15 results on '"Hui, Liu"'
Search Results
2. Parasite dodder enables transfer of bidirectional systemic nitrogen signals between host plants
- Author
-
Guojing Shen, Yuxing Xu, Huifu Zhuang, Jing Xie, Hui Liu, Jianqiang Wu, and Jingxiong Zhang
- Subjects
0106 biological sciences ,Physiology ,PII Nitrogen Regulatory Proteins ,Plant Science ,Genes, Plant ,01 natural sciences ,Host-Parasite Interactions ,Transcriptome ,03 medical and health sciences ,Focus Issue on Parasitic Plants ,Gene Expression Regulation, Plant ,Botany ,Genetics ,Parasite hosting ,Host plants ,Gene ,030304 developmental biology ,0303 health sciences ,biology ,fungi ,food and beverages ,Cuscuta ,Cuscuta campestris ,biology.organism_classification ,Convolvulaceae ,Cucumis ,Signal Transduction ,010606 plant biology & botany - Abstract
Dodder (Cuscuta spp., Convolvulaceae) is a genus of parasitic plants with worldwide distribution. Dodders are able to simultaneously parasitize two or more adjacent hosts, forming dodder-connected plant clusters. Nitrogen (N) deficiency is a common challenge to plants. To date, it has been unclear whether dodder transfers N-systemic signals between hosts grown in N-heterogeneous soil. Transcriptome and methylome analyses were carried out to investigate whether dodder (Cuscuta campestris) transfers N-systemic signals between N-replete and N-depleted cucumber (Cucumis sativus) hosts, and it was found that N-systemic signals from the N-deficient cucumber plants were rapidly translocated through C. campestris to the N-replete cucumber plants. Unexpectedly, certain systemic signals were also transferred from the N-replete to N-depleted cucumber hosts. We demonstrate that these systemic signals are able to regulate large transcriptome and DNA methylome changes in the recipient hosts. Importantly, N stress also induced many long-distance mobile mRNA transfers between C. campestris and hosts, and the bilateral N-systemic signaling between N-replete and N-depleted hosts had a strong impact on the inter-plant mobile mRNAs. Our 15N labeling experiment indicated that under N-heterogeneous conditions, N-systemic signals from the N-deficient cucumber hosts did not obviously change the N-uptake activity of the N-replete cucumber hosts; however, in plant clusters comprising C. campestris-connected cucumber and soybean (Glycine max) plants, if the soybean plants were N-starved, the cucumber plants exhibited increased N-uptake activity. This study reveals that C. campestris facilitates plant–plant communications under N-stress conditions by enabling extensive bilateral N-systemic signaling between different hosts.
- Published
- 2020
3. WRINKLED1 Regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING Proteins that Inhibit Fatty Acid Synthesis
- Author
-
Zhiyang Zhai, Kate Kuczynski, John Shanklin, Hui Liu, Jörg Schwender, and Jantana Keereetaweep
- Subjects
0106 biological sciences ,Physiology ,Protein domain ,Arabidopsis ,Biotin ,Plant Science ,01 natural sciences ,Conserved sequence ,chemistry.chemical_compound ,Protein Domains ,Genetics ,Promoter Regions, Genetic ,News and Views ,Conserved Sequence ,Research Articles ,Fatty acid synthesis ,biology ,Arabidopsis Proteins ,Fatty Acids ,Wild type ,Promoter ,biology.organism_classification ,Lipids ,Pyruvate carboxylase ,Cell biology ,chemistry ,Seedlings ,Acetyl-CoA Carboxylase ,Transcription Factors ,010606 plant biology & botany - Abstract
WRINKLED1 (WRI1) is a transcriptional activator that binds to a conserved sequence (designated as AW box) boxes in the promoters of many genes from central metabolism and fatty acid (FA) synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins lack a biotin-attachment domain and are therefore inactive, but in the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE, and consequently FA synthesis. Here, we tested the interaction of WRI1 with BADC genes in Arabidopsis (Arabidopsis thaliana) and found purified WRI1 bound with high affinity to canonical AW boxes from the promoters of all three BADC genes. Consistent with this observation, both expression of BADC1, BADC2, and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to the wild type, and elevated upon WRI1 overexpression. The double mutant badc1 badc2 phenocopied wri1-1 with respect to both reduction in root length and elevation of indole-3-acetic acid-Asp levels relative to the wild type. Overexpression of BADC1 in wri1-1 decreased indole-3-acetic acid-Asp content and partially rescued its short-root phenotype, demonstrating a role for BADCs in seedling establishment. That WRI1 positively regulates genes encoding both FA synthesis and BADC proteins (i.e. conditional inhibitors of FA synthesis), represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the transcription of their FA synthetic capacity with their capacity to biochemically downregulate it.
