1. Alleviation of Rosup-induced oxidative stress in porcine granulosa cells by anthocyanins from red-fleshed apples
- Author
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Wei Shen, Ya Xiang, Yapeng Li, Heqiang Huo, Hongyi Dai, Lei-Lei Yang, Gui-Fang He, Zhu Jun, Yugang Zhang, and Fang-Nong Lai
- Subjects
0106 biological sciences ,0301 basic medicine ,Chemical Radicals ,GPX1 ,Swine ,lcsh:Medicine ,Apoptosis ,medicine.disease_cause ,Biochemistry ,Physical Chemistry ,01 natural sciences ,Antioxidants ,Anthocyanins ,Oxidative Damage ,chemistry.chemical_compound ,Fluorescence Microscopy ,lcsh:Science ,chemistry.chemical_classification ,Microscopy ,Multidisciplinary ,Cell Death ,biology ,Chemistry ,Glutathione peroxidase ,Light Microscopy ,food and beverages ,Plants ,Catalase ,Glutathione ,Enzymes ,Dismutases ,Cell Processes ,Malus ,Physical Sciences ,Female ,Research Article ,Free Radicals ,SOD1 ,Research and Analysis Methods ,Fruits ,Superoxide dismutase ,03 medical and health sciences ,medicine ,Animals ,Glutathione Peroxidase ,Reactive oxygen species ,Granulosa Cells ,Apples ,Plant Extracts ,Superoxide Dismutase ,fungi ,lcsh:R ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Oxidative Stress ,030104 developmental biology ,Enzymology ,biology.protein ,lcsh:Q ,Reactive Oxygen Species ,Oxidative stress ,010606 plant biology & botany - Abstract
Anthocyanins are the polyphenolic phytochemicals which have been shown to scavenge free radicals. In this study, we investigated the effects of anthocyanins extracted from red-fleshed apples (Malus sieversii) on reducing oxidative damage by Rosup in porcine granulosa cells (GCs) by measuring intracellular reactive oxygen species (ROS), content of glutathione (GSH), activities of superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPX1) and the gene expression of SOD1, CAT, GPX1. Apoptosis was determined with TdT-mediated dUTP-biotin nick end labeling (TUNEL) and apoptosis-related proteins were quantified with Western blotting. The results indicate that Rosup increases oxidative stress by inducing reactive oxygen species production in porcine GCs and the oxidative stress could be reduced by anthocyanins. The gene expression of SOD1, CAT, GPX1 and the activities of these enzymes were increased when GCs were treated with anthocyanins and Rosup for 6 hours. Anthocyanins inhibit Rosup-induced apoptosis by increasing expression of antiapoptotic protein Bcl-2 and suppressing the expression of pro-apoptotic protein Bax. Collectively, anthocyanins from red-fleshed apples reduce oxidative stress and inhibit apoptosis in porcine GCs in vitro. This approach indicates that antioxidants might be developed from red-fleshed apples.
- Published
- 2017