49 results on '"WEI GAO"'
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2. Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu.
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Ruipei Yang, Jie Wang, Wei Gao, Ye Jiang, Jing Su, Dongmei Sun, and Guozhen He
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Medicine ,Science - Abstract
ObjectiveTo explore the influence of biological characteristics on the yield of Amomum villosum Lour. and Amomum longiligulare T. L. Wu, to find an effective pollen viability evaluation method and storage method to solve the problem of the low yield of Amomum plants.MethodsFive germplasm of Amomum plants were used to investigate the effects of the phenological phase, pollen viability, and stigma receptivity on natural and artificial fruit set.ResultsAmomum longiligulare T. L. Wu showed late flowering, and its natural pollination rate is higher than that of Amomum villosum Lour. In all germplasm, the artificial pollination rate and fruit setting rate are more than 3 times higher than that under natural conditions. Fruits begin to drop seven days after successful pollination, and the fruit drop is basically stable after one month. The hybridization verification showed that TTC method was simpler and more accurate than in vitro germination method. Optimal storage conditions for pollen are 4°C and high humidity. After 36 h of storage, pollen can still be used for artificial cross-pollination or as hybrid parents.ConclusionThe special biological characteristics are the fundamental reason for the low natural pollination rate of Amomum plants. The accurate measurement method of Amomum plants pollen is the TTC method, and storage at 4°C and high humidity can increase the yield, which was six times that of the natural yield.
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- 2021
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3. Substitution of manure for chemical fertilizer affects soil microbial community diversity, structure and function in greenhouse vegetable production systems.
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Haoan Luan, Wei Gao, Shaowen Huang, Jiwei Tang, Mingyue Li, Huaizhi Zhang, Xinping Chen, and Dainius Masiliūnas
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Medicine ,Science - Abstract
Soil microbial communities and enzyme activities together affect various ecosystem functions of soils. Fertilization, an important agricultural management practice, is known to modify soil microbial characteristics; however, inconsistent results have been reported. The aim of this research was to make a comparative study of the effects of different nitrogen (N) fertilizer rates and types (organic and inorganic) on soil physicochemical properties, enzyme activities and microbial attributes in a greenhouse vegetable production (GVP) system of Tianjin, China. Results showed that manure substitution of chemical fertilizer, especially at a higher substitution rate, improved soil physicochemical properties (higher soil organic C (SOC) and nutrient (available N and P) contents; lower bulk densities), promoted microbial growth (higher total phospholipid fatty acids and microbial biomass C contents) and activity (higher soil hydrolase activities). Manure application induced a higher fungi/bacteria ratio due to a lower response in bacterial than fungal growth. Also, manure application greatly increased bacterial stress indices, as well as microbial communities and functional diversity. The principal component analysis showed that the impact of manure on microbial communities and enzyme activities were more significant than those of chemical fertilizer. Furthermore, redundancy analysis indicated that SOC and total N strongly influenced the microbial composition, while SOC and ammonium-N strongly influenced the microbial activity. In conclusion, manure substitution of inorganic fertilizer, especially at a higher substitution rate, was more efficient for improving soil quality and biological functions.
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- 2020
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4. The improvement of the shear stress and oscillatory shear index of coronary arteries during Enhanced External Counterpulsation in patients with coronary heart disease.
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Ling Xu, Xi Chen, Ming Cui, Chuan Ren, Haiyi Yu, Wei Gao, Dongguo Li, and Wei Zhao
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Medicine ,Science - Abstract
BACKGROUND:Enhanced External Counterpulsation (EECP) can chronically relieve ischemic chest pain and improve the prognosis of coronary heart disease (CHD). Despite its role in mitigating heart complications, EECP and the mechanisms behind its therapeutic nature, such as its effects on blood flow hemodynamics, are still not fully understood. This study aims to elucidate the effect of EECP on significant hemodynamic parameters in the coronary arterial tree. METHODS:A finite volume method was used in conjunction with the inlet pressure wave (surrogated by the measured aortic pressure) before and during EECP and outlet flow resistance, assuming the blood as newtonian fluid. The time-average wall shear stress (TAWSS) and oscillatory shear index (OSI) were determined from the flow field. RESULTS:Regardless of the degree of vascular stenosis, hemodynamic conditions and flow patterns could be improved during EECP. In comparison with the original tree, the tree with a severe stenosis (75% area stenosis) demonstrated more significant improvement in hemodynamic conditions and flow patterns during EECP, with surface area ratio of TAWSS risk area (SAR-TAWSS) reduced from 12.3% to 6.7% (vs. SAR-TAWSS reduced from 7.2% to 5.6% in the original tree) and surface area ratio of OSI risk area (SAR-OSI) reduced from 6.8% to 2.5% (vs. SAR-OSI of both 0% before and during EECP in the original tree because of mild stenosis). Moreover, it was also shown that small ratio of diastolic pressure (D) and systolic pressure (S) (D/S) could only improve the hemodynamic condition mildly. The SAR-TAWSS reduction ratio significantly increased as D/S became larger. CONCLUSIONS:A key finding of the study was that the improvement of hemodynamic conditions along the LMCA trees during EECP became more significant with the increase of D/S and the severity degree of stenoses at the bifurcation site. These findings have important implications on EECP as adjuvant therapy before or after percutaneous coronary intervention (PCI) in patients with diffuse atherosclerosis.
