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Your search keyword '"Marshall, Steven H."' showing total 10 results
Start Over Author "Marshall, Steven H." Journal open forum infectious diseases
10 results on '"Marshall, Steven H."'

1. Characterization of a Novel Pathogen in Immunocompromised Patients:Elizabethkingia anophelis—Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors

Author

Yasmin, Mohamad, primary, Rojas, Laura J, additional, Marshall, Steven H, additional, Hujer, Andrea M, additional, Cmolik, Anna, additional, Marshall, Emma, additional, Boucher, Helen W, additional, Vila, Alejandro J, additional, Soldevila, Maxime, additional, Diene, Seydina M, additional, Rolain, Jean-Marc, additional, and Bonomo, Robert A, additional

Published
2023
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2. Characterization of a Novel Pathogen in Immunocompromised Patients: Elizabethkingia anophelis—Exploring the Scope of Resistance to Contemporary Antimicrobial Agents and β-lactamase Inhibitors.

Author

Yasmin, Mohamad, Rojas, Laura J, Marshall, Steven H, Hujer, Andrea M, Cmolik, Anna, Marshall, Emma, Boucher, Helen W, Vila, Alejandro J, Soldevila, Maxime, Diene, Seydina M, Rolain, Jean-Marc, and Bonomo, Robert A

Abstract

Background Elizabethkingia anophelis is an emerging Gram-negative nonlactose fermenter in the health care setting, where it causes life-threatening infections in immunocompromised patients. We aimed to characterize the molecular mechanisms of antimicrobial resistance and evaluate the utility of contemporary antibiotics with the intent to offer targeted therapy against an uncommonly encountered pathogen. Methods Whole-genome sequencing (WGS) was conducted to accurately identify isolate species and elucidate the determinants of β-lactam resistance. Antimicrobial susceptibility testing was performed using broth microdilution and disk diffusion assays. To assess the functional contribution of the major metallo-β-lactamase (MBL) encoding genes to the resistance profile, bla BlaB was cloned into pBCSK(-) phagemid vector and transformed into Escherichia coli DH10B. Results WGS identified the organism as E. anophelis. MBL genes bla BlaB-1 and bla GOB-26 were identified, in addition to bla CME-2, which encodes for an extended-spectrum β-lactamase (ESBL). Plasmids were not detected. The isolate was nonsusceptible to all commonly available β-lactams, carbapenems, newer β-lactam β-lactamase inhibitor combinations, and to the combination of aztreonam (ATM) with ceftazidime-avibactam (CAZ-AVI). Susceptibility to the novel siderophore cephalosporin cefiderocol was determined. A BlaB-1 transformant E. coli DH10B isolate was obtained and demonstrated increased minimum inhibitory concentrations to cephalosporins, carbapenems, and CAZ-AVI, but not ATM. Conclusions Using WGS, we accurately identified and characterized an extensively drug-resistant E. anophelis in an immunocompromised patient. Rapid evaluation of the genetic background can guide accurate susceptibility testing to better inform antimicrobial therapy selection. [ABSTRACT FROM AUTHOR]

Published
2023
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3. 594. A Multi-Centered Study of the Clinical and Molecular Epidemiology of AmpC Cephalosporinase-Producing (AmpC) Enterobacteriaceae (Ent) Infections in Children

Author

Medernach, Rachel L, primary, Domitrovic, T Nicholas, additional, Hayani, Karen C, additional, Hujer, Andrea M, additional, Qureshi, Nadia K, additional, Marshall, Steven H, additional, Nguyen, David C, additional, Rudin, Susan D, additional, Rispens, Jared R, additional, Zheng, Xiaotian, additional, Weinstein, Robert A, additional, Bonomo, Robert A, additional, and Logan, Latania K, additional

Published
2019
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4. 2560. Multispecies Outbreak of KPC-2 Producing Enterobacteriaceae in a Chilean Pediatric Hospital

Author

Rojas, Laura J, primary, Marshall, Steven H, additional, Rivas, Lina M, additional, Spencer, Maria, additional, Rutter, Joseph, additional, Jacobs, Michael R, additional, Perez, Federico, additional, Coria, Paulina, additional, Valdivieso, Francisca, additional, Bralic, Rafael Araos, additional, Munita, Jose M, additional, and Bonomo, Robert A, additional

Published
2018
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6. 2395. Mechanism-Based-Susceptibility Testing (MBST) Using Disc Diffusion Assays (DDA) to Guide Treatment of Multidrug- and Extensively Drug-Resistant Pseudomonas aeruginosa (MDR-XDR-Pa) in a Cystic Fibrosis (CF) Lung Transplant Recipient; Are We Ready for Combination Therapy vs. MDR-XDR-Pa?

Author

Abbo, Lilian M, primary, Yasmin, Mohamad, additional, Marshall, Steven H, additional, Perez, Federico, additional, Corzo-Pedrosa, Mónica, additional, Camargo, Jose F, additional, Simkins, Jacques, additional, Aragon, Laura, additional, Anjan, Shweta, additional, Morris, Michele I, additional, Brozzi, Nicolas, additional, Loebe, Mathias, additional, Fulmer, Jesse, additional, Sinha, Neeraj, additional, Martinez, Octavio, additional, Perez-Cardona, Armando, additional, Colin, Andrew, additional, Cloke, Christina, additional, and Bonomo, Robert A, additional

Published
2018
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7. 2336. Resistance Mechanisms and Factors Associated With CTX-M-9 Group Extended-Spectrum β-Lactamase (ESBL)-Producing Enterobacteriaceae Infections in Children

Author

Medernach, Rachel L, primary, Domitrovic, T Nicholas, additional, Rispens, Jared R, additional, Hujer, Andrea M, additional, Qureshi, Nadia K, additional, Marshall, Steven H, additional, Nguyen, David C, additional, Rudin, Susan D, additional, Zheng, Xiaotian, additional, Weinstein, Robert A, additional, Bonomo, Robert A, additional, and Logan, Latania K, additional

Published
2018
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8. A Multicenter Study of the Clinical and Molecular Epidemiology of TEM- and SHV-type Extended- Spectrum β-Lactamase producing (ESBL) Enterobacteriaceae (Ent) Infections in Children

Author

Rispens, Jared R, primary, Medernach, Rachel L, additional, Hujer, Andrea M, additional, Domitrovic, T Nicholas, additional, Marshall, Steven H, additional, Rudin, Susan D, additional, Zheng, Xiaotian, additional, Qureshi, Nadia K, additional, Hayden, Mary K, additional, Weinstein, Robert A, additional, Bonomo, Robert A, additional, and Logan, Latania K, additional

Published
2017
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10. Emergence of Resistance to Colistin During the Treatment of Bloodstream Infection Caused by Klebsiella pneumoniae Carbapenemase–Producing Klebsiella pneumoniae.

Author

Kanwar, Anubhav, Marshall, Steven H, Perez, Federico, Tomas, Myreen, Jacobs, Michael R, Hujer, Andrea M, Domitrovic, T Nicholas, Rudin, Susan D, Rojas, Laura J, and Kreiswirth, Barry N

Abstract

We report the emergence of colistin resistance in Klebsiella pneumoniae carbapenemase (KPC)–producing Klebsiella pneumoniae after 8 days of colistin-based therapy, resulting in relapse of bloodstream infection and death. Disruption of the mgrB gene by insertion of a mobile genetic element was found to be the mechanism, which was replicated in vitro after exposure to subinhibitory concentrations of colistin and meropenem. [ABSTRACT FROM AUTHOR]

Published
2018
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