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Start Over Author "Huibo Ren" Journal plant physiology
4 results on '"Huibo Ren"'

1. SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape

Author

Deshu Lin, Huibo Ren, Xiaowei Gao, Dingquan Huang, Yanqiu Yang, Mengting Liu, and Xie Dang

Subjects
0301 basic medicine, Time Factors, Physiology, Arabidopsis, Plant Science, GTPase, Flowers, Biology, 03 medical and health sciences, GTP-Binding Proteins, Gene Expression Regulation, Plant, Botany, Genetics, Arabidopsis thaliana, Monomeric GTP-Binding Proteins, Regulation of gene expression, Microscopy, Confocal, Cell growth, Arabidopsis Proteins, Reverse Transcriptase Polymerase Chain Reaction, fungi, Gene Expression Regulation, Developmental, Articles, biology.organism_classification, Plants, Genetically Modified, eye diseases, Cell biology, 030104 developmental biology, Phenotype, Mutation, Petal, RNA Interference, Guanine nucleotide exchange factor, Cortical microtubule, Signal Transduction
Abstract

Plant organ growth and final shape rely on cell proliferation and, particularly, on cell expansion that largely determines the visible growth of plant organs. Arabidopsis (Arabidopsis thaliana) petals serve as an excellent model for dissecting the coordinated regulation of patterns of cell expansion and organ growth, but the molecular signaling mechanisms underlying this regulation remain largely unknown. Here, we demonstrate that during the late petal development stages, SPIKE1 (SPK1), encoding a guanine nucleotide exchange factor, activates Rho of Plants (ROP) GTPase proteins (ROP2, ROP4, and ROP6) to affect anisotropic expansion of epidermal cells in both petal blades and claws, thereby affecting anisotropic growth of the petal and the final characteristic organ shape. The petals of SPK1 knockdown mutants were significantly longer but narrower than those of the wild type, associated with increased anisotropic expansion of epidermal cells at late development stages. In addition, ROP2, ROP4, and ROP6 are activated by SPK1 to promote the isotropic organization of cortical microtubule arrays and thus inhibit anisotropic growth in the petal. Both knockdown of SPK1 and multiple rop mutants caused highly ordered cortical microtubule arrays that were transversely oriented relative to the axis of cell elongation after development stage 11. Taken together, our results suggest a SPK1-ROP-dependent signaling module that influences anisotropic growth in the petal and defines the final organ shape.

Published
2016

2. Purification and characterization of ZmRIP1, a novel reductant-inhibited protein tyrosine phosphatase from maize

Author

Huang Conglin, Yu Han, Yuanhua Wang, Liyan Liang, Haifeng Jia, Mingzhu Sun, Lin Chen, Huibo Ren, Wensuo Jia, Yanxia Zhao, Bingbing Li, Wu Zhongyi, and Yu Xing

Subjects
Chloroplasts, Physiology, Molecular Sequence Data, Arabidopsis, Plant Science, Protein tyrosine phosphatase, Biology, Zea mays, Dithiothreitol, Arsenicals, Gene Expression Regulation, Enzymologic, Enzyme activator, chemistry.chemical_compound, Gene Expression Regulation, Plant, Genetics, Point Mutation, Amino Acid Sequence, Cysteine, Cloning, Molecular, Cysteine metabolism, Glutathione Transferase, Plant Proteins, chemistry.chemical_classification, Cell Nucleus, Protoplasts, biology.organism_classification, Plants, Genetically Modified, Enzyme Activation, Protein Transport, Enzyme, chemistry, Biochemistry, Cell Biology and Signal Transduction, Electrophoresis, Polyacrylamide Gel, Signal transduction, Protein Tyrosine Phosphatases, Oxidation-Reduction, Signal Transduction
Abstract