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- 2020
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5. Implementation of a complex intervention to improve care for patients whose situations are clinically uncertain in hospital settings: A multi-method study using normalisation process theory.
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Halle Johnson, Emel Yorganci, Catherine J Evans, Stephen Barclay, Fliss E M Murtagh, Deokhee Yi, Wei Gao, Elizabeth L Sampson, Joanne Droney, Morag Farquhar, and Jonathan Koffman
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Medicine ,Science - Abstract
PurposeTo examine the use of Normalisation Process Theory (NPT) to establish if, and in what ways, the AMBER care bundle can be successfully normalised into acute hospital practice, and to identify necessary modifications to optimise its implementation.MethodMulti-method process evaluation embedded within a mixed-method feasibility cluster randomised controlled trial in two district general hospitals in England. Data were collected using (i) focus groups with health professionals (HPs), (ii) semi-structured interviews with patients and/or carers, (iii) non-participant observations of multi-disciplinary team meetings and (iv) patient clinical note review. Thematic analysis and descriptive statistics, with interpretation guided by NPT components (coherence; cognitive participation; collective action; reflexive monitoring). Data triangulated across sources.ResultsTwo focus groups (26 HPs), nine non-participant observations, 12 interviews (two patients, 10 relatives), 29 clinical note reviews were conducted. While coherence was evident, with HPs recognising the value of the AMBER care bundle, cognitive participation and collective action presented challenges. Specifically: (1) HPs were unable and unwilling to operationalise the concept of 'risk of dying' intervention eligibility criteria (2) integration relied on a 'champion' to drive participation and ensure sustainability; and (3) differing skills and confidence led to variable engagement with difficult conversations with patients and families about, for example, nearness to end of life. Opportunities for reflexive monitoring were not routinely embedded within the intervention. Reflections on the use of the AMBER care bundle from HPs and patients and families, including recommended modifications became evident through this NPT-driven analysis.ConclusionTo be successfully normalised, new clinical practices, such as the AMBER care bundle, must be studied within the wider context in which they operate. NPT can be used to the aid identification of practical strategies to assist in normalisation of complex interventions where the focus of care is on clinical uncertainty in acute hospital settings.
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- 2020
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6. Drought stress has transgenerational effects on soybean seed germination and seedling vigor.
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Chathurika Wijewardana, K Raja Reddy, L Jason Krutz, Wei Gao, and Nacer Bellaloui
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Medicine ,Science - Abstract
Effects of environmental stressors on the parent may be transmitted to the F1 generation of plants that support global food, oil, and energy production for humans and animals. This study was conducted to determine if the effects of drought stress on parental soybean plants are transmitted to the F1 generation. The germination and seedling vigor of F1 soybean whose maternal parents, Asgrow AG5332 and Progeny P5333RY, were exposed to soil moisture stress, that is, 100, 80, 60, 40, and 20% replacement of evapotranspiration (ET) during reproductive growth, were evaluated under controlled conditions. Pooled over cultivars, effects of soil moisture stress on the parents caused a reduction in the seed germination rate, maximum seed germination, and overall seedling performance in the F1 generation. The effect of soil moisture stress on the parent environment induced seed quality that carried on the F1 generation seed gemination and seedling traits under optimum conditions and further exasperated when exposed to increasing levels of drought stress. Results indicate that seed weight and storage reserve are key factors positively associated with germination traits and seedling growth. Our data confirm that the effects of soil moisture stress on soybean are transferable, causing reduced germination, seedling vigor, and seed quality in the F1 generation. Therefore, optimal water supply during soybean seed formation period may be beneficial for seed producers in terms of optimizing seed quality and vigor characteristics of commodity seed.
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- 2019
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7. Low-rank graph optimization for multi-view dimensionality reduction.
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Youcheng Qian, Xueyan Yin, Jun Kong, Jianzhong Wang, and Wei Gao
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Medicine ,Science - Abstract
Graph-based dimensionality reduction methods have attracted substantial attention due to their successful applications in many tasks, including classification and clustering. However, most classical graph-based dimensionality reduction approaches are only applied to data from one view. Hence, combining information from different data views has attracted considerable attention in the literature. Although various multi-view graph-based dimensionality reduction algorithms have been proposed, the graph construction strategies utilized in them do not adequately take noise and different importance of multiple views into account, which may degrade their performance. In this paper, we propose a novel algorithm, namely, Low-Rank Graph Optimization for Multi-View Dimensionality Reduction (LRGO-MVDR), that overcomes these limitations. First, we construct a low-rank shared matrix and a sparse error matrix from the graph that corresponds to each view for capturing potential noise. Second, an adaptive nonnegative weight vector is learned to explore complementarity among views. Moreover, an effective optimization procedure based on the Alternating Direction Method of Multipliers scheme is utilized. Extensive experiments are carried out to evaluate the effectiveness of the proposed algorithm. The experimental results demonstrate that the proposed LRGO-MVDR algorithm outperforms related methods.
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- 2019
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8. Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells.
