1. Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry
- Author
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Min Ye, Xue Qiao, De-An Guo, Jing Dong, Yong-hong Liang, and Xionghao Lin
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Electrospray ionization ,Ethyl acetate ,Pharmaceutical Science ,Tandem mass spectrometry ,Mass spectrometry ,Lignans ,Analytical Chemistry ,chemistry.chemical_compound ,Phenols ,Polypodiaceae ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Drug Discovery ,Chemical composition ,Chromatography, High Pressure Liquid ,Flavonoids ,Pharmacology ,Chromatography, Reverse-Phase ,Chromatography ,Plant Extracts ,Organic Chemistry ,Triterpenes ,Complementary and alternative medicine ,Proanthocyanidin ,chemistry ,Molecular Medicine ,Rhizome - Abstract
The chemical composition of Drynaria fortunei, a traditional Chinese herbal medicine, is very complicated. In order to separate these chemicals to obtain their structural information, an orthogonal sample enrichment procedure was established. The ethyl acetate extract of D. fortunei was pre-separated by Sephadex LH-20 × polyamide columns to yield 15 fractions. These fractions were analyzed successively using a reversed-phase Agilent Zorbax SB-C18 column, coupled with diode array detection and electrospray ionization tandem mass spectrometry. The method reduced co-elution and enriched minor compounds on the basis of their chemical features. A total of 369 compounds were detected by LC/MSn, compared to less than 50 compounds without pre-separation. The pretreatment facilitated the analytical separation of flavonoids, proanthocyanidins, triterpenoids, phenolic acids, and lignans in D. fortunei, and allowed a comprehensive chemical profiling of these constituents. This method could be applied to other multicomponent herbal extracts.
- Published
- 2014