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Your search keyword '"Tamara Regina Calvo"' showing total 4 results
Start Over Author "Tamara Regina Calvo" Journal pharmaceutical biology
4 results on '"Tamara Regina Calvo"'

1. Effects of indirubin and isatin on cell viability, mutagenicity, genotoxicity and BAX/ERCC1 gene expression

Author

Manoela Viar Fogaça, Priscila de Matos Cândido-Bacani, Lucas Milanez Benicio, Lara Martinelli Zapata, Priscilla de Freitas Cardoso, Marcelo Tempesta de Oliveira, Tamara Regina Calvo, Eliana Aparecida Varanda, Wagner Vilegas, and Ilce Mara de Syllos Cólus

Subjects
indigofera suffruticosa, indigofera truxillensis, hela cells, cho-k1 cells, cytokinesis-blocked micronucleus assay, comet assay, apoptosis, Therapeutics. Pharmacology, RM1-950
Abstract

Context: Indigofera suffruticosa Miller (Fabaceae) and I. truxillensis Kunth produce compounds, such as isatin (ISA) and indirubin (IRN), which possess antitumour properties. Their effects in mammalian cells are still not very well understood. Objective: We evaluated the activities of ISA and/or IRN on cell viability and apoptosis in vitro, their genotoxic potentials in vitro and in vivo, and the IRN- and ISA-induced expression of ERCC1 or BAX genes. Materials and methods: HeLa and/or CHO-K1 cell lines were tested (3 or 24 h) in the MTT, Trypan blue exclusion, acridine orange/ethidium bromide, cytokinesis-blocked micronucleus (CBMN) and comet (36, 24 and 72 h) tests after treatment with IRN (0.1 to 200 μM) or ISA (0.5 to 50 μM). Gene expression was measured by RT-qPCR in HeLa cells. Swiss albino mice received IRN (3, 4 or 24 h) by gavage (50, 100 and 150 mg/kg determined from the LD50 – 1 g/kg b.w.) and submitted to comet assay in vivo. Results: IRN reduced the viability of CHO-K1 (24 h; 5 to 200 μM) and HeLa cells (10 to 200 μM), and was antiproliferative in the CBMN test (CHO-K1: 0.5 to 10 μM; HeLa: 5 and 10 μM). The drug did not induce apoptosis, micronucleus neither altered gene expression. IRN and ISA were genotoxic for HeLa cells (3 and 24 h) at all doses tested. IRN (100 and 150 mg/kg) also induced genotoxicity in vivo (4 h). Conclusion: IRN and ISA have properties that make them candidates as chemotherapeutics for further pharmacological investigations.

Published
2017
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2. Anti-inflammatory activity ofAlchornea triplinerviaethyl acetate fraction: Inhibition of H2O2, NO and TNF-α

Author

Tamara Regina Calvo, Flávia Cristine Mascia Lopes, Wagner Vilegas, and Iracilda Zeppone Carlos

Subjects
Pharmacology, Alchornea triplinervia, biology, Traditional medicine, medicine.drug_class, Ethyl acetate, Euphorbiaceae, Pharmaceutical Science, Context (language use), General Medicine, biology.organism_classification, Anti-inflammatory, Nitric oxide, chemistry.chemical_compound, Complementary and alternative medicine, chemistry, Griess test, Drug Discovery, Botany, medicine, Molecular Medicine, Phenols
Abstract

Context: Alchornea triplinervia (Spreng.) Mull. Arg. (Euphorbiaceae) is a tree widespread in many Brazilian states. This plant naturally occurs in different ecosystems including tropical Atlantic forest, Amazon rain forest, moist tropical mixed forest, savanna forest, among others. Local populations traditionally use it in tea form to treat gastric disturbances.Objective: The objective of this research was to evaluate the plant A. triplinervia as a potential inhibitor of some macrophage functions involved in the inflammatory process.Materials and methods: The effects of Alchornea triplinervia ethyl acetate fraction (AtF) on hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) production in peritoneal macrophages were investigated using phenol red, Griess reagent and a sandwich immunoassay, respectively.Results: AtF chromatographic analyses indicate the presence of flavonoids as majority compounds. The fraction also showed an intense inhibition of H2O2 and NO production. The inhi...