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Ze-Wen Yu, Wei Gao, Xin-Yao Feng, Jin-Yu Zhang, Hai-Xiang Guo, Chang-Jiang Wang, Jian Chen, Jin-Ping Hu, Wen-Zhi Ren, and Bao Yuan
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Medicine ,Science - Abstract
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
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- 2019
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9. Myeloperoxidase mediated HDL oxidation and HDL proteome changes do not contribute to dysfunctional HDL in Chinese subjects with coronary artery disease.
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Guisong Wang, Anna Vachaparampil Mathew, Haiyi Yu, Lei Li, Liyun He, Wei Gao, Xiaodan Liu, Yanhong Guo, Jaeman Byun, Jifeng Zhang, Y Eugene Chen, and Subramaniam Pennathur
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Medicine ,Science - Abstract
High density lipoprotein (HDL) cholesterol levels and cholesterol efflux capacity (CEC) are inversely correlated with coronary artery disease (CAD) risk. Myeloperoxidase (MPO) derived oxidants and HDL proteome changes are implicated in HDL dysfunction in subjects with CAD in the United States; however, the effect of MPO on HDL function and HDL proteome in ethnic Chinese population is unknown. We recruited four matched ethnic Chinese groups (20 patients each): subjects with 1) low HDL levels (HDL levels in men 50mg/dL; women >60mg/dL) with no CAD; and 4) high HDL with CAD. Serum cytokines, serum MPO levels, serum CEC, MPO-oxidized HDL tyrosine moieties, and HDL proteome were assessed by mass spectrometry individually in the four groups. The cytokines, MPO levels, and HDL proteome profiles were not significantly different between the four groups. As expected, CEC was depressed in the entire CAD group but more specifically in the CAD low-HDL group. HDL of CAD subjects had significantly higher 3-nitrotyrosine than non-CAD subjects, but the MPO-specific 3-chlorotyrosine was unchanged; CEC in the CAD low-HDL group did not correlate with either HDL 3-chlorotyrosine or 3-nitrotyrosine levels. Neither 3-chlorotyrosine, which is MPO-specific, nor 3-nitrotyrosine generated from MPO or other reactive nitrogen species was associated with CEC. MPO mediated oxidative stress and HDL proteome composition changes are not the primary cause HDL dysfunction in Chinese subjects with CAD. These studies highlight ethnic differences in HDL dysfunction between United States and Chinese cohorts raising possibility of unique pathways of HDL dysfunction in this cohort.
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- 2018
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10. A two-stage classification method for borehole-wall images with support vector machine.
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Zhaopeng Deng, Maoyong Cao, Laxmisha Rai, and Wei Gao
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Medicine ,Science - Abstract
Analyzing geological drilling hole images acquired by Axial View Panoramic Borehole Televiewer (APBT) is a key step to explore the geological structure in a geological exploration. Conventionally, the borehole images are examined by technicians, which is inefficient and subjective. In this paper, three dominant types of borehole-wall images on coal-rock mass structure, namely, border images, fracture images and intact rock mass images are mainly studied. The traditional image classification methods based on unified feature extraction algorithm and single classifier is not effect for the borehole images. Therefore, this paper proposes a novel two-stage classification approach to improve the classification performance of borehole images. In the first-stage classification, the border images are identified from three kinds of images based on texture features and gray-scale histograms features. For the remaining two types of images, in the second-stage classification, Gabor filter is first applied to segment the region of interest (ROI) (such as microfracture, absciss layer and horizontal cracks, etc.) and the central interference region. Then, using the same feature vector after eliminating the central interference region, fracture images are separated from intact rock mass images. We test our two-stage classification system with real borehole images. The results of experimental show that the two-stage classification method can effectively classify three major borehole-wall images with the correction rate of 95.55% in the first stage and 95% in the second stage.
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- 2018
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11. Cutaneous transcriptome analysis in NIH hairless mice.
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Zhong-Hao Ji, Jian Chen, Wei Gao, Jin-Yu Zhang, Fu-Shi Quan, Jin-Ping Hu, Bao Yuan, and Wen-Zhi Ren
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Medicine ,Science - Abstract
Mice with spontaneous coat mutations are ideal animal models for studying skin development and tumorigenesis. In this study, skin hair growth cycle abnormalities were examined in NIH hairless mice 42 days after birth (P42) by using hematoxylin-eosin (H&E) staining. To examine the gene expression patterns in the skin of mutant mice, the dorsal skin of P42 female NIH mice and NIH hairless mice was sequenced by RNA-Seq, and 5,068 differentially expressed genes (DEGs) were identified (false discovery rate [FDR] ≥ 2, P < 0.05). A pathway analysis showed that basal cell carcinoma, the cell cycle and the Hippo, Hedgehog and Wnt signaling pathways were up-regulated in NIH hairless mice. Previous studies have shown that these pathways are closely associated with cell proliferation, cell cycle, organ size and cancer development. In contrast, signal transduction, bacterial and parasitic infection, and receptor-mediated pathways, including calcium signaling, were down-regulated in NIH hairless mice. A gene interaction network analysis was performed to identify genes related to hair follicle development. To verify the reliability of the RNA-Seq results, we used q-PCR to analyze 12 key genes identified from the gene interaction network analysis, including eight down-regulated and four up-regulated genes, and the results confirmed the reliability of the RNA-Seq results. Finally, we constructed the differential gene expression profiles of mutant mice by RNA-Seq. NIH hairless mice exhibited abnormalities in hair development and immune-related pathways. Pik3r1 and Pik3r3 were identified as key genes, laying the foundation for additional in-depth studies of hairless mice.
