1. Gastroprotective Effect of Alkaloids from Cortex Phellodendri on Gastric Ulcers in Rats through Neurohumoral Regulation
- Author
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Fei Li, Chong-Zhi Wang, Chun-Feng Zhang, Xue Wang, Chun-Su Yuan, Ling Wang, Changrun Guo, Xin He, Wen-Juan Du, Jun Ji, Xiao-Ming Zhu, and Yan-Qi Liu
- Subjects
Male ,0301 basic medicine ,Pharmaceutical Science ,Pharmacology ,Analytical Chemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Oral administration ,Epidermal growth factor ,Drug Discovery ,heterocyclic compounds ,Medicinal plants ,Omeprazole ,Acetic Acid ,Neurotransmitter Agents ,biology ,Biochemistry ,Phellodendron amurense ,Molecular Medicine ,medicine.drug ,endocrine system ,complex mixtures ,03 medical and health sciences ,Acetic acid ,Alkaloids ,Phellodendron ,medicine ,Animals ,Stomach Ulcer ,Rutaceae ,Epidermal Growth Factor ,Plant Extracts ,business.industry ,organic chemicals ,Organic Chemistry ,Histology ,Anti-Ulcer Agents ,biology.organism_classification ,Rats ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Gastric Mucosa ,Carboxymethylcellulose Sodium ,Serotonin ,business ,030217 neurology & neurosurgery - Abstract
The present study aimed to investigate the gastroprotective activity of the total alkaloids from the bark of Phellodendron amurense and identify their possible mechanism. Total alkaloids were obtained through an alcohol extraction method and were analyzed using LC-MS/MS. Chronic gastric ulcers were induced by acetic acid (0.14 mol/L) filter paper on the gastric serosa. The antiulcer effect of total alkaloids was evaluated using the ulcer area, the ulcer inhibition ratio, and epidermal growth factor. The gastroprotective mechanism of total alkaloids was revealed using the levels of serotonin and noradrenaline. The results showed that oral administration of total alkaloids (30 mg/kg/day) obviously decreased the ulcer area (7.67 ± 2.06 mm 2 ; p 2 ). The ulcer inhibition ratio of the total alkaloids group (50 %) was higher than the omeprazole-treated group (46 %), which showed that the antiulcer effect of the total alkaloids may be superior to omeprazole. Besides, the total alkaloids significantly increased the epidermal growth factor level and accelerated the healing of ulcers. Histological examination of gastric tissues also supported the same conclusion. In addition, the total alkaloids significantly elevated the levels of serotonin and noradrenaline (both p
- Published
- 2016