- Published
- 2019
4. Biotin Attachment Domain-Containing Proteins Irreversibly Inhibit Acetyl CoA Carboxylase
- Author
-
Jantana Keereetaweep, Hui Liu, Zhiyang Zhai, and John Shanklin
- Subjects
0106 biological sciences ,0301 basic medicine ,chemistry.chemical_classification ,Physiology ,Chemistry ,Protein subunit ,Mutant ,Acetyl-CoA carboxylase ,Plant Science ,01 natural sciences ,Pyruvate carboxylase ,03 medical and health sciences ,chemistry.chemical_compound ,Oleic acid ,030104 developmental biology ,Enzyme ,Biotin ,Biochemistry ,Genetics ,Fatty acid synthesis ,010606 plant biology & botany - Abstract
The first committed step in fatty acid synthesis is mediated by acetyl-CoA carboxylase (ACCase), a biotin-dependent enzyme that carboxylates acetyl-CoA to produce malonyl-CoA. ACCase can be feedback regulated by short-term or long-term exposure to fatty acids in the form of Tween 80 (predominantly containing oleic acid), which results in reversible or irreversible ACCase inhibition, respectively. Biotin attachment domain-containing (BADC) proteins are inactive analogs of biotin carboxyl transfer proteins that lack biotin, and their incorporation into ACCase down-regulates its activity by displacing active (biotin-containing) biotin carboxyltransferase protein subunits. Arabidopsis (Arabidopsis thaliana) lines containing T-DNA insertions in BADC1, BADC2, and BADC3 were used to generate badc1 badc2 and badc1 badc3 double mutants. The badc1 badc3 mutant exhibited normal growth and development; however, ACCase activity was 26% higher in badc1 badc3 and its seeds contained 30.1% more fatty acids and 32.6% more triacylgycerol relative to wild-type plants. To assess whether BADC contributes to the irreversible phase of ACCase inhibition, cell suspension cultures were generated from the leaves of badc1 badc3 and wild-type plants and treated with 10 mm Tween 80. Reversible ACCase inhibition was similar in badc1 badc3 and wild-type cultures after 2 d of Tween 80 treatment, but irreversible inhibition was reduced by 50% in badc1 badc3 relative to wild-type plants following 4 d of Tween 80 treatment. In this study, we present evidence for two important homeostatic roles for BADC proteins in down-regulating ACCase activity: by acting during normal growth and development and by contributing to its long-term irreversible feedback inhibition resulting from the oversupply of fatty acids.
- Published
- 2018
5. Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation
- Author
-
Changcheng Xu, Hui Liu, Zhiyang Zhai, and John Shanklin
- Subjects
0106 biological sciences ,0301 basic medicine ,Sucrose ,Physiology ,Mutant ,Arabidopsis ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Dry weight ,Lipid biosynthesis ,Genetics ,Diacylglycerol O-Acyltransferase ,Sugar ,Triglycerides ,Plant Proteins ,chemistry.chemical_classification ,Arabidopsis Proteins ,Fatty Acids ,Wild type ,food and beverages ,Membrane Transport Proteins ,Fatty acid ,Articles ,Lipid Metabolism ,Plant Leaves ,030104 developmental biology ,chemistry ,Biochemistry ,Seedlings ,Mutation ,Phloem ,Sugars ,Transcription Factors ,010606 plant biology & botany - Abstract
Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1 mutation disables the small subunit of ADP-glucose pyrophosphorylase, the first step in starch synthesis, and the suc2 mutation disables a sucrose/proton symporter that facilitates sucrose loading from leaves into phloem. The adg1suc2 double mutant increases glucose plus sucrose content in leaves 80-fold relative to the wild type, total fatty acid (FA) content 1.8-fold to 8.3% dry weight, and TAG more than 10-fold to 1.2% dry weight. The WRINKLED1 transcription factor also accumulates to higher levels in these leaves, and the rate of FA synthesis increases by 58%. Adding tt4, which disables chalcone synthase, had little effect, but adding the tgd1 mutation, which disables an importer of lipids into plastids to create adg1suc2tt4tgd1, increased total leaf FA to 13.5% dry weight and TAG to 3.8% dry weight, demonstrating a synergistic effect upon combining these mutations. Combining adg1suc2 with the sdp1 mutation, deficient in the predominant TAG lipase, had little effect on total FA content but increased the TAG accumulation by 66% to 2% dry weight. Expression of the WRINKLED1 transcription factor, along with DIACYLGLYCEROL ACYLTRANSFERASE1 and the OLEOSIN1 oil body-associated protein, in the adg1suc2 mutant doubled leaf FA content and increased TAG content to 2.3% dry weight, a level 4.6-fold higher than that resulting from expression of the same factors in the wild type.
