1. NYD-OP7/PLC regulatory signaling pathway regulates deltamethrin resistance in Culex pipiens pallens (Diptera: Culicidae).
- Author
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Zhou D, Duan B, Xu Y, Ma L, Shen B, Sun Y, and Zhu C
- Subjects
- Animals, Culex genetics, Culex metabolism, Cytochrome P-450 Enzyme System metabolism, Gene Expression Regulation, Gene Knockdown Techniques, Insecticides pharmacology, Signal Transduction physiology, Culex drug effects, Insect Proteins metabolism, Insecticide Resistance genetics, Nitriles pharmacology, Pyrethrins pharmacology, Signal Transduction drug effects
- Abstract
Background: Investigation of insecticide resistance mechanisms is considered a vital first step towards the creation of effective strategies to control resistant mosquitoes and manage mosquito-borne diseases. Our previous study revealed that NYD-OP7 may be associated with deltamethrin resistance in Culex pipiens pallen. However, the precise function of NYD-OP7 in deltamethrin resistance is still unclear. In this study, we investigated the role of NYD-OP7 in the molecular mechanisms underlying pyrethroid resistance., Results: Knockdown of NYD-OP7 not only increased the susceptibility of the mosquitoes to deltamethrin in vivo but also simultaneously repressed both expression and enzyme activity of its downstream effector molecule, phospholipase C (PLC) and expression of several insecticide resistance-related P450 genes. Knockdown of PLC also sensitized the mosquitoes to deltamethrin and reduced the expression of the P450 genes., Conclusions: Our results revealed that NYD-OP7 and its downstream effector PLC contribute to deltamethrin resistance by regulating the expression of P450s in Cx. pipiens pallens.
- Published
- 2018
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