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Abstract: We studied the sensitivity of the hospital environmental bacterial strains to Surfanios®, wich is a detergent and disinfectant product for surfaces. This product is used in our establishment since nearly 10 years. This work which relates to 425 bacterial strains proposes to study the possible evolution of the resistance of the bacteria under the pressure of a product biocide used since many years without the change of active ingredient as recommended in pharmaceutical and agroalimentary industry. We developed a micromethod to study the sensitivity of many bacterial strains simultaneously into a 96 wells microplaque. All the Staphylococcus aureus strains (N =20) and Staphylococcus with coagulase negative (N =308) as 78 Acinetobacter sp. strains remain very sensitive to Surfanios® according to our study. Target dilution, which is the last dilution not allowing the bacterial growth, is much lower than the manufacturer recommended use dilution. For the Pseudomonas aeruginosa strains tested, target dilution is equal or higher than the dilution of use. Rather than to propose the rotation or the alternation of the products, we recommend the use of Surfanios® for the bionettoyage of dry surfaces which might be contaminated by Staphylococcus sp. or Acinetobacter sp. and the use of another product or Surfanios® at a higher concentration, active on Pseudomonas sp. to disinfect wet surfaces which are possible reservoirs for the opportunist bacteria of the hydrous flora. [Copyright &y& Elsevier]

Abstract: Objective: Study of the clonality of methicillin-resistant Staphylococcus haemolyticus responsible of epidemic infections in a neonatal intensive care unit. Patients and methods: Methicillin-resistant Staphylococcus haemolyticus isolates were collected during the period from March 2004 to November 2006, from newborns, the clean hands of nurses and from disinfectant bottles used in the unit. Molecular typing by pulsed-field gel electrophoresis (PFGE) was achieved for all isolates. Results: Forty-six isolates of S. haemolyticus resistant to methicillin were collected from 42 newborns, the hand of two nurses and from two disinfectant bottles used in the unit. PFGE analysis revealed five types (A, B, C, D and E) among newborns isolates. Types A and B were predominant. Nurses’ isolates revealed PFGE types similar to types A and B. Disinfectant isolates were of type B. qacA/B PCR analysis revealed that the majority of type B isolates contain the disinfectant resistance gene qacA/B. No isolate of type A possessed this gene. Conclusion: These results suggest that MRSH neonatal infections are caused by a limited number of clones. Clone B was able to survive in disinfectant bottles and to conserve its ability to infect newborns. We therefore conclude that the disinfectant can serve as a reservoir for MRSH and point out the need to control all disinfectants used in a neonatal intensive care unit. [Copyright &y& Elsevier]