1. [Corrigendum] Activation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620
- Author
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Wenjin Zhang, Yitao Jia, Jingbao Wang, Suqiao Zhang, Zhongxin Li, Shunmao Ma, Zhihui Duan, Lingling Miao, Zujian Jin, Bin Gu, and Zhaolong Zhao
- Subjects
Receptors, CXCR4 ,Cancer Research ,Pathology ,medicine.medical_specialty ,Cell ,epithelial-mesenchymal transition ,Vimentin ,colorectal cancer ,Flow cytometry ,Transforming Growth Factor beta1 ,Cell Movement ,Cell Line, Tumor ,medicine ,Humans ,Receptor, PAR-1 ,Platelet ,CXC chemokine receptors ,Platelet activation ,Epithelial–mesenchymal transition ,chemotaxis ,platelet ,Oncogene ,biology ,medicine.diagnostic_test ,transforming growth factor β1 ,Chemistry ,protease activated receptor-1 ,Cancer ,Chemotaxis ,General Medicine ,Articles ,Cell cycle ,medicine.disease ,Cadherins ,Platelet Activation ,Molecular medicine ,Molecular biology ,MicroRNAs ,Protease-Activated Receptor 1 ,medicine.anatomical_structure ,Oncology ,Cell culture ,Colonic Neoplasms ,Cancer research ,biology.protein ,Corrigendum ,Oligopeptides - Abstract
The aim of the present study was to examine the role of protease-activated receptor-1 (PAR1)-stimulated platelet activation in the epithelial-mesenchymal transition (EMT) and migration of colon cancer cells, and to identify the underlying mechanisms. TFLLR-NH2, a PAR1 agonist, was used to activate platelets and the platelet supernatants were used to treat the SW620 colon cancer cell line. Expression of E-cadherin and vimentin on SW620 cells was detected by immunofluorescence and western blotting, and the level of the transforming growth factor β1 (TGF-β1) was measured using ELISA following the activation of platelets by TFLLR-NH2. miR-200b expression was detected using quantitative PCR in SW620 cells. In order to investigate the chemotactic ability of the SW620 cells, the expression of CXC chemokine receptor type 4 (CXCR4) was measured by flow cytometry. Transwell migration assays were performed following exposure of the cells to the supernatant of PAR1-activated platelets. SW620 cells cultured in the supernatant of TFLLR-NH2-activated platelets upregulated E-cadherin expression and downregulated the vimentin expression. In the in vitro platelet culture system, a TFLLR-NH2 dose-dependent increase of secreted TGF-β1 was detected in the supernatant. The activation of PAR1 on the platelets led to the inhibition of miR-200b expression in the SW620 cells that were cultured in platelet-conditioned media. The number of SW620 cells that penetrated through the Transwell membrane increased with the dose of TFLLR-NH2 used to treat the platelets. The percentage of CXCR4-positive SW620 cells was significantly higher when they were exposed to the supernatant of platelets cultured for 24 h with PAR1 agonist than when cultured in non-conditioned media (40.89 ± 6.74 vs. 3.47 ± 1.40%, P < 0.01). Platelet activation with a PAR1 agonist triggered TGF-β secretion, which induced EMT of SW620 human colon cancer cells via the downregulation of miR-200b expression, and activated platelets had a chemotactic effect on colon cancer cells mediated by the upregulation of CXCR4 on the cell surface.
- Published
- 2014