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When we were in school, we were taught how to use a note book and a pen to determine central tendency and dispersion. The entire process took too long and required too much time. These days, we can accomplish this using a programme called biostatistical software. These programmes reduce labour costs as well as time. India consistently ranks as one of the top five pharmaceutical producing nations in the world. The use of software, which allows a work to be completed with less effort and more accuracy, is growing steadily. In research, the use of computers and innovative software is becoming more and more frequent, particularly in the field of biostatistics, which deals with the application of mathematics to biological systems (living things). Due to its usage in both experimental and observational research, biostatistics is advantageous not only to the pharmaceutical business but also to medical and paramedical schools (such as nursing and pharmacy schools). Pen and paper calculations and measurements take an enormous amount of time and are inefficient. Currently, a variety of statistical software is available for the fulfilment of tasks that can quickly produce results. Software programmes used for statistical analysis include Minitab, SPSS, R studio and Microsoft Excel. This article will go over various biostatistical software programmes and how to utilise them to calculate central tendency and dispersion. Because it is free, versatile and simple to use MS Excel is the biostatistical programme that is utilised the most. Cons and benefits can be found in every piece of software. Microsoft Excel is free, whereas other programmes require a monthly or annual fee. There are available demo versions of these that can be used for educational purposes