1. Application of fat- and water-soluble component trace detection in filial generation selection in soybean [ Glycine max (L.) Merr.] seeds using high-performance liquid chromatography.
- Author
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Wang, Shaodong, Wang, Hao, Kanamaru, Kyohei, Liu, Wei, Jiang, Yan, and Li, Yuan‐ming
- Subjects
SOYBEAN varieties ,HIGH performance liquid chromatography ,PLANT extracts ,SEEDS ,PLANT vitamins ,REGRESSION analysis ,CAROTENES ,OIL & fat content of plants - Abstract
With 2 figures and 2 tables Abstract An extraction and detection method was reported for fat- and water-soluble components in primary soybean seed. The HPLC retention times were 10 min for lutein, 23 min for δ-tocopherol, 27 min for γ-tocopherol, 30 min for α-tocopherol, 33 min for α-carotene and 39 min for β-carotene. The retention times of the isoflavones were 7 min for daidzin, 11 min for genistin, 15 min for malonyldaidzin and 20 min for malonylgenistin. Linear regression and two-way anova analysis were performed, and the test results indicated that there was a significant correlation ( r
2 = 0.9973, P < 0.0001) and no significant difference (P = 0.8862 > 0.05) between the traditional method and the proposed method. Moreover, the strain '07-27', which has high α-tocopherol and isoflavone contents in the F2 : 3 lines (F2 -derived lines in F3 ), was screened and then crossed between a high-isoflavone soybean variety, 'Toiku 241', and a high-α-tocopherol soybean variety, 'Keszthelyi A.S.' (pollen parent). The contents of α-tocopherol and total isoflavones were 8.91 and 622.96 mg/100 g DW, respectively. [ABSTRACT FROM AUTHOR]- Published
- 2012
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