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- 2017
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12. Downregulation of microRNA-145 may contribute to liver fibrosis in biliary atresia by targeting ADD3.
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Yongqin Ye, Zhihan Li, Qi Feng, Zimin Chen, Zhouguang Wu, Jianyao Wang, Xiaoshuo Ye, Dahao Zhang, Lei Liu, Wei Gao, Lihui Zhang, and Bin Wang
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Medicine ,Science - Abstract
Biliary atresia (BA) is a pediatric liver disease characterized by fibro-obliteration and obstruction of the extrahepatic biliary system, that invariably leads to cirrhosis and even death, if left untreated for extended time. However, its pathology and etiology still remained unknown. In this study, we tested the expression of adducin 3 (ADD3), the gene identified as a susceptibility gene in BA by GWAS, and uncovered its upstream regulatory microRNA in the pathogenesis of BA.In this study, 14 infants with BA and 14 infants with choledochal cyst (CC) were enrolled as experimental group and control group, respectively. ADD3 and microRNA-145 (miR-145) expression profiles in liver tissues of BA and CC were determined using qPCR. Luciferase reporter assay was performed to verify the direct interaction between miR-145-5p and ADD3 3' Untranslated Regions (3'UTR). The Lentiviral vectors containing miR-145, miR-145-3p inhibitor, miR-145-5p inhibitor, empty vector were transfected into human hepatic stellate cell line (LX-2) to determine the functional effect of miR-145 on ADD3 expression at both mRNA and protein level.MiR-145 was shown to be down-regulated in liver tissues of infants with BA compared to CC (p = 0.0267). ADD3, verified as a target of miR-145-5p, was shown to be overexpressed in infants with BA at the mRNA level (p = 0.0118). Transfection of lentiviruses containing miR-145 into LX-2 cells decreased the expression of ADD3 at both mRNA and protein level compared to negative control group, and suppressed the expression of p-Akt at protein level.Our study has shown that overexpressed ADD3 and downregulated miR-145 were detected in BA liver tissues. MiR-145-5p was confirmed to target ADD3 by luciferase reporter assay. The downregulation of miR-145 may contribute to liver fibrosis in BA by upregulating the expression of ADD3.
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- 2017
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13. Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa).
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Lijun Zou, Xiaocheng Wang, Liping Jiang, Shengping Wang, Xia Xiong, Huansheng Yang, Wei Gao, Min Gong, Chien-An A Hu, and Yulong Yin
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Medicine ,Science - Abstract
Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of tumors. In this study, the full-length cDNA of Sus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that the Sfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain. Tissue distribution analysis showed that Sfz6 mRNA is ubiquitously expressed in various tissues, being highest in kidney, moderate in jejunum, ileum, colon, liver, and spleen. However, FZD6 protein is highly expressed in the heart and there was no significant difference in other tissues. The relative abundance and localization of FZD6 protein in jejunum along the crypt-villus axis was determined by Western blot and immunohistochemical localization. The results show that in the jejunum, FZD6 protein is highly expressed in the villus and less in the crypt cells. Cellular proliferation and viability assays indicate that knockdown of FZD6 with small interfering RNAs (siRNA) significantly reduced the cell viability of the intestinal porcine enterocyte cells (IPEC-J2). Furthermore, qPCR and Western blot analysis revealed that expressions of ras-related C3 botulinum toxin substrate 1 (Rac1); ras homolog gene family member A (RhoA) and c-Jun N-terminal kinase 1 (JNK1), some components of PCP signaling pathway were upregulated (P < 0.05) by knockdown of FZD6 in IPEC-J2 cells. In conclusion, these results showed that FZD6 abundance in the villus was higher than that in crypt cells and knockdown of FZD6 induces PCP signal pathway components expression in IPEC-J2 cells. Our findings provide the foundation for further investigation into porcine FZD6 gene.
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- 2017
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14. Poly(ADP-Ribose) Glycohydrolase (PARG) Silencing Suppresses Benzo(a)pyrene Induced Cell Transformation.
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Xuan Li, Xiyi Li, Zhiliang Zhu, Peiwu Huang, Zhixiong Zhuang, Jianjun Liu, Wei Gao, Yinpin Liu, and Haiyan Huang
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Medicine ,Science - Abstract
Benzo(a)pyrene (BaP) is a ubiquitously distributed environmental pollutant and known carcinogen, which can induce malignant transformation in rodent and human cells. Poly(ADP-ribose) glycohydrolase (PARG), the primary enzyme that catalyzes the degradation of poly(ADP-ribose) (PAR), has been known to play an important role in regulating DNA damage repair and maintaining genomic stability. Although PARG has been shown to be a downstream effector of BaP, the role of PARG in BaP induced carcinogenesis remains unclear. In this study, we used the PARG-deficient human bronchial epithelial cell line (shPARG) as a model to examine how PARG contributed to the carcinogenesis induced by chronic BaP exposure under various concentrations (0, 10, 20 and 40 μM). Our results showed that PARG silencing dramatically reduced DNA damages, chromosome abnormalities, and micronuclei formations in the PARG-deficient human bronchial epithelial cells compared to the control cells (16HBE cells). Meanwhile, the wound healing assay showed that PARG silencing significantly inhibited BaP-induced cell migration. Furthermore, silencing of PARG significantly reduced the volume and weight of tumors in Balb/c nude mice injected with BaP induced transformed human bronchial epithelial cells. This was the first study that reported evidences to support an oncogenic role of PARG in BaP induced carcinogenesis, which provided a new perspective for our understanding in BaP exposure induced cancer.
