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As molecular chaperones, DnaJs play critical roles in maintaining cytoplasmic structure and resisting various stresses. However, the functions of DnaJs in insects are poorly understood. In this study, we identified a DnaJC3 from Apis cerana cerana (AccDnaJC3) and investigated its roles in adverse conditions. Real-time quantitative PCR analysis showed that AccDnaJC3 was highly expressed in muscle and epidermis. In addition, AccDnaJC3 was induced by a variety of stresses, such as 4 °C, 24 °C, 44 °C, H 2 O 2 , HgCl 2 , VC, UV, cyhalothrin, abamectin and emamectin benzoate treatments, whereas it was inhibited by CdCl 2 and paraquat treatments. Disc diffusion experiments indicated that overexpression of recombinant AccDnaJC3 enhanced Escherichia coli tolerance to some stress conditions. In contrast to the control group, when AccDnaJC3 was knocked down with RNAi technology, several other antioxidant genes were downregulated, suggesting that AccDnaJC3 may play important roles in stress response. Furthermore, we found that the enzyme activities of superoxide dismutase, peroxidase and catalase were lower in AccDnaJC3 -knockdown bees than in control bees. Taken together, these results suggest that AccDnaJC3 may be involved in various stress responses in Apis cerana cerana. Unlabelled Image • A DnaJC3 gene from Apis cerana cerana was isolated and characterized. • The expression of AccDnaJC3 was induced by various environmental stresses. • AccDnaJC3 enhances the tolerance of Escherichia coli to stress conditions. • RNA interference experiments suggested that AccDnaJC3 is important for resistance against stresses. [ABSTRACT FROM AUTHOR]

Insect cytochrome P450 monooxygenases (P450s or CYPs) perform important functions in the metabolic detoxification of both endogenous and exogenous substrates. However, the mechanism of action of the P450 genes in bees is unclear. In this study, we investigated the effects of AccCYP6k1 on the metabolism and detoxification of Apis cerana cerana. Spatiotemporal expression profiling revealed that the expression of AccCYP6k1 was the highest in foragers (A15) and was mainly expressed in the leg, midgut and head. RT–qPCR results showed that AccCYP6k1 exhibited different expression patterns following exposure to xenobiotics. In addition, silencing AccCYP6k1 increased the pesticides sensitivity and affected the detoxification system and antioxidant process of A. cerana cerana. In brief, the induced expression of AccCYP6k1 is related to the resistance of A. cerana cerana , while knockdown AccCYP6k1 affect the pesticides resistance and metabolic detoxification system of A. cerana cerana. These findings not only support the theoretical basis of metabolic detoxification in bees but also provide a better understanding of P450-mediated resistance to pesticides in insects. [Display omitted] • The level of inducible expression of AccCYP6k1 is related to pesticide resistance. • Silencing AccCYP6k1 increased the sensitivity of Apis cerana cerana to abamectin and imidacloprid. • Silencing AccCYP6k1 had adverse effects on the detoxification system of Apis cerana cerana. [ABSTRACT FROM AUTHOR]

Tyrosine aminotransferase (TATN) is the first enzyme involved in the metabolic degradation of tyrosine, and it plays an important role in tyrosine detoxification and helps the body resist oxidative damage. However, the function of TATN in Apis cerana cerana (A. c. cerana) remains unclear. To explore the role of TATN in the response to pesticide and heavy metal stress in A. c. cerana , AccTATN was isolated and identified. AccTATN was highly expressed in the integument and the adult stage. Exposure to multiple pesticides and heavy metal stress upregulated AccTATN expression. RNA interference experiments showed that silencing AccTATN reduced the resistance of A. c. cerana to glyphosate and avermectins stress. The expression of antioxidant-related genes and the activity of antioxidant enzymes were reduced after AccTATN was silenced, leading to the accumulation of oxidative damage. Overexpression of the recombinant AccTATN protein in a prokaryotic system also confirmed its role in heavy metal stress and improved antioxidant capacity. Our study showed that AccTATN may promote resistance to pesticide and heavy metal stress by regulating the antioxidant capacity of A. c. cerana. This study provides a valuable theoretical basis for A. c. cerana conservation. [Display omitted] • AccTATN gene was isolated and characterized from (A. c. cerana) • Pesticide and heavy metal stress can affect the expression of AccTATN. • AccTATN overexpression enhanced the tolerance of E. coli cells to oxidative stress. • AccTATN knockdown disrupted the original redox balance and caused oxidative stress. • AccTATN promotes A. c. cerana to resist pesticide and heavy metal stress. [ABSTRACT FROM AUTHOR]