- Published
- 2017
6. Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice
- Author
-
Xue-Hui Liu, Yanbao Tian, Yaping Gao, Baocai Zhang, Congwu He, Xiangling Liu, Zuopeng Xu, Markus Pauly, Dongmei Zhang, Shanshan Zang, and Yihua Zhou
- Subjects
0106 biological sciences ,0301 basic medicine ,Physiology ,Mutant ,Golgi Apparatus ,Plant Science ,Genetically modified crops ,Biology ,01 natural sciences ,Cell wall ,03 medical and health sciences ,Acetyltransferases ,Gene Expression Regulation, Plant ,Genetics ,Phylogeny ,Plant Diseases ,Plant Proteins ,Birefringence ,Oryza sativa ,food and beverages ,Acetylation ,Oryza ,Trichomes ,Articles ,Plants, Genetically Modified ,Xylan ,Trichome ,Xylan acetylation ,Plant Leaves ,030104 developmental biology ,Biochemistry ,Mutation ,Xylans ,010606 plant biology & botany - Abstract
Acetylation is a ubiquitous modification on cell wall polymers, which play a structural role in plant growth and stress defenses. However, the mechanisms for how crop plants accomplish cell wall polymer O-acetylation are largely unknown. Here, we report on the isolation and characterization of two trichome birefringence-like (tbl) mutants in rice (Oryza sativa), which are affected in xylan O-acetylation. ostbl1 and ostbl2 single mutant and the tbl1 tbl2 double mutant displayed a stunted growth phenotype with varied degree of dwarfism. As shown by chemical assays, the wall acetylation level is affected in the mutants and the knock-down and overexpression transgenic plants. Furthermore, NMR spectroscopy analyses showed that all those mutants have varied decreases in xylan monoacetylation. The divergent expression levels of OsTBL1 and OsTBL2 explained the chemotype difference and indicated that OsTBL1 is a functionally dominant gene. OsTBL1 was found to be Golgi-localized. The recombinant OsTBL1 protein incorporates acetyl groups onto xylan. By using xylopentaose, a preferred acceptor substrate, OsTBL1 can transfer up to four acetyl residues onto xylopentaose, and this activity showed saturable kinetics. 2D-NMR spectroscopy showed that OsTBL1 transfers acetate to both 2-O and 3-O sites of xylosyl residues. In addition, ostbl1 and tbl1 tbl2 displayed susceptibility to rice blight disease, indicating that this xylan modification is required for pathogen resistance. This study identifies the major genes responsible for xylan acetylation in rice plants.
- Published
- 2016
7. Parasite dodder enables transfer of bidirectional systemic nitrogen signals between host plants.
- Author
-
Jingxiong Zhang, Yuxing Xu, Jing Xie, Huifu Zhuang, Hui Liu, Guojing Shen, and Jianqiang Wu
- Published
- 2021
- Full Text
- View/download PDF
8. Biotin attachment domain-containing proteins mediate hydroxy fatty acid-dependent inhibition of acetyl CoA carboxylase.
- Author
-
Xiao-Hong Yu, Yuanheng Cai, Keereetaweep, Jantana, Kenneth Wei, Jin Chai, Elen Deng, Hui Liu, and Shanklin, John
