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Your search keyword '"Kinya Hotta"' showing total 4 results
Start Over Author "Kinya Hotta" Journal organic letters
4 results on '"Kinya Hotta"'

1. Enzymatic Epoxide-Opening Cascades Catalyzed by a Pair of Epoxide Hydrolases in the Ionophore Polyether Biosynthesis

Author

Akira Migita, Kenji Watanabe, Hideaki Oikawa, Daiki Inada, Kinya Hotta, Atsushi Minami, and Hiroki Oguri

Subjects
Epoxide Hydrolases, chemistry.chemical_classification, Ionophores, Molecular Structure, Stereochemistry, Organic Chemistry, Mutagenesis, Ionophore, Epoxide, Biochemistry, Amino acid, chemistry.chemical_compound, Enzyme, Biosynthesis, chemistry, Biocatalysis, Epoxy Compounds, Physical and Theoretical Chemistry, Epoxide hydrolase, Ethers
Abstract

Our recent findings of the first epoxide hydrolase Lsd19, involved in lasalocid A biosynthesis, led us to investigate a long-standing controversial issue on the mechanism of enzymatic epoxide-opening cascades. The site-directed mutagenesis and domain dissection analysis to reveal the mechanism of the reaction catalyzed by Lsd19 is examined, especially in the role of acidic amino acid pair and catalytic domains.

Published
2011
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2. Elucidation of Pyranonigrin Biosynthetic Pathway Reveals a Mode of Tetramic Acid, Fused γ-Pyrone, and exo-Methylene Formation

Author

Tsuyoshi Yamamoto, Yuta Tsunematsu, Kinya Hotta, Kenji Watanabe, and Hiroshi Noguchi

Subjects
Stereochemistry, medicine.medical_treatment, Alkenes, Biochemistry, chemistry.chemical_compound, In vivo, Gene cluster, medicine, Molecule, Pyrroles, Physical and Theoretical Chemistry, Methylene, Gene knockout, Protease, biology, Molecular Structure, Organic Chemistry, Aspergillus niger, biology.organism_classification, Pyrone, Pyrrolidinones, Biosynthetic Pathways, chemistry, Pyrones, Multigene Family, Methane
Abstract

Successful activation of the pyranonigrin biosynthetic gene cluster and gene knockout in Aspergillus niger plus in vivo and in vitro assays led to isolation of six new products, including a spiro cyclobutane-containing dimeric compound, which served as the basis for the proposed comprehensive pyranonigrin biosynthetic pathway. Two redox enzymes are key to forming the characteristic fused γ-pyrone core, and a protease homologue performs the exo-methylene formation.

Published
2015

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