1. In vitro antiplasmodial and phytochemical study of five Artemisia species from Iran and in vivo activity of two species
- Author
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Ali Ramazani, Soroush Sardari, Sedigheh Zakeri, Behrouz Vaziri, Biotechnology Research Center, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Drug Design and Bioinformatics Unit, Authors wish to thank Pasteur Institute of Iran for partial financial funding and support of the project (Grant no. 289), Ali Ramazani, Soroush Sardari, Sedigheh Zakeri, and Behrouz Vaziri
- Subjects
Magnetic Resonance Spectroscopy ,Plasmodium berghei ,phytochemical ,Pharmacology ,Artemisia annua ,Iran ,MESH: Parasitic Sensitivity Tests ,Absinthium ,Mice ,0302 clinical medicine ,Parasitic Sensitivity Tests ,MESH: Animals ,Artemisinin ,MESH: Plasmodium falciparum ,Chromatography, High Pressure Liquid ,0303 health sciences ,Mice, Inbred BALB C ,biology ,Traditional medicine ,food and beverages ,General Medicine ,Artemisinins ,Drug Resistance, Multiple ,3. Good health ,Artemisia species ,in vivo ,Infectious Diseases ,MESH: Chromatography, Thin Layer ,Phytochemical ,MESH: Artemisia annua ,Female ,medicine.drug ,030231 tropical medicine ,MESH: Malaria ,Plasmodium falciparum ,MESH: Mice, Inbred BALB C ,MESH: Plant Extracts ,Artemisia absinthium ,MESH: Drug Resistance, Multiple ,03 medical and health sciences ,Antimalarials ,In vitro ,Species Specificity ,antiplasmodial ,parasitic diseases ,MESH: Artemisinins ,medicine ,MESH: Plasmodium berghei ,MESH: Species Specificity ,Animals ,MESH: Artemisia absinthium ,MESH: Chromatography, High Pressure Liquid ,MESH: Mice ,030304 developmental biology ,General Veterinary ,MESH: Magnetic Resonance Spectroscopy ,Plant Extracts ,biology.organism_classification ,MESH: Antimalarials ,Malaria ,Multiple drug resistance ,Artemisia ,Insect Science ,MESH: Iran ,[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology ,Parasitology ,Chromatography, Thin Layer ,MESH: Artemisia ,MESH: Female - Abstract
International audience; The extract from Artemisia annua, containing artemisinin, has been proven active against multidrug resistant Plasmodium falciparum in previous studies. The purpose of this paper was to study five Artemisia species from Iran for their in vitro and in vivo antimalarial property and detection of artemisinin in the active species by chromatographic and spectroscopic methods including nuclear magnetic resonance (NMR) spectroscopy. Dried plants were extracted by 80% ethanol, and total extracts were investigated for antiplasmodial property and artemisinin content by TLC, HPLC, and (1)H-NMR techniques. Two plants (A. annua L. and Artemisia absinthium L.) showed good antiplasmodial activity against multidrug resistant and sensitive strain of P. falciparum. A. absinthium and A. annua at concentrations of 200 mg/kg for 4 days reduced parasitemia in BALB/C mice infected with Plasmodium bergei by 94.28% and 83.28%, respectively, but we could not detect artemisinin in all plants studied in this research. The antiplasmodial property of these two herbs is possibly related to essential oils that present in high amounts in their extracts.
- Published
- 2009
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