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- 2016
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15. Psychometric Properties of a Generic, Patient-Centred Palliative Care Outcome Measure of Symptom Burden for People with Progressive Long Term Neurological Conditions.
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Wei Gao, Vincent Crosby, Andrew Wilcock, Rachael Burman, Eli Silber, Nilay Hepgul, K Ray Chaudhuri, Irene J Higginson, and OPTCARE Neuro trial
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Medicine ,Science - Abstract
BackgroundThere is no standard palliative care outcome measure for people with progressive long term neurological conditions (LTNC). This study aims to determine the psychometric properties of a new 8-item palliative care outcome scale of symptom burden (IPOS Neuro-S8) in this population.Data and methodsData were merged from a Phase II palliative care intervention study in multiple sclerosis (MS) and a longitudinal observational study in idiopathic Parkinson's disease (IPD), multiple system atrophy (MSA) and progressive supranuclear palsy (PSP). The IPOS Neuro-S8 was assessed for its data quality, score distribution, ceiling and floor effects, reliability, factor structure, convergent and discriminant validity, concurrent validity with generic (Palliative care Outcome Scale) and condition specific measures (Multiple Sclerosis Impact Scale; Non-motor Symptoms Questionnaire; Parkinson's Disease Questionnaire), responsiveness and minimally clinically important difference.ResultsOf the 134 participants, MS patients had a mean Extended Disability Status Scale score 7.8 (SD = 1.0), patients with an IPD, MSA or PSP were in Hoehn & Yahr stage 3-5. The IPOS Neuro-S8 had high data quality (2% missing), mean score 8 (SD = 5; range 0-32), no ceiling effects, borderline floor effects, good internal consistency (Cronbach's α = 0.7) and moderate test-retest reliability (intraclass coefficient = 0.6). The results supported a moderately correlated two-factor structure (Pearson's r = 0.5). It was moderately correlated with generic and condition specific measures (Pearson's r: 0.5-0.6). There was some evidence for discriminant validity in IPD, MSA and PSP (p = 0.020), and for good responsiveness and longitudinal construct validity.ConclusionsIPOS Neuro-S8 shows acceptable to promising psychometric properties in common forms of progressive LTNCs. Future work needs to confirm these findings with larger samples and its usefulness in wider disease groups.
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- 2016
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16. Association between Time of Day of Sports-Related Physical Activity and the Onset of Acute Myocardial Infarction in a Chinese Population.
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Shan Zhao, Zhen Zhang, Qingqing Long, Yao Ma, Xiaoqing Lian, Yang Yang, Wei Gao, Zhong Chen, and Liansheng Wang
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Medicine ,Science - Abstract
OBJECTIVE:To investigate the association between the time of day of sports-related physical activity and the onset of acute myocardial infarction (AMI) in a coronary artery disease (CAD) population in China. METHODS:Between February 2014 and March 2015, a total of 696 patients from Nanjing, China, who had CAD were studied and divided into two groups (Non-AMI and AMI groups). The work-related activity and sports-related physical activity information were obtained from a self-reporting predesigned patient questionnaire. RESULTS:Sports-related physical activity was associated with a lower risk of the onset of AMI, after adjusting the established and potential confounders, with an adjusted odds ratio (OR) of 0.67 (95% CI, 0.47-0.94) compared with those who did not have any sports-related physical activity. A dose-response relationship was observed for intensity, duration, and frequency of sports-related physical activity. Further stratification analysis revealed that the protective effects of sports-related physical activity were significant in the morning and evening groups, and patients who exercised in the evening were at a lower risk of AMI than those doing sports-related physical activity in the morning. The adjusted ORs for doing sports-related physical activity in the morning and evening groups were 0.60(0.36-0.98) and 0.56(0.37-0.87), respectively, compared with inactivity (all P
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- 2016
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17. A Nomogram to Predict Adequate Lymph Node Recovery before Resection of Colorectal Cancer.
