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Your search keyword '"Biophysical assays"' showing total 3 results
Start Over Descriptor "Biophysical assays" Journal pharmaceutics
3 results on '"Biophysical assays"'

1. Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Author

Olga A. Krasheninina, Evgeny K. Apartsin, Elena Fuentes, Aleksandra Szulc, Maksim Ionov, Alya G. Venyaminova, Dzmitry Shcharbin, F. Javier de la Mata, Maria Bryszewska, and Rafael Gόmez

Subjects
carbosilane, dendrimers, anticancer siRNA, nucleic acids, dendriplexes, complexation, transfection, cytotoxicity, biophysical assays, Pharmacy and materia medica, RS1-441
Abstract

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both “easy-to-transfect” cells (HeLa) and “hard-to transfect” ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.

Published
2019
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2. Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Author

Aleksandra Szulc, Rafael Gόmez, Alya G. Venyaminova, Evgeny K. Apartsin, Maksim Ionov, Elena Fuentes, F. Javier de la Mata, Dzmitry Shcharbin, Maria Bryszewska, and Olga A. Krasheninina

Subjects
Small interfering RNA, complexation, anticancer siRNA, Pharmaceutical Science, lcsh:RS1-441, carbosilane, 02 engineering and technology, 01 natural sciences, Article, dendrimers, biophysical assays, HeLa, lcsh:Pharmacy and materia medica, Dendrimer, Cytotoxicity, biology, 010405 organic chemistry, Chemistry, dendriplexes, Cationic polymerization, Transfection, 021001 nanoscience & nanotechnology, biology.organism_classification, 3. Good health, 0104 chemical sciences, nucleic acids, transfection, Apoptosis, Cancer cell, Biophysics, cytotoxicity, 0210 nano-technology
Abstract

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both &ldquo, easy-to-transfect&rdquo, cells (HeLa) and &ldquo, hard-to transfect&rdquo, ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.

Published
2019

3. Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells.

Author

Krasheninina, Olga A., Apartsin, Evgeny K., Fuentes, Elena, Szulc, Aleksandra, Ionov, Maksim, Venyaminova, Alya G., Shcharbin, Dzmitry, de la Mata, F. Javier, Bryszewska, Maria, and Gόmez, Rafael

Subjects
*DENDRIMERS, *NANOPARTICLES, *SMALL interfering RNA, *HELA cells, *APOPTOSIS
Abstract

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both "easy-to-transfect" cells (HeLa) and "hard-to transfect" ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

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