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Background Crimean-Congo Haemorrhagic fever Virus (CCHFV) is a rapidly emerging vector-borne pathogen and the cause of a virulent haemorrhagic fever affecting large parts of Europe, Africa, the Middle East and Asia. Methodology/principle findings An isothermal recombinase polymerase amplification (RPA) assay was successfully developed for molecular detection of CCHFV. The assay showed rapid (under 10 minutes) detection of viral extracts/synthetic virus RNA of all 7 S-segment clades of CCHFV, with high target specificity. The assay was shown to tolerate the presence of inhibitors in crude preparations of mock field samples, indicating that this assay may be suitable for use in the field with minimal sample preparation. The CCHFV RPA was successfully used to screen and detect CCHFV positives from a panel of clinical samples from Tajikistan. Conclusions/significance The assay is a rapid, isothermal, simple-to-perform molecular diagnostic, which can be performed on a light, portable real-time detection device. It is ideally placed therefore for use as a field-diagnostic or in-low resource laboratories, for monitoring of CCHF outbreaks at the point-of-need, such as in remote rural regions in affected countries., Author summary The Crimean-Congo Haemorrhagic fever (CCHF) recombinase polymerase amplification assay is a new, rapid and portable diagnostic method. It has been developed for the detection of infection with CCHF virus (CCHFV), the cause of a deadly haemorrhagic disease in humans and an emerging global health threat. As a rapid diagnostic suitable for use on a portable and lightweight detection device, this has the potential to be used for fast-turnaround diagnosis at the point of need, providing timely results to clinicians at the bedside and preventing the spread of virus in the hospital setting. As a portable diagnostic it would also enable the diagnosis of CCHFV to be taken out of the laboratory during an outbreak, enabling testing in field laboratories or community clinics.