1. Effects of nutrients on anthocyanin production in cultured cells of Aralia cordata
- Author
-
Kumiko Iida, Tsutomu Furuya, Yoshihisa Asada, Kyoko Hajiro, Kazuo Sakamoto, Kimiko Sawamura, and Takafumi Yoshikawa
- Subjects
chemistry.chemical_classification ,biology ,fungi ,Cyanidin ,food and beverages ,Plant Science ,General Medicine ,Horticulture ,biology.organism_classification ,Biochemistry ,chemistry.chemical_compound ,chemistry ,Auxin ,Anthocyanin ,Callus ,Cytokinin ,Botany ,Araliaceae ,Kinetin ,Aralia cordata ,Molecular Biology - Abstract
Good results for anthocyanin production in dark-grown calli of Aralia cordata Thunb. were obtained on the addition of sucrose as the carbon source and NH4+ and NO3− at a molar ratio of 1:4 (15 mM total nitrogen). Also, by supplementing NAA as auxin and kinetin as cytokinin, good results were obtained. The anthocyanin yield of high-selected callus cultures was 13.0% maximum dry weight on being cultured for three weeks in darkness. The major anthocyanin, about 70% of the total pigment in the cells, was identified as cyanidin 3-xylosylgalactoside.
- Published
- 1993