1. Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy
- Author
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Kuo Chih Chiu, Chun Yu Lin, Hua Lin Wu, Yuan Deng Su, Nan-Shan Chang, Ruei Yu He, and Shean-Jen Chen
- Subjects
Microscope ,Materials science ,Super-resolution microscopy ,business.industry ,Surface plasmon ,Cell Membrane ,Equipment Design ,Surface Plasmon Resonance ,Image Enhancement ,Atomic and Molecular Physics, and Optics ,law.invention ,Numerical aperture ,Equipment Failure Analysis ,Optics ,Microscopy, Fluorescence ,law ,Light sheet fluorescence microscopy ,Subtraction Technique ,Microscopy ,Fluorescence microscope ,Photoactivated localization microscopy ,Microscopy, Phase-Contrast ,business - Abstract
This paper demonstrates the first combination for wide-field surface plasmon (SP) phase microscopy and SP-enhanced fluorescence microscopy to image living cells' contacts on the surface of a bio-substrate simultaneously. The phase microscopy with a phase-shift interferometry and common-path optical setup can provide high-sensitivity phase information in long-term stability. Simultaneously, the fluorescence microscopy with the enhancement of a local electromagnetic field can supply bright fluorescent images. The combined microscope imposes a high numerical aperture objective upon the excitation of surface plasmon through a silver film with a thickness of 30 nm. The developed SP microscope is successfully applied to the real-time bright observation of the transfected fluorescence of living cells localized near the cell membrane on the bio-substrate and the high-sensitivity phase image of the cell-substrate contacts at the same time.
- Published
- 2010