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Zhen-Yu Zhang, Cong Li, Wei Gao, Xiao-Wei Yin, Qi-Feng Luo, Nan Liu, Shiva Basnet, Zhen-Ling Dai, and Hai-Yan Ge
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Medicine ,Science - Abstract
Increased lymph node count (LNC) has been associated with prolonged survival in colorectal cancer (CRC), but the underlying mechanisms are still poorly understood. The study aims to identify new predictors and develop a preoperative nomogram for predicting the probability of adequate LNC (≥ 12). 501 eligible patients were retrospectively selected to identify clinical-pathological factors associated with LNC ≥ 12 through univariate and multivariate logistic regression analyses. The nomogram was built according to multivariate analyses of preoperative factors. Model performance was assessed with concordance index (c-index) and area under the receiver operating characteristic curve (AUC), followed by internal validation and calibration using 1000-resample bootstrapping. Clinical validity of the nomogram and LNC impact on stage migration were also evaluated. Multivariate analyses showed patient age, CA19-9, circulating lymphocytes, neutrophils, platelets, tumor diameter, histology and deposit significantly correlated with LNC (P < 0.05). The effects were marginal for CEA, anemia and CRC location (0.05 < P < 0.1). The multivariate analyses of preoperative factors suggested decreased age, CEA, CA19-9, neutrophils, proximal location, and increased platelets and diameter were significantly associated with increased probability of LNC ≥ 12 (P < 0.05). The nomogram achieved c-indexes of 0.75 and 0.73 before and after correction for overfitting. The AUC was 0.75 (95% CI, 0.70-0.79) and the clinically valid threshold probabilities were between 10% and 60% for the nomogram to predict LNC < 12. Additionally, increased probability of adequate LNC before surgery was associated with increased LNC and negative lymph nodes rather than increased positive lymph nodes, lymph node ratio, pN stages or AJCC stages. Collectively, the results indicate the LNC is multifactorial and irrelevant to stage migration. The significant correlations with preoperative circulating markers may provide new explanations for LNC-related survival advantage which is reflected by the implication of regional and systemic antitumor immune responses.
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- 2016
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18. miR-125b Enhances IL-8 Production in Early-Onset Severe Preeclampsia by Targeting Sphingosine-1-Phosphate Lyase 1.
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Weiwei Yang, Anning Wang, Chunling Zhao, Qinghua Li, Zhifang Pan, Xuefu Han, Cuijuan Zhang, Guohui Wang, Chao Ji, Guili Wang, Guangtao Jia, Jiyu Ju, Wei Gao, Wenjing Yu, Xiaoying Liu, Xi Chen, Weiguo Feng, Zhiqin Gao, Jie Li, and Chune Ren
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Medicine ,Science - Abstract
Preeclampsia (PE) is one of the leading causes of maternal and perinatal mortality and morbidity. One of the main hallmarks observed in PE is impaired inflammation state. In the current study, we found that miR-125b was deregulated in placental tissues and plasma derived from PE patients, which suggest a potential association between this miRNA and the pathogenesis of PE. Overexpression of miR-125b significantly reduced SGPL1 expression, and luciferase assays confirmed that SGPL1 is a direct target of miR-125b. We also found that miR-125b enhanced IL-8 production by directly targeting sphingosine-1-phosphate lyase 1 (SGPL1), and this effect could be reversed by SGPL1 overexpression. In placentas derived from PE patients, a negative correlation of miR-125b and SGPL1 was observed, and IL-8 was validated to be increased in the circulation of PE patients. Our data demonstrated a critical role of miR-125b in IL-8 production and the development of PE.
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- 2016
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19. Measuring Hair Cortisol Concentrations to Assess the Effect of Anthropogenic Impacts on Wild Chimpanzees (Pan troglodytes).
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Esther H D Carlitz, Robert Miller, Clemens Kirschbaum, Wei Gao, Daniel C Hänni, and Carel P van Schaik
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Medicine ,Science - Abstract
Non-human primates face major environmental changes due to increased human impacts all over the world. Although some species are able to survive in certain landscapes with anthropogenic impact, their long-term viability and fitness may be decreased due to chronic stress. Here we assessed long-term stress levels through cortisol analysis in chimpanzee hair obtained from sleeping nests in northwestern Uganda, in order to estimate welfare in the context of ecotourism, forest fragmentation with human-wildlife conflicts, and illegal logging with hunting activity (albeit not of primates), compared with a control without human contact or conflict. Concerning methodological issues, season [F(2,129) = 37.4, p < 0.0001, r2 = 0.18] and the age of nests [F(2,178) = 20.3, p < 0.0001, r2 = 0.11] significantly predicted hair cortisol concentrations (HCC). With regard to effects of anthropogenic impacts, our results neither showed elevation of HCC due to ecotourism, nor due to illegal logging compared to their control groups. We did, however, find significantly increased HCC in the fragment group compared to chimpanzees living in a nearby intact forest [F(1,88) = 5.0, p = 0.03, r2 = 0.20]. In conclusion, our results suggest that hair cortisol analysis is a powerful tool that can help understanding the impact of anthropogenic disturbances on chimpanzee well-being and could be applied to other great ape species.
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- 2016
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20. Plasma Catestatin: A Useful Biomarker for Coronary Collateral Development with Chronic Myocardial Ischemia.
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Weixian Xu, Haiyi Yu, Weihong Li, Wei Gao, Lijun Guo, and Guisong Wang
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Medicine ,Science - Abstract
BACKGROUNDS:Catestatin is an endogenous multifunctional neuroendocrinepeptide. Recently, catestatin was discovered as a novel angiogenic cytokine. The study was to investigate the associations between endogenous catestatin and coronary collateral development among the patients with chronic myocardial ischemia. METHODS:Thirty-eight patients with coronary artery chronic total occlusions (CTO) (CTO group) and 38 patients with normal coronary arteries (normal group) were enrolled in the series. Among the patients with CTO, coronary collateral development was graded according to the Rentrop score method. Rentrop score 0-1 collateral development was regarded as poor collateral group and 2-3 collateral development was regarded as good collateral group. Plasma catestatin level and vascular endothelial growth factor (VEGF) were measured by ELISA kits. RESULTS:The plasma catestatin levels in CTO group were significantly higher than that in normal group (1.97±1.01 vs 1.36±0.97ng/ml, p = 0.009). In the CTO group, the patients with good collateral development had significantly higher catestatin and VEGF levels than those with poor collateral development (2.36±0.73 vs 1.61±1.12 ng/ml, p = 0.018; 425.23±140.10 vs 238.48±101.00pg/mL, p
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- 2016
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21. Investigating Tissue Optical Properties and Texture Descriptors of the Retina in Patients with Multiple Sclerosis.
