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// Xiaoyan Wang 1, 2 , Guanghua Chen 1, 2 , Chao Huang 1 , Hualei Tu 3 , Jilong Zou 4 and Jinglong Yan 1 1 Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, P.R. China 2 Key Laboratory of the Ministry of Education, Ministry of Myocardial Ischemia, Harbin 150086, P.R. China 3 Burn Department, The Fifth Hospital of Harbin, Harbin 150086, P.R. China 4 Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, P.R. China Correspondence to: Jinglong Yan, email: yanjinglong246@163.com Keywords: extracellular matrix, BMSCs, tissue engineering, scaffold, bone regeneration Received: August 24, 2017 Accepted: September 20, 2017 Published: October 09, 2017 ABSTRACT The extracellular matrix(ECM), which is primarily composed of collagens and proteoglycans, plays a key role in cell proliferation, differentiation, and migration and interactions between cells. In this study, we produced chitosan/gelatin/bone marrow stem cells-derived extracellular matrix(C/G/BMSCs-dECM) scaffolds via lyophilization and cross-linking, and chitosan/gelatin(C/G) scaffolds were used as controls. For the C/G/BMSCs-dECM scaffolds, the average pore size was 289.17 ± 80.28 μm; the average porosity was 89.25 ± 3.75%; the average compressive modulus was 0.82 ± 0.07 MPa; and the average water uptake ratio was 13.81 ± 1.00. In vitro , the C/G/BMSCs-dECM scaffolds promoted bone marrow stem cells(BMSCs) attachment and proliferation. Moreover, improved osteogenic differentiation was observed for these scaffolds. Thus, C/G/BMSCs-dECM is a promising material for bone tissue engineering.