- Published
- 2021
- Full Text
- View/download PDF
9. WRINKLED1 Regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING Proteins that Inhibit Fatty Acid Synthesis.
- Author
-
Hui Liu, Zhiyang Zhai, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, and Shanklin, John
- Abstract
WRINKLED1 (WRI1) is a transcriptional activator that binds to a conserved sequence (designated as AW box) boxes in the promoters of many genes from central metabolism and fatty acid (FA) synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins lack a biotin-attachment domain and are therefore inactive, but in the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE, and consequently FA synthesis. Here, we tested the interaction of WRI1 with BADC genes in Arabidopsis (Arabidopsis thaliana) and found purified WRI1 bound with high affinity to canonical AW boxes from the promoters of all three BADC genes. Consistent with this observation, both expression of BADC1, BADC2, and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to the wild type, and elevated upon WRI1 overexpression. The double mutant badc1 badc2 phenocopied wri1-1 with respect to both reduction in root length and elevation of indole-3-acetic acid-Asp levels relative to the wild type. Overexpression of BADC1 in wri1-1 decreased indole-3-acetic acid-Asp content and partially rescued its short-root phenotype, demonstrating a role for BADCs in seedling establishment. That WRI1 positively regulates genes encoding both FA synthesis and BADC proteins (i.e. conditional inhibitors of FA synthesis), represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the transcription of their FA synthetic capacity with their capacity to biochemically downregulate it. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
10. Dynamic Nucleotide-Binding Site and Leucine-Rich Repeat-Encoding Genes in the Grass Family
- Author
-
Hui Liu, Kunpeng Li, Jiongjiong Chen, Yu Zhang, Sha Luo, Wen Wang, Hanhui Kuang, Chen Lu, and Qun Hu
- Subjects
Comparative genomics ,Genetics ,biology ,Physiology ,Sequence analysis ,Pseudogene ,food and beverages ,Plant Science ,R gene ,biology.organism_classification ,Genome ,Conserved sequence ,Brachypodium distachyon ,Gene - Abstract
The proper use of resistance genes (R genes) requires a comprehensive understanding of their genomics and evolution. We analyzed genes encoding nucleotide-binding sites and leucine-rich repeats in the genomes of rice (Oryza sativa), maize (Zea mays), sorghum (Sorghum bicolor), and Brachypodium distachyon. Frequent deletions and translocations of R genes generated prevalent presence/absence polymorphism between different accessions/species. The deletions were caused by unequal crossover, homologous repair, nonhomologous repair, or other unknown mechanisms. R gene loci identified from different genomes were mapped onto the chromosomes of rice cv Nipponbare using comparative genomics, resulting in an integrated map of 495 R loci. Sequence analysis of R genes from the partially sequenced genomes of an African rice cultivar and 10 wild accessions suggested that there are many additional R gene lineages in the AA genome of Oryza. The R genes with chimeric structures (termed type I R genes) are diverse in different rice accessions but only account for 5.8% of all R genes in the Nipponbare genome. In contrast, the vast majority of R genes in the rice genome are type II R genes, which are highly conserved in different accessions. Surprisingly, pseudogene-causing mutations in some type II lineages are often conserved, indicating that their conservations were not due to their functions. Functional R genes cloned from rice so far have more type II R genes than type I R genes, but type I R genes are predicted to contribute considerable diversity in wild species. Type I R genes tend to reduce the microsynteny of their flanking regions significantly more than type II R genes, and their flanking regions have slightly but significantly lower G/C content than those of type II R genes.
- Published
- 2012
11. Biotin Attachment Domain-Containing Proteins Irreversibly Inhibit Acetyl CoA Carboxylase.
- Author
-
Keereetaweep, Jantana, Hui Liu, Zhiyang Zhai, and Shanklin, John
- Abstract
The first committed step in fatty acid synthesis is mediated by acetyl-CoA carboxylase (ACCase), a biotin-dependent enzyme that carboxylates acetyl-CoA to produce malonyl-CoA. ACCase can be feedback regulated by short-term or long-term exposure to fatty acids in the form of Tween 80 (predominantly containing oleic acid), which results in reversible or irreversible ACCase inhibition, respectively. Biotin attachment domain-containing (BADC) proteins are inactive analogs of biotin carboxyl transfer proteins that lack biotin, and their incorporation into ACCase down-regulates its activity by displacing active (biotin-containing) biotin carboxyltransferase protein subunits. Arabidopsis (Arabidopsis thaliana) lines containing T-DNA insertions in BADC1, BADC2, and BADC3 were used to generate badc1 badc2 and badc1 badc3 double mutants. The badc1 badc3 mutant exhibited normal growth and development; however, ACCase activity was 26% higher in badc1 badc3 and its seeds contained 30.1% more fatty acids and 32.6% more triacylgycerol relative to wild-type plants. To assess whether BADC contributes to the irreversible phase of ACCase inhibition, cell suspension cultures were generated from the leaves of badc1 badc3 and wild-type plants and treated with 10 mm Tween 80. Reversible ACCase inhibition was similar in badc1 badc3 and wild-type cultures after 2 d of Tween 80 treatment, but irreversible inhibition was reduced by 50% in badc1 badc3 relative to wild-type plants following 4 d of Tween 80 treatment. In this study, we present evidence for two important homeostatic roles for BADC proteins in down-regulating ACCase activity: by acting during normal growth and development and by contributing to its long-term irreversible feedback inhibition resulting from the oversupply of fatty acids. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