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Boglárka Enikő Varga, Wei Gao, Kornélia Lenke Laurik, Erika Tátrai, Magdolna Simó, Gábor Márk Somfai, and Delia Cabrera DeBuc
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Medicine ,Science - Abstract
PurposeTo assess the differences in texture descriptors and optical properties of retinal tissue layers in patients with multiple sclerosis (MS) and to evaluate their usefulness in the detection of neurodegenerative changes using optical coherence tomography (OCT) image segmentation.Patients and methods38 patients with MS were examined using Stratus OCT. The raw macular OCT data were exported and processed using OCTRIMA software. The enrolled eyes were divided into two groups, based on the presence of optic neuritis (ON) in the history (MSON+ group, n = 36 and MSON- group, n = 31). Data of 29 eyes of 24 healthy subjects (H) were used as controls. A total of seven intraretinal layers were segmented and thickness as well as optical parameters such as contrast, fractal dimension, layer index and total reflectance were measured. Mixed-model ANOVA analysis was used for statistical comparisons.ResultsSignificant thinning of the retinal nerve fiber layer (RNFL), ganglion cell/inner plexiform layer complex (GCL+IPL) and ganglion cell complex (GCC, RNFL+GCL+IPL) was observed between study groups in all comparisons. Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups. Higher fractal dimension values were observed in GCL+IPL and INL layers when comparing H vs. MSON+ groups. A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons. A significant difference was observed in total reflectance in the RNFL, GCL+IPL and GCC layers between the three examination groups.ConclusionTexture and optical properties of the retinal tissue undergo pronounced changes in MS even without optic neuritis. Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.
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- 2015
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22. Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii.
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Yu-Jun Zhao, Xin Chen, Meng Zhang, Ping Su, Yu-Jia Liu, Yu-Ru Tong, Xiu-Juan Wang, Lu-Qi Huang, and Wei Gao
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Medicine ,Science - Abstract
Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.
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- 2015
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23. The Value of BISAP Score for Predicting Mortality and Severity in Acute Pancreatitis: A Systematic Review and Meta-Analysis.
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Wei Gao, Hong-Xia Yang, and Cheng-En Ma
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Medicine ,Science - Abstract
The Bedside Index for Severity in Acute Pancreatitis (BISAP) score has been developed to identify patients at high risk for mortality or severe disease early during the course of acute pancreatitis. We aimed to undertake a meta-analysis to quantify the accuracy of BISAP score for predicting mortality and severe acute pancreatitis (SAP).We searched the databases of Pubmed, Embase, and the Cochrane Library to identify studies using the BISAP score to predict mortality or SAP. The pooled sensitivity, specificity, likelihood ratios, and diagnostic odds ratio (DOR) were calculated from each study and were compared with the traditional scoring systems.Twelve cohorts from 10 studies were included. The overall sensitivity of a BISAP score of ≥3 for mortality was 56% (95% CI, 53%-60%), with a specificity of 91% (95% CI, 90%-91%). The positive and negative likelihood ratios were 5.65 (95% CI, 4.23-7.55) and 0.48 (95% CI, 0.41-0.56), respectively. Regarding the outcome of SAP, the pooled sensitivity was 51% (43%-60%), and the specificity was 91% (89%-92%). The pooled positive and negative likelihood ratios were 7.23 (4.21-12.42) and 0.56 (0.44-0.71), respectively. Compared with BISAP score, the Ranson criteria and APACHEⅡscore showed higher sensitivity and lower specificity for both outcomes.The BISAP score was a reliable tool to identify AP patients at high risk for unfavorable outcomes. Compared with the Ranson criteria and APACHEⅡscore, BISAP score outperformed in specificity, but having a suboptimal sensitivity for mortality as well as SAP.
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- 2015
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24. Correction: The Value of BISAP Score for Predicting Mortality and Severity in Acute Pancreatitis: A Systematic Review and Meta-Analysis.
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Wei Gao, Hong-Xia Yang, and Cheng-En Ma
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Medicine ,Science - Published
- 2015
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25. The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage.
