1. A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.
- Author
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Dang My Nhi, Nguyen Tien Huy, Kaname Ohyama, Daisuke Kimura, Nguyen Thi Phuong Lan, Leo Uchida, Nguyen Van Thuong, Cao Thi My Nhon, Le Hong Phuc, Nguyen Thi Mai, Shusaku Mizukami, Lam Quoc Bao, Nguyen Ngoc Doan, Nguyen Van Thanh Binh, Luong Chan Quang, Juntra Karbwang, Katsuyuki Yui, Kouichi Morita, Vu Thi Que Huong, and Kenji Hirayama
- Subjects
Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
BACKGROUND:Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY:Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6-48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group. Validation was undertaken in 6 SD-SPL and 10 DWS patients. PRINCIPAL FINDINGS:Nineteen plasma proteins exhibited significantly different relative concentrations (p
- Published
- 2016
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