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In present research assessment of disease incidence was made between Bt and Non-Bt cotton against bacterial blight disease. Experiment was carried out in natural environmental conditions on twenty eight varieties/advance lines. Findings of the experiments depicted more disease incidence on Non-Bt varieties viz., Non Bt-FH 901 (41.7-43.4%), Non Bt-MNH 496 (47.6-49.5%), Non Bt-FH 1000 (48.6-51.2%), Non Bt-FH 207 (54.5-57.5%), Non BT-N 112 (54.7- 56.3%), Non BT-FH 942 (55.7-59.5%), Non BT-MNH 6070 (61.5-64.7%), Non Bt-FH 941 (63.4-61.6%), Non BT-N 814 (70.4-71.3%), Non Bt-FH 900 (74.7-77.4%), Non Bt-ANMOL (74.8-76.8%), Non Bt-FH 2015 (78.5-80.6%), Non BT REDACOLA (81.5-79.6%) and Non BT-C 26 (83.3-85.6%) while BT varieties BT-S-78 (25.8-26.7%), BT-Z-33 (26.7- 22.2%), BT-786 (28.6-31.5%), BT-A-ONE (28.8-26.5%), BT-282 (30.5-27.6%), BT-886 (33.5-35.3%), BT-3701 (38.3- 36. %), BT-SPECIAL (38.4-34.6%), BT-802 (41.3-43.4%), BT-92 (41.4-44.4%), BT-131 (43.8-41.7%), BT-905 (44.6- 42.3%), BT-SUPPER (45.6-46.8%) and BT-121 (48.6-52.4%) showed decreased response respectively. Hence, concluded that difference/variability in the resistant sources is present in Pakistan against the disease among BT varieties as compared to Non-Bt. [ABSTRACT FROM AUTHOR]