12. Thioredoxin-Mediated ROS Homeostasis Explains Natural Variation in Plant Regeneration.
- Author
-
Hui Zhang, Ting Ting Zhang, Hui Liu, De Ying Shi, Meng Wang, Xiao Min Bie, Xing Guo Li, and Xian Sheng Zhang
- Published
- 2018
- Full Text
- View/download PDF
13. Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation.
- Author
-
Zhiyang Zhai, Hui Liu, Changcheng Xu, and Shanklin, John
- Abstract
Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1 mutation disables the small subunit of ADP-glucose pyrophosphorylase, the first step in starch synthesis, and the suc2 mutation disables a sucrose/proton symporter that facilitates sucrose loading from leaves into phloem. The adg1suc2 double mutant increases glucose plus sucrose content in leaves 80-fold relative to the wild type, total fatty acid (FA) content 1.8-fold to 8.3% dry weight, and TAG more than 10-fold to 1.2% dry weight. The WRINKLED1 transcription factor also accumulates to higher levels in these leaves, and the rate of FA synthesis increases by 58%. Adding tt4, which disables chalcone synthase, had little effect, but adding the tgd1 mutation, which disables an importer of lipids into plastids to create adg1suc2tt4tgd1, increased total leaf FA to 13.5% dry weight and TAG to 3.8% dry weight, demonstrating a synergistic effect upon combining these mutations. Combining adg1suc2 with the sdp1 mutation, deficient in the predominant TAG lipase, had little effect on total FA content but increased the TAG accumulation by 66% to 2% dry weight. Expression of the WRINKLED1 transcription factor, along with DIACYLGLYCEROL ACYLTRANSFERASE1 and the OLEOSIN1 oil body-associated protein, in the adg1suc2 mutant doubled leaf FA content and increased TAG content to 2.3% dry weight, a level 4.6-fold higher than that resulting from expression of the same factors in the wild type. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
14. Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice.
- Author
-
Yaping Gao, Congwu He, Dongmei Zhang, Xiangling Liu, Zuopeng Xu, Yanbao Tian, Xue-Hui Liu, Shanshan Zang, Pauly, Markus, Yihua Zhou, and Baocai Zhang
- Published
- 2017
- Full Text
- View/download PDF
15. Dynamic Nucleotide-Binding Site and Leucine-Rich Repeat-Encoding Genes in the Grass Family.
- Author
-
Sha Luo, Yu Zhang, Qun Hu, Jiongjiong Chen, Kunpeng Li, Chen Lu, Hui Liu, Wen Wang, and Hanhui Kuang
- Subjects
GENES ,GENOMES ,GENETICS ,RICE ,SORGHUM - Abstract
The proper use of resistance genes (R genes) requires a comprehensive understanding of their genomics and evolution. We analyzed genes encoding nucleotide-binding sites and leucine-rich repeats in the genomes of rice (Oryza sativa), maize (Zea mays), sorghum (Sorghum bicolor), and Brachypodium distachyon. Frequent deletions and translocations of R genes generated prevalent presence/absence polymorphism between different accessions/species. The deletions were caused by unequal crossover, homologous repair, nonhomologous repair, or other unknown mechanisms. R gene loci identified from different genomes were mapped onto the chromosomes of rice cv Nipponbare using comparative genomics, resulting in an integrated map of 495 R loci. Sequence analysis of R genes from the partially sequenced genomes of an African rice cultivar and 10 wild accessions suggested that there are many additional R gene lineages in the AA genome of Oryza. The R genes with chimeric structures (termed type I R genes) are diverse in different rice accessions but only account for 5.8% of all R genes in the Nipponbare genome. In contrast, the vast majority of R genes in the rice genome are type II R genes, which are highly conserved in different accessions. Surprisingly, pseudogene-causing mutations in some type II lineages are often conserved, indicating that their conservations were not due to their functions. Functional R genes cloned from rice so far have more type II R genes than type I R genes, but type I R genes are predicted to contribute considerable diversity in wild species. Type I R genes tend to reduce the microsynteny of their flanking regions significantly more than type II R genes, and their flanking regions have slightly but significantly lower G/C content than those of type II R genes. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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