin Wang, Gerile Wuyun, and Agula Bo
- Subjects
0301 basic medicine ,A549 cell ,Cancer Research ,Oncogene ,Cancer ,Articles ,Cell cycle ,Biology ,medicine.disease_cause ,medicine.disease ,respiratory tract diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Cyclin D1 ,Oncology ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Carcinogenesis ,Lung cancer ,Survival rate - Abstract
Incidence and progression of non-small-cell lung cancer (NSCLC) is a multi-factor, multi-step process. The present study investigated the association between the expression level of microRNA (miR)-4458 in NSCLC and paracarcinoma liver tissues and survival rates, and studied the biological functions of miR-4458 at the cellular and protein level. NSCLC and paracarcinoma tissues were sequenced using a miR expression chip. The association between miR-4458 expression and tumor-node-metastasis staging, total survival rate and relapse-free survival rate was analyzed. miR-4458 was subjected to target gene prediction. The target protein of cyclin D1 (CCND1) was verified with western blot analysis, immunohistochemistry and a luciferase reporter assay. The relative level of miR-4458 in paracarcinoma tissues of 9 NSCLC patients decreased from 2.38 to 0.65 (P
- Published
- 2016