1. Cell Wall Hemicellulose Contributes Significantly to Aluminum Adsorption and Root Growth in Arabidopsis.
- Author
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Jian Li Yang, Xiao Fang Zhu, You Xiang Peng, Cheng Zheng, Gui Xin Li, Yu Liu, Yuan Zhi Shi, and Shao Jian Zheng
- Subjects
PLANT cell walls ,HEMICELLULOSE ,ALUMINUM ,PLANT roots ,ARABIDOPSIS thaliana - Abstract
The cell wall (CW) has been recognized as the major target of aluminum (Al) toxicity. However, the components responsible for Al accumulation and the mechanisms of Al-induced CW function disruption are still elusive. The contribution of different CW components (pectin, hemicellulose 1 [HC1], and HC2) to adsorb AI and the effect of A1 on xyloglucan endotransglucosylase/ hydrolyase activity were investigated in Arabidopsis (Arabidopsis thaliana) in this study. A fractionation procedure was optimized to effectively extract different CW components, especially to prevent the HC fraction from pectin contamination. When CW materials extracted from Al-treated roots (50 µM Al for 24 h) were fractionated, about 75% of CW Al accumulated in the HCl fraction. A time-dependent kinetic study showed that only when the HCl fraction was removed was the amount of Al adsorbed decreased sharply. In vivo localization of xyloglucan endotransglucosylase (XET) activity showed that AI greatly inhibited this enzyme activity within 30 min of exposure, which was concomitant with Al-induced callose deposition in roots. Results from real-time reverse transcription-polymerase chain reaction indicated that three genes may constitute the major contributors to XET activity and that the inhibition of XET activity by Al is caused by transcriptional regulation. These results, to our knowledge for the first time, demonstrate that HC is the major pool for Al accumulation. Furthermore, Al-induced reduction in XET activity could play an important role in Al-induced root growth inhibition. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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