1. Human transcriptome analysis of acute responses to glucose ingestion reveals the role of leukocytes in hyperglycemia-induced inflammation
- Author
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Hyung Jin Choi, So Young Jung, Hyo Jeong Ban, Hye Sun Yun, Hye Young Nam, Yeonjung Kim, Bok Ghee Han, Jae Pil Jeon, Eun Jung Hong, Hyun Ju Kang, and Seung Eun Jung
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Inflammation ,Biology ,Real-Time Polymerase Chain Reaction ,Transcriptome ,Pathogenesis ,Immunity ,Diabetes mellitus ,Internal medicine ,Gene expression ,Leukocytes ,Genetics ,medicine ,Humans ,Insulin ,Cells, Cultured ,Aged ,Oligonucleotide Array Sequence Analysis ,Regulation of gene expression ,Models, Genetic ,Gene Expression Profiling ,Reproducibility of Results ,Feeding Behavior ,Middle Aged ,medicine.disease ,Glucose ,Endocrinology ,Gene Expression Regulation ,Hyperglycemia ,Immunology ,Female ,medicine.symptom - Abstract
Glucose ingestion-induced hyperglycemia has been known to induce inflammation, which is related to the pathogenesis of diabetic complications. To examine acute gene expression responses to physiological oral glucose ingestion in human circulating leukocytes, we conducted a microarray study of human circulating leukocytes sampled before, 1 h after, and 2 h after glucose ingestion in community-based participants without previous histories of diabetes ( n = 60). Ingestion of 75 g glucose successfully induced acute hyperglycemia (glucose concentration 91.6 ± 5.3 mg/dl for fasting and 180.7 ± 48.5 mg/dl for 1 h after glucose ingestion). Oral glucose ingestion significantly increased the expressions of 23 genes and decreased the expressions of 13 genes [false discovery rate (FDR) P value
- Published
- 2012
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