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• The radiation induced static mode degradation (SMD) was discovered and studied. • The threshold of SMD could be related with the composition of active fiber and increases with the increase of Ce. • When the Ce concentration increased by 35.5%, SMD threshold rose by 16% at most. We report on an experimental investigation of radiation induced static mode degradation in Yb-Ce co-doped pulsed fiber amplifiers. For investigating the impact of irradiation on beam quality, radiation-darkened fibers were irradiated by gamma rays. The laser properties of three types of fibers were investigated. With the pump power increased, static mode degradation appeared: M2 factor raised significantly and the output beam profile transformed from LP 01 into LP 11 shape without high-frequency component. The threshold of static mode degradation decreased with irradiation dose, and related to Yb-Ce co-doped fiber compositions. The doping of Ce benefits to the improvement of the static mode degradation threshold: in the case of the Ce concentration increasing by 35.5%, SMD threshold rose by 16% at most. [ABSTRACT FROM AUTHOR]