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As an advanced accelerator concept, laser wakefield acceleration, with its attractive acceleration gradient, has invoked great interest worldwide. The electron beams from laser wakefield acceleration were assumed to have the advantage of “jitterfree” or relatively small jitter, since the emergence of quasimonoenergetic bunch structures were considered to result from the trapping of electrons into the first bucket of the plasma wave. However, the emission times of the electron bunches from laser wakefield acceleration have not been real time monitored in experiments up to now. To examine this, we introduced the electro-optic spatial decoding method. The relative emission times of the electrons were observed to have variations with different plasma densities when using a laser with moderate intensity. The study shows that timing issues should not be ignored in some occasions of laser wakefield acceleration. The method used in this paper could be a candidate serving as a single-shot nondestructive electron bunch timing monitor for laser wakefield experiment and potential ultrafast pump-probe studies.