1. Complete sequence of pEC14_114, a highly conserved IncFIB/FIIA plasmid associated with uropathogenic Escherichia coli cystitis strains.
- Author
-
DebRoy, Chitrita, Sidhu, Mandeep S., Sarker, Upal, Jayarao, Bhushan M., Stell, Adam L., Bell, Nathan P., and Johnson, Timothy J.
- Subjects
- *
PLASMID genetics , *NUCLEOTIDE sequence , *PATHOGENIC bacteria , *ESCHERICHIA coli , *CYSTITIS , *MICROBIAL sensitivity tests , *MOBILE genetic elements , *MICROBIAL virulence - Abstract
Abstract: Extraintestinal pathogenic Escherichia coli (ExPEC) are known to cause important diseases of humans and animals, and they have been shown to carry a variety of plasmids associated with increased virulence and decreased antimicrobial susceptibility. Here, the completed DNA sequence of a human uropathogenic E. coli (UPEC; O6:H31 isolate) plasmid, pEC14_114, was determined. The plasmid was 114,222bp in length and was highly similar to plasmid sequences or draft contiguous sequences from three other human cystitis-associated UPEC isolates. pEC14_114 contained 141 coding regions, including a number of genes associated with mobile genetic elements, F-type transfer, plasmid maintenance and stability, colicin immunity, and plasmid replication. This plasmid also possessed a “genetic load” region containing genes with predicted similarity to iron acquisition systems and virulence factors. The prevalence of pEC14-associated genes was determined for a collection of 1456 E. coli isolates, including those from food products, humans, dogs, cats, pigs, chickens, and turkeys. pEC14_114-associated genes were found significantly more often (16–35%) among human UPEC and neonatal meningitis-associated isolates than among food- and animal-source isolates (0–8%). Overall, this plasmid represents a novel IncFIB/FIIA plasmid type associated with human ExPEC belonging to the B2 phylogenetic group. The overall role of this plasmid, if any, in human ExPEC infections remains to be determined. [Copyright &y& Elsevier]
- Published
- 2010
- Full Text
- View/download PDF