1. Anti-inflammatory Constituents from Caulis Trachelospermi.
- Author
-
Song H, Tan J, Ma R, Kennelly EJ, and Tan Q
- Subjects
- Animals, Inflammation drug therapy, Lipopolysaccharides, Macrophages metabolism, Mice, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, RAW 264.7 Cells, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Apocynaceae chemistry, Plant Extracts chemistry, Plant Extracts pharmacology
- Abstract
Caulis Trachelospermi, the stems with leaves of Trachelospermum jasminoides , is a well-known herbal drug of the Apocynaceae family recorded in the Chinese pharmacopeia and used for the treatment of inflammation-related diseases by ethnic minorities of China. The mechanism of anti-inflammatory activity and responsible constituents of T. jasminoides have not been well elucidated in previous studies. Preliminary investigation showed that both the water and the ethyl ester extracts of T. jasminoides exhibited potent inhibitory activity on nitric oxide (NO) production using lipopolysaccharide (LPS)-stimulated murine macrophages. Phytochemical investigation on these extracts afforded 23 compounds, including three new compounds (1: -3: ) identified on the basis of spectroscopic and mass spectrometric data. Anti-inflammatory bioassay showed that compounds 17, 18, 22: , and 23: inhibited significantly the production of NO in a concentration-dependent manner. Further studies indicated that compound 23: inhibited significantly TNF- α and IL-6 produced by LPS-stimulated RAW 264.7 cells with good selectivity, as well as protein expression of iNOS in RAW 264.7 cells. These chemical constituents may contribute to the anti-inflammatory potential of T. jasminoides ., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF