1. microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma
- Author
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Cao, Ke, Li, Jingjing, Chen, Jia, Qian, Li, Wang, Aijun, Chen, Xiang, Xiong, Wei, Tang, Jintian, Tang, Shijie, Chen, Yong, Chen, Yao, Cheng, Yan, and Zhou, Jianda
- Subjects
Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Biotechnology ,Cancer ,microRNA ,radiation ,glucose ,HIF-1a ,lactate dehydrogenase A ,HIF-1α ,Oncology and carcinogenesis - Abstract
Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR-33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma.
- Published
- 2017