Published
2010
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3. Antimycobacterial activity ofIndigofera suffruticosawith activation potential of the innate immune system

Author

Clarice Queico Fujimura Leite, Iracilda Zeppone Carlos, Wagner Vilegas, Flávia Cristine Mascia Lopes, Camila Bernardes de Andrade Carli, Fernando Rogério Pavan, Rubens Rodrigues dos Santos, Daniele C. G. Maia, Marcela B. Quilles, and Tamara Regina Calvo

Subjects
Cell Survival, medicine.drug_class, Pharmaceutical Science, Microbial Sensitivity Tests, Biology, Antimycobacterial, Microbiology, Mycobacterium tuberculosis, Mice, chemistry.chemical_compound, Immune system, Immunity, Drug Discovery, medicine, Animals, Cells, Cultured, Pharmacology, Innate immune system, Plant Extracts, General Medicine, Meth, Plant Components, Aerial, biology.organism_classification, Immunity, Innate, Indigofera, Anti-Bacterial Agents, Complementary and alternative medicine, chemistry, Indigofera suffruticosa, Molecular Medicine
Abstract

Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The antimycobacterial activity and the innate immune response of methanol (METH) and dichloromethane (DCM) extracts of Indigofera suffruticosa Miller (Fabaceae) were evaluated. We observed that the minimum inhibitory concentrations (MICs) for METH and DCM extracts were 125 and 1000 microg/mL, respectively. However, they were able to induce the innate immune response through the production of high levels of NO and TNF-alpha (p < 0.001) by peritoneal exudate cells (PECs). These results suggest that I. suffruticosa extracts may have an important immunological role in the control of TB once macrophage activity is induced by them.

Published
2010
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4. Anti-inflammatory activity of Alchornea triplinervia ethyl acetate fraction: inhibition of H₂O₂, NO and TNF-α

Author

Flávia Cristine Mascia, Lopes, Tamara Regina, Calvo, Wagner, Vilegas, and Iracilda Zeppone, Carlos

Subjects
Flavonoids, Inflammation, Male, Dose-Response Relationship, Drug, Plant Extracts, Tumor Necrosis Factor-alpha, Anti-Inflammatory Agents, Euphorbiaceae, Hydrogen Peroxide, Nitric Oxide, Plant Leaves, Mice, Phenols, Macrophages, Peritoneal, Animals, Medicine, Traditional, Brazil
Abstract

Alchornea triplinervia (Spreng.) Müll. Arg. (Euphorbiaceae) is a tree widespread in many Brazilian states. This plant naturally occurs in different ecosystems including tropical Atlantic forest, Amazon rain forest, moist tropical mixed forest, savanna forest, among others. Local populations traditionally use it in tea form to treat gastric disturbances.The objective of this research was to evaluate the plant A. triplinervia as a potential inhibitor of some macrophage functions involved in the inflammatory process.The effects of Alchornea triplinervia ethyl acetate fraction (AtF) on hydrogen peroxide (H₂O₂), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) production in peritoneal macrophages were investigated using phenol red, Griess reagent and a sandwich immunoassay, respectively.AtF chromatographic analyses indicate the presence of flavonoids as majority compounds. The fraction also showed an intense inhibition of H₂O₂ and NO production. The inhibitory effects of the fraction in H₂O₂ and NO production ranged from 72.25 ± 4.68 to 69.64 ± 4.21 and from 47.8 ± 8.96 to 76.77 ± 8.11%, respectively in the two tested concentrations, 15.62 and 62.5 μg/mL. TNF-α production was partially inhibited in the tested concentrations and the inhibitory rate was around 18%.It is supposed that the elevated biological potential of A. triplinervia is related to the presence of phenolic compounds in the plant leaves. According to the results observed in this study, it is suggested that AtF presents anti-inflammatory activity, supporting the traditional use of A. triplinervia in Brazilian folk medicine.

Published
2010

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