4 results
Search Results
2. BIM Mediates EGFR Tyrosine Kinase Inhibitor-Induced Apoptosis in Lung Cancers with Oncogenic EGFR Mutations.
- Author
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Costa, Daniel B., Halmos, Balázs, Kumar, Amit, Schumer, Susan T., Huberman, Mark S., Boggon, Titus J., Tenen, Daniel G., and Kobayashi, Susumu
- Subjects
POLYPEPTIDES ,PROTEIN-tyrosine kinases ,CELL death ,EPIDERMAL growth factor ,TUMORS - Abstract
Susumu Kobayashi and colleagues provide evidence that the polypeptide BIM is involved in tyrosine kinase inhibitor (TKI)-induced apoptosis in sensitiveEGFR-mutant cells and suggest that induction of BIM may have a role in the treatment of TKI-resistant tumors. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
3. Interleukin-1 Stimulates ß-Cell Necrosis and Release of the Immunological Adjuvant HMGB1.
- Author
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Steer, Sarah A., Scarim, Anna L., Chambers, Kari T., and Corbett, John A.
- Subjects
INTERLEUKIN-1 ,CELL death ,PANCREATIC beta cells ,NITRIC oxide ,NECROSIS - Abstract
Background There are at least two phases of β-cell death during the development of autoimmune diabetes: an initiation event that results in the release of β-cell-specific antigens, and a second, antigen-driven event in which β-cell death is mediated by the actions of T lymphocytes. In this report, the mechanisms by which the macrophage-derived cytokine interleukin (IL)-1 induces β-cell death are examined. IL-1, known to inhibit glucose-induced insulin secretion by stimulating inducible nitric oxide synthase expression and increased production of nitric oxide by β-cells, also induces β-cell death. Methods and Findings To ascertain the mechanisms of cell death, the effects of IL-1 and known activators of apoptosis on β-cell viability were examined. While IL-1 stimulates β-cell DNA damage, this cytokine fails to activate caspase-3 or to induce phosphatidylserine (PS) externalization; however, apoptosis inducers activate caspase-3 and the externalization of PS on β-cells. In contrast, IL-1 stimulates the release of the immunological adjuvant high mobility group box 1 protein (HMGB1; a biochemical maker of necrosis) in a nitric oxide-dependent manner, while apoptosis inducers fail to stimulate HMGB1 release. The release of HMGB1 by β-cells treated with IL-1 is not sensitive to caspase-3 inhibition, while inhibition of this caspase attenuates βcell death in response to known inducers of apoptosis. Conclusions These findings indicate that IL-1 induces β-cell necrosis and support the hypothesis that macrophage-derived cytokines may participate in the initial stages of diabetes development by inducing β-cell death by a mechanism that promotes antigen release (necrosis) and islet inflammation (HMGB1 release). [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
4. Macrophages inhibit neovascularization in a murine model of age-related macular degeneration.
- Author
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Apte, Rajendra S., Richter, Jennifer, Herndon, John, and Ferguson, Thomas A.
- Subjects
RETINAL degeneration ,BLINDNESS ,NEOVASCULARIZATION ,MACROPHAGES ,LIGANDS (Biochemistry) ,BLOOD vessels ,TUMOR growth ,INTERLEUKIN-10 ,PROTEIN metabolism ,AGING ,ANIMAL experimentation ,BIOLOGICAL models ,CELL death ,COMPARATIVE studies ,DOCUMENTATION ,INJECTIONS ,INTERLEUKINS ,RESEARCH methodology ,MEDICAL cooperation ,MICE ,RESEARCH ,RESEARCH funding ,RETINA ,EVALUATION research ,PATHOLOGIC neovascularization - Abstract
Background: Age-related macular degeneration (AMD) is the leading cause of blindness in people over 50 y of age in at least three continents. Choroidal neovascularization (CNV) is the process by which abnormal blood vessels develop underneath the retina. CNV develops in 10% of patients with AMD but accounts for up to 90% of the blindness from AMD. Although the precise etiology of CNV in AMD remains unknown, the macrophage component of the inflammatory response, which has been shown to promote tumor growth and support atherosclerotic plaque formation, is thought to stimulate aberrant angiogenesis in blinding eye diseases. The current theory is that macrophage infiltration promotes the development of neovascularization in CNV.Methods and Findings: We examined the role of macrophages in a mouse model of CNV. IL-10(-/-) mice, which have increased inflammation in response to diverse stimuli, have significantly reduced CNV with increased macrophage infiltrates compared to wild type. Prevention of macrophage entry into the eye promoted neovascularization while direct injection of macrophages significantly inhibited CNV. Inhibition by macrophages was mediated by the TNF family death molecule Fas ligand (CD95-ligand).Conclusions: Immune vascular interactions can be highly complex. Normal macrophage function is critical in controlling pathologic neovascularization in the eye. IL-10 regulates macrophage activity in the eye and is an attractive therapeutic target in order to suppress or inhibit CNV in AMD that can otherwise lead to blindness. [ABSTRACT FROM AUTHOR]- Published
- 2006
- Full Text
- View/download PDF
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