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Your search keyword '"Tom, Willems"' showing total 3 results
Start Over Author "Tom, Willems" Journal pharmaceutical research
3 results on '"Tom, Willems"'

1. [Untitled]

Author

Remigius U. Agu, Tom Willems, Renaat Kinget, Johan Van Lint, Jackie V. Vandenheede, Sandy Vandoninck, Hoang Vu Dang, Norbert Verbeke, and Mark Jorissen

Subjects
Pharmacology, chemistry.chemical_classification, Organic Chemistry, Pharmaceutical Science, Mucous membrane of nose, Phenylalanine, Transporter, respiratory system, Biology, In vitro, Epithelium, Amino acid, medicine.anatomical_structure, chemistry, Biochemistry, Cell culture, Gene expression, otorhinolaryngologic diseases, medicine, Molecular Medicine, Pharmacology (medical), Biotechnology
Abstract

Purpose. The purpose of this study was to provide functional and molecular evidence to support the existence of large neutral amino acid transporters in human nasal epithelium using nasal primary cell culture model.

Published
2003

2. [Untitled]

Author

Tom Willems, Renaat Kinget, Remigius U. Agu, Guy Van den Mooter, Mark Jorissen, and Patrick Augustijns

Subjects
Pharmacology, Nasal cavity, Cilium, Organic Chemistry, Pharmacology toxicology, Pharmaceutical Science, Biology, In vitro, Epithelium, Cell biology, medicine.anatomical_structure, Cell culture, Immunology, Difenhidramina, medicine, Molecular Medicine, Pharmacology (medical), Ciliary beating, Biotechnology
Abstract

Purpose. To test two in vitro human nasal epithelial cell culture systems for their ability to screen the effects of pharmaceutical compounds on ciliary beating.

Published
1999

3. In vitro polarized transport of L-phenylalanine in human nasal epithelium and partial characterization of the amino acid transporters involved

Author

Remigius, Agu, Hoang Vu, Dang, Mark, Jorissen, Tom, Willems, Sandy, Vandoninck, Johan, Van Lint, Jackie V, Vandenheede, Renaat, Kinget, and Norbert, Verbeke

Subjects
Nasal Mucosa, Amino Acid Transport Systems, Reverse Transcriptase Polymerase Chain Reaction, Phenylalanine, Temperature, Animals, Biological Transport, Active, Humans, RNA, Messenger, Cells, Cultured
Abstract

The purpose of this study was to provide functional and molecular evidence to support the existence of large neutral amino acid transporters in human nasal epithelium using nasal primary cell culture model.L-Phenylalanine was used as a model substrate to characterize carrier-mediated permeation of amino acids across human nasal epithelium. The influence of temperature, concentration, other amino acids, metabolic/transport inhibitors, and polarity/stereo-selectivity on transport of the model compound was investigated. Reverse transcriptase polymerase chain reaction was used for molecular characterization of the existence of the transporters.The transport of L-phenylalanine across the human nasal epithelium was polarized (apical --basolateralbasolateral --apical), saturable (Km = 1.23 mM; Vmax = 805.1 nmol/mg protein/min) and stereo-selective (permeation of L-phenylalanineD-Phenylalanine). Its permeation was significantly (0.05) reduced by cationic, small and large neutral amino acids, oubain, amiloride, sodium-free medium, and temperature lowering. Reverse transcriptase polymerase chain reaction revealed the presence of the broad-scope cationic-dependent amino acid transporter gene (y+LAT-2) in the human nasal epithelium.Based on the results of this study, one may postulate that the human nasal epithelium expresses L-amino acid transporters. More studies are necessary for detailed characterization of the transporters.

Published
2003

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