1. The Cell Fate Controlling CLE40 Peptide Requires CNGCs to Trigger Highly Localized Ca2+ Transients in Arabidopsis thaliana Root Meristems.
- Author
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Breiden M, Olsson V, Blümke P, Schlegel J, Gustavo-Pinto K, Dietrich P, Butenko MA, and Simon R
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Cell Differentiation genetics, Cyclic Nucleotide-Gated Cation Channels genetics, Genetic Variation, Genotype, Meristem genetics, Plant Roots genetics, Signal Transduction drug effects, Signal Transduction genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Calcium metabolism, Cell Differentiation drug effects, Cyclic Nucleotide-Gated Cation Channels metabolism, Meristem metabolism, Plant Roots metabolism
- Abstract
Communication between plant cells and their biotic environment largely depends on the function of plasma membrane localized receptor-like kinases (RLKs). Major players in this communication within root meristems are secreted peptides, including CLAVATA3/EMBRYO SURROUNDING REGION40 (CLE40). In the distal root meristem, CLE40 acts through the RLK ARABIDOPSIS CRINKLY4 (ACR4) and the leucine-rich repeat (LRR) RLK CLAVATA1 (CLV1) to promote cell differentiation. In the proximal meristem, CLE40 signaling requires the LRR receptor-like protein CLAVATA2 (CLV2) and the membrane localized pseudokinase CORYNE (CRN) and serves to inhibit cell differentiation. The molecular components that act immediately downstream of the CLE40-activated receptors are not yet known. Here, we show that active CLE40 signaling triggers the release of intracellular Ca2+ leading to increased cytosolic Ca2+ concentration ([Ca2+]cyt) in a small subset of proximal root meristem cells. This rise in [Ca2+]cyt depends on the CYCLIC NUCLEOTIDE GATED CHANNELS (CNGCs) 6 and 9 and on CLV1. The precise function of changes in [Ca2+]cyt is not yet known but might form a central part of a fine-tuned response to CLE40 peptide that serves to integrate root meristem growth with stem cell fate decisions and initiation of lateral root primordia., (© The Author(s) 2021. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2021
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