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Many common bean (Phaseolus vulgaris L.) plants cultivated in areas of the world with acidic soils exhibit difficulties adapting to low phosphorus (P) availability, along with aluminum (Al) toxicity, causing yield loss. The objective of this study was to evaluate the influence of an increase in P supply level on the agronomic, phenological, and physiological performance of two common bean breeding lines grown in acidic soil, with low fertility and under high temperature conditions, in a screenhouse. A randomized complete block (RCB) design was used under a factorial arrangement (five levels of P × 2 genotypes) for a total of 10 treatments with four replications. The factors considered in the experiment were: (i) five P supply levels (kg ha−1): four levels of P0, P15, P30, and P45 through the application of rock phosphate (RP), and one P level supplied through the application of organic matter (PSOM) corresponding to 25 kg P ha−1 (P25); and (ii) two advanced bean lines (BFS 10 and SEF10). Both bean lines were grown under the combined stress conditions of high temperatures (day and night maximum temperatures of 42.5 °C/31.1 °C, respectively) and acidic soil. By increasing the supply of P, a significant effect was found, indicating an increase in the growth and development of different vegetative organs, as well as physiological efficiency in photosynthesis and photosynthate remobilization, which resulted in higher grain yield in both bean lines evaluated (BFS 10 and SEF10). The adaptive responses of the two bean lines were found to be related to phenological adjustments (days to flowering and physiological maturity; stomatal development), as well as to heat dissipation strategies in the form of heat (NPQ) or unregulated energy (qN) that contributed to greater agronomic performance. We found that, to some extent, increased P supply alleviated the negative effects of high temperature on the growth and development of the reproductive organs of bean lines. Both bean lines (BFS 10 and SEF 10) showed adaptive attributes suited to the combined stress conditions of high temperature and acidic soil, and these two lines can serve as useful parents in a bean breeding program to develop multiple stress tolerant cultivars.

Increasing biodiversity in highly diverse plant communities can jointly increase ecosystem function and ecosystem vulnerability. This paradox requires further attention. This study analyzed the functional response of plant communities to above- and below-ground parameters along the chronosequence (degraded pastures (DP), early forests (EF), intermediate forests (IF), and old-growth forests (OF)) in two highly fragmented landscapes of the Colombian Amazon as an estimate of the level of functional vulnerability. Three sets of functional attributes were evaluated: (i) functional composition based on the community-weighted mean (CWM) of five traits; (ii) functional diversity based on the multi-trait indices and functional dispersion (FDis) of each individual trait; and (iii) the functional vulnerability at the community-level and species-level. The individual traits did not show a clear pattern along the chronosequence. However, the trend indicated an increase in the values of resource conservation traits with the age of abandonment. The functional response of the community did not vary between landscapes. Between DP and OF, there was a significant increase in functional diversity and a decrease in functional redundancy, which increased community-level vulnerability. Consequently, the more vulnerable species were observed in the IF and OF plots. In addition, a decrease in environmental parameters, such as penetration resistance, bulk density and Ca content, and an increase in slope, precipitation, electric conductivity, pH, clay, organic material, and P and N contents increased the vulnerability. We elucidated the need for secondary forest management in terms of conservation and restoration to maintain the capacity to respond to changing environmental conditions in highly fragmented landscapes in the Andean–Amazonian transition.

The evaluation of disease resistance is considered an important aspect of phenotyping for crop improvement. Identification of advanced lines of the common bean with disease resistance contributes to improved grain yields. This study aimed to determine the response of the photosynthetic apparatus to natural pathogen infection by using chlorophyll (Chla) fluorescence parameters and their relationship to the agronomic performance of 59 common bean lines and comparing the photosynthetic responses of naturally infected vs. healthy leaves. The study was conducted over two seasons under acid soil and high temperature conditions in the western Amazon region of Colombia. A disease susceptibility index (DSI) was developed and validated using chlorophyll a (Chla) fluorescence as a tool to identify Mesoamerican and Andean lines of common bean (Phaseolus vulgaris L.) that are resistant to pathogens. A negative effect on the functional status of the photosynthetic apparatus was found with the presence of pathogen infection, a situation that allowed the identification of four typologies based on the DSI values ((i) moderately resistant; (ii) moderately susceptible; (iii) susceptible; and (iv) highly susceptible). Moderately resistant lines, five of them from the Mesoamerican gene pool (ALB 350, SMC 200, BFS 10, SER 16, SMN 27) and one from the Andean gene pool (DAB 295), allocated a higher proportion of energy to photochemical processes, which increased the rate of electron transfer resulting in a lower sensitivity to disease stress. This photosynthetic response was associated with lower values of DSI, which translated into an increase in the accumulation of dry matter accumulation in different plant organs (leaves, stem, pods and roots). Thus, DSI values based on chlorophyll fluorescence response to pathogen infection could serve as a phenotyping tool for evaluating advanced common bean lines. Six common bean lines (ALB 350, BFS 10, DAB 295, SER 16, SMC 200 and SMN 27) were identified as less sensitive to disease stress under field conditions in the western Amazon region of Colombia, and these could serve as useful parents for improving the common bean for multiple stress resistance.

