Search

Background: Gingival Overgrowth (GO) is a well documented and unwanted side effect that occurs mainly as a result of certain antiseizure, phenytoin. The aim of this study was to compare the effect of phenytoin on proliferation and production of IL1β and PGE2 in cultured human gingival fibroblasts (HGF) of children and adults. Materials and Methods: Normal HGFs were obtained from 4 healthy children and 4 adult and then were cultured with phenytoin (20 mg/ml). MTT test was used to evaluate the proliferation and ELISA to determine the level of IL1β and PGE2 production by HGFs. Analysis of proliferation were assessed by Independent T-Test and ANOVA analysis was used to assess the level of IL1β and PGE2 production with an a error level less than 0.05. Results: The proliferation of HGF was not affected significantly by phenytoin in both cultured fibroblast sources (P > 0.05). Phenytoin induced a significantly higher formation of IL1β and PGE2 in child’s HGFs as compared to adult’s HGFs (P < 0.05). Conclusion: The results suggest that different inflammatory responses and cytokine formation by child’s and adult’s HGFs are the probable key elements that cause different reactions of phenytoin therapy. More advanced and systematic studies are needed to verify these findings.

The original online version of this article (Vahabi, S., Moslemi, M., Nazemisalman, B. and Yadegari, Z. (2014) Phenytoin Effects on Proliferation and Induction of IL1β and PGE2 in Pediatric and Adults’ Gingival Fibroblasts. Open Journal of Stomatology, 4, 452-462. http://dx.doi.org/10.4236/ojst.2014.49061) was published without one of the coauthor mistakenly. To reflect the contribution and responsibility of the fifth author as well as the affiliations of the authors at the time of the study, we revised authorship and author affiliations of the article. The author wishes to correct the errors to: Surena Vahabi1, Masomeh Moslemi2, Bahareh Nazemisalman3*, Zahra Yadegari4, Faranak Mahjour5 1Periodontics Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2Pedodontic Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3Pedodontic Department, Dental School, ZUMS, Zanjan, Iran 4Biotechnology, Shahid Beheshti Medical University, Tehran, Iran 5Dental Research Center, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran