6 results on '"Pinlaor S"'
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2. α-Tocopherol and lipid profiles in plasma and the expression of α-tocopherol-related molecules in the liver of Opisthorchis viverrini-infected hamsters.
- Author
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Laothong U, Pinlaor P, Boonsiri P, Hiraku Y, Khoontawad J, Hongsrichan N, Charoensuk L, and Pinlaor S
- Subjects
- Animals, Chromatography, Thin Layer, Cricetinae, Disease Models, Animal, Gene Expression Regulation, Lipid Metabolism, Lipoproteins blood, Liver chemistry, Liver metabolism, Male, Mesocricetus, Oxidative Stress, ATP-Binding Cassette Transporters metabolism, Carrier Proteins metabolism, Lipids blood, Opisthorchiasis parasitology, Opisthorchis physiology, alpha-Tocopherol blood
- Abstract
Opisthorchis viverrini infection induces inflammation-mediated oxidative stress and liver injury, which may alter α-tocopherol and lipid metabolism. We investigated plasma α-tocopherol and lipid profiles in hamsters infected with O. viverrini. Levels of α-tocopherol, cholesterol, and low-density lipoprotein increased in the acute phase of infection. In the chronic phase, α-tocopherol decreased, while triglyceride and very low-density lipoprotein increased. Notably, high-density lipoprotein decreased both in the acute and chronic phases. In the liver, cholesteryl oleate, triolein, and oleic acid decreased in the acute phase, and increased in the chronic phase. Such chronological changes were negatively correlated with the plasma α-tocopherol level. The expression of α-tocopherol-related molecules, ATP-binding cassette transporter A1 (ABCA1) and α-tocopherol transfer protein, increased throughout the experiment. These results suggest that O. viverrini infection profoundly affects on lipid and α-tocopherol metabolism in due course of infection., (Crown Copyright © 2012. Published by Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
- Full Text
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3. The liver fluke Opisthorchis viverrini expresses nitric oxide synthase but not gelatinases.
- Author
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Prakobwong S, Pinlaor P, Charoensuk L, Khoontawad J, Yongvanit P, Hiraku Y, and Pinlaor S
- Subjects
- Animals, Cricetinae, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Indirect veterinary, Gelatinases metabolism, Heme Oxygenase-1 metabolism, Liver parasitology, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism, Mesocricetus, Nitric Oxide metabolism, Opisthorchiasis enzymology, Organ Specificity, rac1 GTP-Binding Protein metabolism, Helminth Proteins metabolism, Nitric Oxide Synthase metabolism, Opisthorchiasis parasitology, Opisthorchis enzymology
- Abstract
Host-parasite interaction during infection with the liver fluke Opisthorchis viverrini plays an important role in opisthorchiasis-associated cholangiocarcinoma via nitric oxide (NO) production. Host cells induce nitric oxide synthase (NOS)-dependent DNA damage and secrete Ras-related C3 botulinum toxin substrate (Rac)1, heme oxygenase (HO)-1, and gelatinases (matrix metalloproteinase (MMP)2 and MMP9). We evaluated whether these enzymes are expressed in O. viverrini. Colocalization of NOS and Rac1 was most prominently detected on day 30 post-infection (p.i.) in the gut, reproductive organ, eggs, acetabular and tegument. Expression of HO-1, an antioxidative enzyme, increased in a similar pattern to NOS, but was not present in the tegument. The levels of nitrate/nitrite, end products of NO, and ferric reducing antioxidant capacity, an indicator of antioxidant enzyme capacity, in parasite homogenates were highest on day 30 p.i. and then decreased on day 90 p.i. In contrast, zymography revealed that MMP2 and MMP9 were not present in parasite homogenates at all time points. In conclusion, O. viverrini induces NOS expression and NO production, but does not express gelatinases. The study may provide basic information and an insight into drug design for prevention and/or intervention approaches against O. viverrini infection., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
4. Oxidative and nitrative DNA damage: key events in opisthorchiasis-induced carcinogenesis.
- Author
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Yongvanit P, Pinlaor S, and Bartsch H
- Subjects
- Animals, Anthelmintics therapeutic use, Bile Duct Neoplasms immunology, Bile Duct Neoplasms parasitology, Bile Duct Neoplasms pathology, Bile Ducts, Intrahepatic immunology, Bile Ducts, Intrahepatic parasitology, Bile Ducts, Intrahepatic pathology, Cholangiocarcinoma immunology, Cholangiocarcinoma parasitology, Cholangiocarcinoma pathology, Cyclooxygenase 2 metabolism, Cytokines metabolism, DNA Damage, Disease Models, Animal, Humans, Lipid Peroxidation, NF-kappa B metabolism, Nitric Oxide Synthase Type II metabolism, Opisthorchiasis drug therapy, Opisthorchiasis pathology, Praziquantel therapeutic use, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism, Risk Factors, Rodentia, Thailand, Bile Duct Neoplasms physiopathology, Cholangiocarcinoma physiopathology, Opisthorchiasis complications, Opisthorchiasis parasitology, Opisthorchis physiology, Oxidative Stress
- Abstract
Chronic inflammation induced by liver fluke (Opisthorchis viverrini) infection is the major risk factor for cholangiocarcinoma (CCA) in Northeastern Thailand. Increased levels of proinflammatory cytokines and nuclear factor kappa B that control cyclooxygenase-2 and inducible nitric oxide activities, disturb the homeostasis of oxidants/anti-oxidants and DNA repair enzymes, all of which appear to be involved in O. viverrini-associated inflammatory processes and CCA. Consequently oxidative and nitrative stress-related cellular damage occurs due to the over production of reactive oxygen and nitrogen species in inflamed target cells. This is supported by the detection of high levels of oxidized DNA and DNA bases modified by lipid peroxidation products in both animal and human tissues affected by O. viverrini-infection. Treatment of opisthorchiasis patients with praziquantel, an anti- trematode drug was shown to reduce inflammation-mediated tissue damage and carcinogenesis. The principal mechanisms that govern the effects of inflammation and immunity in liver fluke-associated cholangiocarcinogenesis are reviewed. The validity of inflammation-related biomolecules and DNA damage products to serve as predictive biomarkers for disease risk evaluation and intervention is discussed., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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5. Specific serum IgG, but not IgA, antibody against purified Opisthorchis viverrini antigen associated with hepatobiliary disease and cholangiocarcinoma.
- Author
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Pinlaor P, Pongsamart P, Hongsrichan N, Sangka A, Srilunchang T, Mairiang E, Sithithaworn P, and Pinlaor S
- Subjects
- Adult, Animals, Bile Duct Neoplasms blood, Bile Duct Neoplasms complications, Bile Duct Neoplasms diagnostic imaging, Bile Duct Neoplasms immunology, Bile Ducts, Intrahepatic diagnostic imaging, Bile Ducts, Intrahepatic immunology, Bile Ducts, Intrahepatic pathology, Biliary Tract diagnostic imaging, Biliary Tract immunology, Biliary Tract pathology, Biliary Tract Diseases blood, Biliary Tract Diseases complications, Biliary Tract Diseases diagnostic imaging, Biliary Tract Diseases immunology, Cholangiocarcinoma blood, Cholangiocarcinoma complications, Cholangiocarcinoma diagnostic imaging, Cholangiocarcinoma immunology, Chromatography, Gel, Cricetinae, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Female, Fishes parasitology, Humans, Immunoglobulin A blood, Immunoglobulin G blood, Linear Models, Liver diagnostic imaging, Liver immunology, Liver pathology, Liver Diseases blood, Liver Diseases complications, Liver Diseases diagnostic imaging, Liver Diseases immunology, Male, Middle Aged, Opisthorchiasis diagnosis, Species Specificity, Thailand, Ultrasonography, Antibodies, Helminth blood, Antigens, Helminth immunology, Opisthorchiasis immunology, Opisthorchis immunology
- Abstract
Opisthorchiasis caused by Opisthorchis viverrini infection induces hepatobiliary disease (HBD)-associated cholangiocarcinoma (CCA) via a chronic inflammatory immune response. Here, we evaluated specific IgG and IgA antibodies against different fractions of O. viverrini antigen in residents from an endemic community in Northeast Thailand with varying hepatobiliary abnormalities. Crude somatic O. viverrini antigen was purified into three fractions (viz., P1, P2 and P3) by gel infiltration chromatography and these served as antigens for detection of fluke-specific IgG and IgA antibodies by enzyme-linked immunosorbent assay (ELISA). The results revealed fluke-specific IgG and IgA antibody levels-against these antigens from subjects with O. viverrini-positive HBD-higher than in subjects with O. viverrini-negative HBD. Interestingly, the rank of fluke-specific IgG (and not IgA) antibody levels against crude extract and P1 antigens was CCA>severe HBD>mild HBD>healthy individuals. Purified antigens reduced cross-reactivity with other parasites compared to the crude antigen. Multiple linear regression analysis showed that HBD status was significantly associated with the liver fluke-specific IgG antibody against purified antigens. These results suggest that purified O. viverrini-antigen improves serodiagnosis for the evaluation of opisthorchiasis-associated HBD, and may be useful in the screening of opisthorchiasis in subjects at risk of developing CCA., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
6. Diagnostic values of parasite-specific antibody detections in saliva and urine in comparison with serum in opisthorchiasis.
- Author
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Sawangsoda P, Sithithaworn J, Tesana S, Pinlaor S, Boonmars T, Mairiang E, Yongvanit P, Duenngai K, and Sithithaworn P
- Subjects
- Acetates chemistry, Adult, Aged, Aged, 80 and over, Animals, Antibodies, Helminth blood, Antibodies, Helminth urine, Antigens, Helminth blood, Antigens, Helminth urine, Area Under Curve, Cholangiocarcinoma immunology, Feces parasitology, Female, Formaldehyde chemistry, Humans, Immunoglobulin A analysis, Immunoglobulin A blood, Immunoglobulin A urine, Immunoglobulin G analysis, Immunoglobulin G blood, Immunoglobulin G urine, Immunoglobulins blood, Immunoglobulins urine, Male, Middle Aged, Opisthorchiasis immunology, ROC Curve, Saliva immunology, Sensitivity and Specificity, Thailand, Antibodies, Helminth analysis, Antigens, Helminth analysis, Cholangiocarcinoma diagnosis, Enzyme-Linked Immunosorbent Assay methods, Immunoglobulins analysis, Opisthorchiasis diagnosis, Opisthorchis immunology
- Abstract
Infection by the liver fluke (Opisthorchis viverrini) causes hepatobiliary disease and bile duct cancer (cholangiocarcinoma, CCA) in endemic areas in Southeast Asia. Measurements of humoral immune response particularly parasite-specific antibodies are useful not only for serodiagnosis but they have been implicated as risk factors of CCA. In this study, we used indirect Enzyme Immunosorbent Assay (ELISA) to measure O. viverrini-specific immunoglobulins in serum, urine and saliva and assessed efficacies in diagnosis of opisthorchiasis and evaluated the relationship of antibodies among clinical specimens in a sample population in endemic areas in Khon Kaen, Thailand. By employing the Receiver Operation Characteristics (ROC) analysis, diagnostic efficacy based upon the area under the curve (AUC) revealed that serum, salivary IgG and IgA performed better than urine for diagnosis of opisthorchiasis. Seropositive cases were found in both parasite egg-negative as well as O. viverrini egg-positive groups. The levels of serum IgG correlated with intensity of O. viverrini infection (P<0.05). Diagnostic sensitivities based on serum and salivary IgG, IgA also positively associated with the intensity of infection. Correlations between serum antibodies and those in saliva were found to be greater in egg-negative than egg-positive individuals for O. viverrini. Our findings indicated a complex interrelation between antibody responses in different clinical specimens triggered by liver fluke infection. More comprehensive examinations are needed to determine the potential utility of salivary antibody detection which, in combination with the conventional fecal examination method, may better assist in the identification of individuals with opisthorchiasis. Furthermore, it may provide a better indicator of the risk of disease, particularly CCA., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
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