Protein tyrosine phosphatases (PTPases) have long been thought to be activated by reductants and deactivated by oxidants, owing to the presence of a crucial sulfhydryl group in their catalytic centers. In this article, we report the purification and characterization of Reductant-Inhibited PTPase1 (ZmRIP1) from maize (Zea mays) coleoptiles, and show that this PTPase has a unique mode of redox regulation and signaling. Surprisingly, ZmRIP1 was found to be deactivated by a reductant. A cysteine (Cys) residue (Cys-181) near the active center was found to regulate this unique mode of redox regulation, as mutation of Cys-181 to arginine-181 allowed ZmRIP1 to be activated by a reductant. In response to oxidant treatment, ZmRIP1 was translocated from the chloroplast to the nucleus. Expression of ZmRIP1 in Arabidopsis (Arabidopsis thaliana) plants and maize protoplasts altered the expression of genes encoding enzymes involved in antioxidant catabolism, such as At1g02950, which encodes a glutathione transferase. Thus, the novel PTPase identified in this study is predicted to function in redox signaling in maize.

Published
2012

3. SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape.

Author

Huibo Ren, Xie Dang, Yanqiu Yang, Dingquan Huang, Mengting Liu, Xiaowei Gao, and Deshu Lin

Abstract

Plant organ growth and final shape rely on cell proliferation and, particularly, on cell expansion that largely determines the visible growth of plant organs. Arabidopsis (Arabidopsis thaliana) petals serve as an excellent model for dissecting the coordinated regulation of patterns of cell expansion and organ growth, but the molecular signaling mechanisms underlying this regulation remain largely unknown. Here, we demonstrate that during the late petal development stages, SPIKE1 (SPK1), encoding a guanine nucleotide exchange factor, activates Rho of Plants (ROP) GTPase proteins (ROP2, ROP4, and ROP6) to affect anisotropic expansion of epidermal cells in both petal blades and claws, thereby affecting anisotropic growth of the petal and the final characteristic organ shape. The petals of SPK1 knockdown mutants were significantly longer but narrower than those of the wild type, associated with increased anisotropic expansion of epidermal cells at late development stages. In addition, ROP2, ROP4, and ROP6 are activated by SPK1 to promote the isotropic organization of cortical microtubule arrays and thus inhibit anisotropic growth in the petal. Both knockdown of SPK1 and multiple rop mutants caused highly ordered cortical microtubule arrays that were transversely oriented relative to the axis of cell elongation after development stage 11. Taken together, our results suggest a SPK1-ROP-dependent signaling module that influences anisotropic growth in the petal and defines the final organ shape. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
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4. Purification and Characterization of ZmRIP1, a Novel Reductant-Inhibited Protein Tyrosine Phosphatase from Maize.

Author

Bingbing Li, Yanxia Zhao, Liyan Liang, Huibo Ren, Yu Xing, Lin Chen, Mingzhu Sun, Yuanhua Wang, Haifeng Jia, Conglin Huang, Zhongyi Wu, and Wensuo Jia

Subjects
PROTEIN-tyrosine phosphatase, CORN proteins, COLEOPTILES, CYSTEINE, GLUTATHIONE transferase, OXIDATION-reduction reaction
Abstract

Protein tyrosine phosphatases (PTPases) have long been thought to be activated by reductants and deactivated by oxidants, owing to the presence of a crucial sulfhydryl group in their catalytic centers. In this article, we report the purification and characterization of Reductant-Inhibited PTPase1 (ZmRIP1) from maize (Zea mays) coleoptiles, and show that this PTPase has a unique mode of redox regulation and signaling. Surprisingly, ZmRIP1 was found to be deactivated by a reductant. A cysteine (Cys) residue (Cys-181) near the active center was found to regulate this unique mode of redox regulation, as mutation of Cys-181 to arginine-181 allowed ZmRIP1 to be activated by a reductant. In response to oxidant treatment, ZmRIP1 was translocated from the chloroplast to the nucleus. Expression of ZmRIP1 in Arabidopsis (Arabidopsis thaliana) plants and maize protoplasts altered the expression of genes encoding enzymes involved in antioxidant catabolism, such as At1g02950, which encodes a glutathione transferase. Thus, the novel PTPase identified in this study is predicted to function in redox signaling in maize. [ABSTRACT FROM AUTHOR]

Published
2012

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