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Despite the importance of pastoralism, as an alternative or adjunct to farming in China's long history, a clear understanding of the spreading routes and mechanisms of pastoralism in China is lacking. Fungal spores can be a useful addition to zooarchaeological evidence and have been widely used to explore the origins and development of pastoralism in different regions of the globe. The northern Loess Plateau is one of the most important areas for the emergence and development of pastoralism in early China. The aim of this paper was to take the fungal spores in the Baihemiao (BHM) core that we extracted from a site in the Yulin region, combine them with the zooarchaeological records to explore their relationship with climate and environmental changes, and reconstruct the evolution of animal husbandry in the northern Loess Plateau since the mid-Holocene. The results indicate that the climatic conditions in the northern Loess Plateau during the first period of 6–4.2 ka were relatively humid, the number of domesticated herbivores was small, animal husbandry was not developed, and the spores of coprophilous fungi in the sediments mainly originated from wild herbivores. Fungal spores as well as zooarchaeological evidence reveal that cattle and sheep grazing developed rapidly in the region during the second period, c. 4.2–3 ka. We suggest that the arid climatic conditions during this period facilitated the development of pastoral activities in the northern Loess Plateau. However, this pastoral economy gradually weakened after 1.2 ka. • Sporormiella suggests a high volume of herbivore activities in the Baihemiao area in 6–4.2 ka. • Fungal spores and zooarchaeological record from the archaeological sites indicate that grazing activity was strong in 4.2-3 ka. • Arid climatic conditions facilitated the development of pastoral activities in the northern Loess Plateau in 4.2–3 ka. • Pastoralism weakened and declined during the period from 3 ka to 1.2 ka. [ABSTRACT FROM AUTHOR]