1. Effect of LSD1 on osteogenic differentiation of human periodontal ligament stem cells in periodontitis.
- Author
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Shao, Qing, Liu, ShiWei, Zou, Chen, and Ai, Yilong
- Subjects
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CELL differentiation , *CELL culture , *BONE growth , *PERIODONTITIS , *MICRORNA , *GENE expression , *STEM cells , *HISTONES , *RESEARCH funding , *TRANSCRIPTION factors , *PERIODONTAL ligament - Abstract
Objective: Periodontitis is a chronic inflammation of periodontal tissues. This study is expected to assess the effect of LSD1 on the osteogenic differentiation of hPDLSCs in periodontitis. Methods: hPDLSCs were separated, cultivated, and identified, and then treated by LPS to induce inflammatory microenvironment and subjected to osteogenic differentiation. Subsequently, LSD1 expression was determined, and then silenced to assess its effect on hPDLSCs. Next, the binding relation between LSD1 and miR‐590‐3p was analyzed. miR‐590‐3p expression was detected and then overexpressed to evaluate its role in hPDLSCs in periodontitis. Afterward, the relation between LSD1 and OSX was analyzed. H3K4me2 level and OSX transcription were measured, and the role of H3K4me2 was determined. Additionally, the role of OSX in hPDLSCs was verified. Results: LSD1 was poorly expressed after osteogenic differentiation of hPDLSCs while it was rescued upon LPS induction. The osteogenic differentiation of hPDLSC in periodontitis was strengthened upon LSD1 downregulation. Besides, miR‐590‐3p targeted LSD1 transcription, and LSD1 inhibited OSX transcription via H3K4me2 demethylation. miR‐590‐3p overexpression improved osteogenic differentiation of hPDLSCs in periodontitis. But this improvement was annulled by OSX inhibition. Conclusion: miR‐590‐3p targeted LSD1 transcription and upregulated H3K4me2 methylation to promote OSX transcription, thereby encouraging osteogenic differentiation of hPDLSCs in periodontitis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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