1. Role of spinal glial cells in excitability of wide dynamic range neurons and the development of neuropathic pain with the L5 spinal nerve transection in the rats: Behavioral and electrophysiological study
- Author
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Laleh Rezaee, Abbas Haghparast, and Homa Manaheji
- Subjects
Male ,Long-Term Potentiation ,Experimental and Cognitive Psychology ,Nerve Fibers, Myelinated ,Propentofylline ,03 medical and health sciences ,Behavioral Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,LTP induction ,Animals ,0501 psychology and cognitive sciences ,050102 behavioral science & comparative psychology ,Rats, Wistar ,Ligation ,Neurons ,Nerve Fibers, Unmyelinated ,Behavior, Animal ,business.industry ,05 social sciences ,Long-term potentiation ,Spinal cord ,Electrophysiological Phenomena ,Rats ,Neuroprotective Agents ,Spinal Nerves ,medicine.anatomical_structure ,Spinal Cord ,nervous system ,chemistry ,Hyperalgesia ,Xanthines ,Spinal nerve ,Synapses ,Neuropathic pain ,Peripheral nerve injury ,Neuralgia ,Cell activation ,business ,Neuroglia ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The activation of glial cells affects the neuronal excitability in the spinal cord. Therefore, in this study, we tried to find out the modulatory role of spinal glial cells in the excitability of wide dynamic range (WDR) neurons, induction of the long-term potentiation (LTP) and development of neuropathic pain by L5 spinal nerve transection model in the rats. Forty-eight adult male Wistar rats were used to measure the paw withdrawal threshold to mechanical stimuli and also, to carry out the spinal extracellular single unit recording experiments. In these experiments, spinal nerve ligation (SNL) and a daily injection of propentofylline (1 mg/kg, ip) as a glial cell inhibitor agent, 1 h following nerve ligation during 7-day post-SNL period, were performed. Our findings showed that the mechanical allodynia, and synaptically-evoked firing were caused LTP in the Aδ-fiber, C-fiber and lesser in the Aβ-fiber after high frequency stimulation. Daily injection of propentofylline considerably decreased LTP induction in the Aδ- and C-fibers (P
- Published
- 2019
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