1. Expression of SOX11, PAX5, TTF-1 and ISL-1 in medulloblastoma.
- Author
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Czapiewski P, Gorczynski A, Radecka K, Wiewiora C, Haybaeck J, Adam P, Fend F, Zakrzewska M, Zakrzewski K, Liberski PP, and Biernat W
- Subjects
- Adolescent, Adult, Biomarkers, Tumor analysis, Cerebellar Neoplasms metabolism, Child, Child, Preschool, DNA-Binding Proteins analysis, Female, Humans, Immunohistochemistry, Infant, LIM-Homeodomain Proteins analysis, Male, Medulloblastoma metabolism, PAX5 Transcription Factor analysis, SOXC Transcription Factors analysis, Transcription Factors analysis, Young Adult, Cerebellar Neoplasms diagnosis, DNA-Binding Proteins biosynthesis, LIM-Homeodomain Proteins biosynthesis, Medulloblastoma diagnosis, PAX5 Transcription Factor biosynthesis, SOXC Transcription Factors biosynthesis, Transcription Factors biosynthesis
- Abstract
The aim of our study was to evaluate the immunohistochemical expression of SOX11, PAX5, TTF-1 and ISL-1 in medulloblastoma (MB) to investigate their diagnostic usefulness., Methods: Immunohistochemical expression of PAX5 (two antibodies: Dako, DAK-Pax5; and BD, clone 24), TTF-1 (Dako, 8G7G3/1), SOX11 (CL0142; Abcam) and ISL-1 (1 H9, Abcam) was analyzed using the h-score and Remmele score in 25 cases of MB., Results: There were 18 MBs of classic and 7 of desmoplastic type. SOX11 was strongly expressed in all tumors. The expression of PAX5 was higher and more frequent in a case of DAK-Pax5 clone (25/25) than clone 24 (6/25). ISL-1 was positive in 11 (44%) and TTF-1 in 3 (12%) cases. ISL-1 expression correlated positively (p<0.001), while TTF-1 correlated negatively with the age of patients (p=0.039). PAX5 expression correlated with ISL-1 (p=0.039) and showed a trend toward higher expression in the desmoplastic subtype (p=0.069)., Conclusions: SOX11 is strongly and robustly expressed in MBs. PAX5 expression pattern differs substantially among two antibody clones. TTF-1 and ISL-1 is associated with the age of patients., (Copyright © 2016 Elsevier GmbH. All rights reserved.)
- Published
- 2016
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