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Abstract: Three Paleogene syn-rift intervals from the Bohai Bay Basin, the most petroliferous basin in China, were analyzed with sedimentological and geochemical techniques to characterize the lateral source rock heterogeneities, to reveal the environmental and ecological changes through geologic time and to construct depositional models for lacustrine source rocks under different tectonic and climatic conditions. The third (Es3) and first (Es1) members of the Eocene Shahejie Formation and the Oligocene Dongying Formation (Ed) display widely variable total organic carbon contents, hydrogen indices and visual kerogen compositions, suggesting changes in organic facies from deep to marginal sediments. Carefully selected deep-lake facies samples from any interval, however, display fairly uniform biomarker composition. These three intervals have distinctly different biomarker assemblages, which indicate weakly alkaline, freshwater lakes with a moderately deep thermocline during Es3 deposition, alkaline-saline lakes with shallow chemocline during Es1 deposition and acidic, freshwater lakes with deep, unstable thermocline during the deposition of the Dongying Formation. Such environmental changes corresponded to changes in subsidence rate and paleoclimate, from rapid subsidence and wet climate during Es3 deposition, through slow subsidence and arid climate during Es1 deposition to rapid subsidence and wet climate during Ed deposition and resulted in synchronous changes in terrigenous organic matter input, phytoplankton community and primary productivity. The co-evolution of environments and organisms controlled by tectonic subsidence and climate accounted for the deposition and distribution of high quality lacustrine source rocks with distinctly different geochemical characteristics. Most rift basins experienced changes in subsidence rates and possibly changes in climates during their syn-rift evolutions. The models constructed in this paper may have important implications for source rock prediction in other lacustrine rift basins. [Copyright &y& Elsevier]