1. La voie IL-2/IL-2R chez la cellule dendritique module à la fois leur synthèse de cytokines et leur capacité à activer des lymphocytes T CD4+ allogéniques
- Author
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Mnasria, K., Lagaraine, C., Manaa, J., Lebranchu, Y., and Oueslati, R.
- Subjects
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INTERLEUKIN-2 , *BIOSYNTHESIS , *CYTOKINES , *DENDRITIC cells , *GENE expression , *MONOCLONAL antibodies , *CELLULAR signal transduction , *HOMOGRAFTS , *T cells , *CELL proliferation , *GRAFT rejection - Abstract
Abstract: The results provide new insights into the role of IL-2/IL-2R pathway in DC. We report that stimulation of human monocyte-derived DC with LPS strongly upregulated CD25 (α chain of the IL-2R) expression. In addition, by using a humanized monoclonal antibody against CD25, we demonstrated that the IL-2 signalling in DC upregulated both IL-12 and γIFN production but decreased IL-10 synthesis. Anti-CD25 treatment reduced the ability of LPS-DC to induce allogeneic CD4+ T cell proliferation as compared to LPS-matured DC. In addition, LPS-matured DC treated with IL-2 had a higher allostimulatory capacity compared to LPS-DC. We also found that LPS-matured DC produced IL-2. Thus, IL-2 seems to contribute actively to DC activation through an autocrine pathway. Moreover, IL-2 pathway in DC is involved in T helper priming. These findings might be useful for protocols in cellular therapy and a valuable tool to understand graft rejection versus the acquisition of peripheral tolerance. [Copyright &y& Elsevier]
- Published
- 2011
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