1. Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses.
- Author
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Luo, Hao, Hansen, Anne Sofie L., Yang, Lei, Schneider, Konstantin, Kristensen, Mette, Christensen, Ulla, Christensen, Hanne B., Du, Bin, Özdemir, Emre, Feist, Adam M., Keasling, Jay D., Jensen, Michael K., Herrgård, Markus J., and Palsson, Bernhard O.
- Subjects
ADENOSYLMETHIONINE ,METHYLATION ,ENZYMES ,ESCHERICHIA coli ,METHYLTRANSFERASES - Abstract
We present a selection design that couples S-adenosylmethionine–dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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