Common bean (Phaseolus vulgaris L.) is sensitive to different types of abiotic stresses (drought, high temperature, low soil fertility, and acid soil), and this may limit its adaptation and consequently to its yield under stress. Because of this, a sister species, tepary bean (Phaseolus acutifolius A. Gray), has recently gained attention in breeding for improved abiotic stress tolerance in common bean. In this study, we evaluated the adaptation of 302 accessions of tepary bean (Phaseolus acutifolius A. Gray) and its wild relatives (grouped in four types of tepary bean genetic resource: cultivated, acutifolius regressive, acutifolius wild, tenuifolius wild) when grown under high temperature and acid soil conditions with aluminum toxicity in the Amazon region of Colombia. Our objective was to determine differences among four types of tepary bean genetic resource in their morpho-phenological, agronomic, and physiological responses to combined high temperature and acid soil stress conditions. We found that cultivated P. acutifolius var acutifolius presented a greater number of pods per plant, as well as larger seeds and a greater number of seeds per pod. Some traits, such as root biomass, days to flowering and physiological maturity, specific leaf area, and stomatal density, showed significant differences between types of tepary bean genetic resource, probably contributing to difference in adaptation to combined stress conditions of high temperature and acid soil conditions. The photochemical quenching (qP) was higher in cultivated P. acutifolius var. acutifolius, while energy dissipation by non-photochemical quenching (NPQ) in the form of heat and the coefficient of non-photochemical dissipation (qN) were higher in acutifolius regressive and tenuifolius wild accessions. We have identified 6 accessions of cultivated and 19 accessions of tenuifolius wild that exhibited grain yields above 1800 kg ha−1. These accessions could be suitable to use as parents to improve dry seed production of tepary bean under combined stress conditions of high temperature and acid soil.

Knowledge of the physiological basis for improved genetic adaptation of common bean (Phaseolus vulgaris L.) lines to acid soils and high temperature conditions in the Amazon region of Colombia is limited. In this study, we evaluated the differences among 41 common bean lines in energy use, leaf cooling, photosynthate partitioning to pod formation and grain filling, and grain yield over two seasons under acid soil and high temperature stress in the Amazon region of Colombia. Common bean lines evaluated included medium and large seeded interspecific lines of Mesoamerican and Andean gene pools with different levels of adaptation to abiotic stress conditions and some lines are improved for iron and zinc (biofortified) concentration in seeds. We found three bean lines (GGR 147, SMG 21 and SMG 12) that were superior in their photosynthetic response, leaf cooling, photosynthate partitioning ability to pod formation and grain filling, resulting in grain yields exceeding 1900 kg ha−1 under acid soil and high temperature stress conditions. The superior photosynthetic performance was attributed to the efficient use of absorbed energy on the electron level in thylakoids, which is mainly oriented to a higher quantum yield of PSII (ΦII), lower energy dissipation in the form of heat (ΦNPQ), high linear electron flow (LEF) and high fraction of PSI centers in open state (PSIopen). We speculate that these photosynthetic and photosynthate partitioning responses of superior bean lines are part of the genetic adaptation to acidic soils and high temperature stress conditions. Among the evaluated bean lines, three lines (GGR 147, SMG 21 and SMG 12) combined the desirable attributes for genetic improvement of stress tolerance and biofortification. These lines can serve as parents to further improve traits (energy use efficiency and multiple stress resistance) that are important for bean production in the Amazon region.