1. Callose Synthase Family Genes Involved in the Grapevine Defense Response to Downy Mildew Disease
- Author
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Jiang Lu, Shufang Fu, Li Jiao, Yali Zhang, Ying Yu, and Ling Yin
- Subjects
0106 biological sciences ,0301 basic medicine ,Plant Science ,01 natural sciences ,Gene Expression Regulation, Enzymologic ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Botany ,Plant defense against herbivory ,Vitis ,Gene ,Pathogen ,Glucans ,Phylogeny ,Plant Diseases ,Oomycete ,biology ,Callose ,food and beverages ,Promoter ,biology.organism_classification ,Plant Leaves ,030104 developmental biology ,chemistry ,Oomycetes ,Glucosyltransferases ,Plasmopara viticola ,Downy mildew ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
The deposition of callose is a common plant defense response to intruding pathogens and part of the plant’s innate immunity. In this study, eight grapevine callose synthase (CalS) genes were identified and characterized. To investigate biological function of CalS in grapevine against the infection of Plasmopara viticola, expression patterns of grapevine CalS family genes were analyzed among resistant/susceptible cultivars. After P. viticola infection, expression of CalS1, 3, 7, 8, 9, 10, and 11 were significantly modified among the grapevine cultivars. For example, the expression of CalS1 and CalS10 were greatly increased in downy mildew (DM)-immune Muscadinia rotundifolia ‘Carlos’ and ‘Noble’. Transient expression assay with promoters of the CalS1 and CalS10 genes confirmed that they were regulated by the oomycete pathogen P. viticola. CalS1 promoter activity was also significantly up-regulated by ABA in DM-immune M. rotundifolia ‘Noble’, but down-regulated in DM-susceptible Vitis vinifera ‘Chardonnay’. The CalS1 promoter, however, was also down-regulated by GA in ‘Chardonnay’, but not affected in ‘Noble’. The promoter activity of CalS10 was significantly up-regulated by GA in ‘Chardonnay’, but not regulated by ABA at all. It is proposed that CalS1 and CalS10 were involved in grapevine defense against DM disease.
- Published
- 2015