Activating transcription factor 2 (ATF2), a basic leucine zipper (bZIP) transcription factor, plays a crucial role in immune and DNA damage response in mammals. However, the function of ATF2 in insects remains unknown. Here, we isolated the ATF2 gene from Apis cerana cerana (AccATF2) and found that AccATF2 was a main regulator of the honeybee response to oxidative stress. Our results showed that AccATF2 was highly expressed in the head, thorax and integument. AccATF2 was expressed throughout the development period of honeybees, and the highest AccATF2 transcript level was noted in brown-eyed pupae, indicating its indispensable roles in honeybee survival. Antioxidant function analysis showed that AccATF2 expression was markedly induced in response to oxidative stress caused by various environmental stresses. AccATF2 overexpression substantially enhanced the tolerance to oxidative stress of Escherichia coli cells compared with control cells. AccATF2 knockdown significantly increased the production of malondialdehyde (MDA), the transcription of antioxidant genes and the activity of antioxidant enzymes in honeybees, suggesting that AccATF2 knockdown resulted in oxidative damage to honeybees. Moreover, AccATF2 knockdown decreased honeybee resistance to oxidative stress caused by high temperature. Overall, AccATF2 plays an important role in maintaining redox homeostasis and protecting honeybees from oxidative stress caused by various environmental stimuli. Our discoveries add to a growing understanding of how honeybees cope with various adverse environmental conditions to ensure their survival. [Display omitted] • AccATF2 gene was isolated and characterized from Apis cerana cerana. • The expression of AccATF2 was induced by oxidative stress caused by adverse environment. • AccATF2 overexpression enhanced the tolerance of E. coli cells to oxidative stress. • AccATF2 knockdown disrupted the original redox balance and caused oxidative stress. [ABSTRACT FROM AUTHOR]

The cyclin-dependent kinase (CDK) protein family plays an important role in regulating life functions, such as the cell cycle and metabolism. This study reports the first cloning and functional analysis of A. cerana cerana CDK1 (AccCDK1). The distribution profile of AccCDK1 in different developmental periods and different tissues was determined. The experimental results showed that the distribution of AccCDK1 was tissue-specific. AccCDK1 distribution at the transcriptional and translational levels was affected by stress conditions induced by H 2 O 2 , UV, HgCl 2 , CdCl 2 , extreme temperatures (4 °C, 44 °C) and pesticides (avermectin, lambda-cyhalothrin, haloxyfop- R -methyl, and glyphosate), which resulted in changes in the expression levels. These results suggest that AccCDK1 may have an important part to play in honey bee resistance to stress. The expression of a recombinant AccCDK1 protein in vitro enhanced the antistress capacities of E. coli and yeast, which suggests that AccCDK1 is related to the stress response. When AccCDK1 was silenced, the expression of some antioxidant genes was downregulated, and the enzymatic potencies of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were reduced, which suggests that AccCDK1 takes part in the body's resistance to oxidative stress upon external stimulation by influencing relevant antioxidants. Notably, the survival rate of A. cerana cerana under high-temperature-induced stress decreased after AccCDK1 silencing, which verifies our results. In conclusion, we found that AccCDK1 played an indispensable function in resisting oxidative stress and maintaining normal cellular functions. [Display omitted] • Oxidative stress can affect the expression of cyclin-dependent kinase1. • AccCDK1 silencing affects cellular antioxidant genes and enzymes. • AccCDK1 promotes the survival of eukaryotic and prokaryotic cells under stress. • AccCDK1 promotes bees to resist high temperature stress. [ABSTRACT FROM AUTHOR]