1. Identification of antioxidant phenolic compounds in feverfew (Tanacetum parthenium) by HPLC-ESI-MS/MS and NMR
- Author
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Guohui Huang, Ronald D. Galyean, Feng Chen, Xi Wang, Yonnie Wu, Meidui Dong, Guoqing He, Lilin He, and Changqing Wu
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Antioxidant ,Magnetic Resonance Spectroscopy ,DPPH ,medicine.medical_treatment ,Hplc esi ms ms ,Plant Science ,Tanacetum parthenium ,Biochemistry ,High-performance liquid chromatography ,Antioxidants ,Analytical Chemistry ,chemistry.chemical_compound ,Phenols ,Liquid chromatography–mass spectrometry ,Drug Discovery ,medicine ,Chromatography, High Pressure Liquid ,Chromatography ,Molecular Structure ,Chemistry ,General Medicine ,Complementary and alternative medicine ,Polyphenol ,Molecular Medicine ,Medicinal herbs ,Food Science - Abstract
Antioxidant polyphenolic acids in the medicinal herb feverfew (Tanacetum parthenium) were isolated through in vitro bioassay-orientated antioxidant tests in response to 1,1-diphenyl-2-picrylhydrazyl (DPPH•) free radical scavenging and Fe2+-chelating activities. Purification of the active compounds and their structural elucidation involved a variety of techniques including open-column chromatography, HPLC, GC-MS, LC-MS and NMR. Major compounds with potent DPPH• scavenging activities were characterised as 3,5-, 4,5- and 3,4-di-O-caffeoylquinic acids (DCQAs). This is the first report of DCQAs found in feverfew. Copyright © 2007 John Wiley & Sons, Ltd.
- Published
- 2007