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Abstract The Eocene was an interval of climate evolution, when the extreme high temperatures of the earliest Cenozoic gave way to a global cooling trend. Fine-grained lacustrine sediments of Eocene age from northeastern China represent an excellent archive to study long-term environmental and climatic changes at high-resolution. The Huadian Basin is a Cenozoic, fault-bounded basin in Jilin Province, northeastern China. Though small in area, it contains numerous oil shale- and coal-bearing layers, which provide an abundant Middle to Late Eocene palynoflora. Eighty-five palynomorph genera and one hundred palynomorph species were identified in the Huadian Formation, allowing to distinguish 4 palynozones. The nearest living relatives of the recovered palynomorph taxa suggest the presence of subtropical evergreen and deciduous broad-leaved forests during deposition of the Huadian Formation. Using the coexistence approach (CA), we calculated seven paleoclimatic parameters, which indicate a subtropical warm and humid climate with a mean annual temperature above 13.6 °C and a mean annual precipitation of at least 887 mm. However, the climate became cooler and drier during the transition from the lower Oil Shale Member to the upper Carbonaceous Shale Member. Palaeoclimatic data from the various Middle and Late Eocene basins of northeastern China (i.e., the Huadian, Fushun, Yilan, Hunchun and Jishu basins) were compared with data from North American and European basins. Middle Eocene climate in the Northern Hemisphere was generally warm and humid, and the mean annual temperature and precipitation in northeastern China were similar to those in North America, though slightly lower than those of Europe. Similar trends in temperature and precipitation on different continents support the existence of a global Mid-Eocene cooling event. This paper is the first systematic study of Middle to Late Eocene seasonality in the Huadian Basin. Our results show an apparent differentiation in both temperature and precipitation between summer and winter, supporting the view that the East Asian Monsoon was established in northeastern China already during the Middle to Late Eocene. This Middle and Late Eocene monsoon was likely driven by the development of Pacific trade winds and an alternating, seasonal high- and low-pressure system over Siberia. The climatic changes observed in our dataset, especially the precipitation changes, appear to have played an important role in controlling oil shale formation in the Huadian Basin. A warmer and more humid climate, with abundant precipitation and high subsidence rates, formed a deeper Huadian depositional lake. High productivity and stable stratification provided the abundant organic matter and favorable preservation conditions necessary for oil shale accumulation. Highlights • The Middle-Late Eocene palynoflora were firstly systematically studied in Huadian Basin. • We quantitatively estimated the Middle-Late Eocene climate based on CA method. • A globally cooler phase was recognized in the Middle Eocene. • Climate seasonality in the Middle-Late Eocene supports the EAM was already prevalent in NE China. • Changes in climate, especially precipitation, play an important role in oil shale formation. [ABSTRACT FROM AUTHOR]