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Wei Hou, Wei Gao, Datao Wang, Qingxiu Liu, Siwen Zheng, and Yingping Wang
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Medicine ,Science - Abstract
Schisandra chinensis is a traditional Chinese medicine that has multiple biological activities, including antioxidant, anticancer, tonic, and anti-aging effects. Deoxyschisandrin (SA) and schisandrin B (SB), the two major lignans isolated from S. chinensis, exert high antioxidant activities in vitro and in vivo by scavenging free radicals, such as reactive oxygen species (ROS). Ultraviolet B-ray (UVB) radiation induces the production of ROS and DNA damage, which eventually leads to cell death by apoptosis. However, it is unknown whether SA or SB protects cells against UVB-induced cellular DNA damage. Our study showed that both SA and SB effectively protected HaCaT cells from UVB-induced cell death by antagonizing UVB-mediated production of ROS and induction of DNA damage. Our results showed that both SA and SB significantly prevented UVB-induced loss of cell viability using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays showed that the production of ROS following UVB exposure was inhibited by treatment with SA and SB. Moreover, SA and SB decreased the UVB-induced DNA damage in HaCaT cells by comet assays. In addition, SA and SB also prevented UVB-induced cell apoptosis and the cleavage of caspase-3, caspase-8 and caspase-9. In a word, our results imply that the antioxidants SA and SB could protect cells from UVB-induced cell damage via scavenging ROS.
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- 2015
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26. Correlation of plasma catestatin level and the prognosis of patients with acute myocardial infarction.
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Dan Zhu, Hong Xie, Xinyu Wang, Ying Liang, Haiyi Yu, and Wei Gao
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Medicine ,Science - Abstract
Catestatin is a peptide which is a potent inhibitor of catecholamine secretion and played essential functions in the cardiovascular system. Previous research found that dramatic changes of catestatin were associated with hemodynamics in acute myocardial infarction (AMI) during the first week after the AMI symptoms onset, but whether catestatin is also involved in the pathophysiological progression after AMI and then a predictor for outcomes is not clear. The aim of this study is to determine the correlation of plasma catestatin levels at different time points and the prognosis of AMI. 100 participants recruited were all patients with AMI, all of who received successful primary percutaneous coronary intervention (PCI) within 12h from the AMI symptom onset in our center; the concentrations of plasma catestatin were evaluated from blood samples of those 100 participants. Subsequent 65 months' follow-up was performed after discharging to evaluate cardiac adverse events and the association between catestatin levels and prognosis of AMI was examined. We confirmed the dramatic change of catestatin concentrations in the first week of AMI, and the levels of catestatin on D3 were much higher in adverse events group than those in non-adverse events group (p
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- 2015
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27. Cellular and Subcellular Immunohistochemical Localization and Quantification of Cadmium Ions in Wheat (Triticum aestivum).
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Wei Gao, Tiegui Nan, Guiyu Tan, Hongwei Zhao, Weiming Tan, Fanyun Meng, Zhaohu Li, Qing X Li, and Baomin Wang
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Medicine ,Science - Abstract
The distribution of metallic ions in plant tissues is associated with their toxicity and is important for understanding mechanisms of toxicity tolerance. A quantitative histochemical method can help advance knowledge of cellular and subcellular localization and distribution of heavy metals in plant tissues. An immunohistochemical (IHC) imaging method for cadmium ions (Cd2+) was developed for the first time for the wheat Triticum aestivum grown in Cd2+-fortified soils. Also, 1-(4-Isothiocyanobenzyl)-ethylenediamine-N,N,N,N-tetraacetic acid (ITCB-EDTA) was used to chelate the mobile Cd2+. The ITCB-EDTA/Cd2+ complex was fixed with proteins in situ via the isothiocyano group. A new Cd2+-EDTA specific monoclonal antibody, 4F3B6D9A1, was used to locate the Cd2+-EDTA protein complex. After staining, the fluorescence intensities of sections of Cd2+-positive roots were compared with those of Cd2+-negative roots under a laser confocal scanning microscope, and the location of colloidal gold particles was determined with a transmission electron microscope. The results enable quantification of the Cd2+ content in plant tissues and illustrate Cd2+ translocation and cellular and subcellular responses of T. aestivum to Cd2+ stress. Compared to the conventional metal-S coprecipitation histochemical method, this new IHC method is quantitative, more specific and has less background interference. The subcellular location of Cd2+ was also confirmed with energy-dispersive X-ray microanalysis. The IHC method is suitable for locating and quantifying Cd2+ in plant tissues and can be extended to other heavy metallic ions.
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- 2015
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28. Human Monoclonal Antibody Targeting the Heparan Sulfate Chains of Glypican-3 Inhibits HGF-Mediated Migration and Motility of Hepatocellular Carcinoma Cells.
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Wei Gao, Heungnam Kim, and Mitchell Ho
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Medicine ,Science - Abstract
Heparan sulfate proteoglycans (HSPGs) participate in many processes related to tumor development, including tumorigenesis and metastasis. HSPGs contain one or more heparan sulfate (HS) chains that are covalently linked to a core protein. Glypican-3 (GPC3) is a cell surface-associated HSPG that is highly expressed in hepatocellular carcinoma (HCC). GPC3 is involved in Wnt3a-dependent HCC cell proliferation. Our previous study reported that HS20, a human monoclonal antibody targeting the HS chains on GPC3, inhibited Wnt3a/β-catenin activation. In the current study, we showed that the HS chains of GPC3 could mediate HCC cells' migration and motility. Knocking down GPC3 or targeting the HS chains by HS20 inhibited HCC cell migration and motility. However, HS20 had no effect on GPC3 knockdown cells or GPC3 negative cells. In addition, an antibody that recognizes the core protein of GPC3 did not change the rate of cell motility. HCC cell migration and motility did not respond to either canonical or non-canonical Wnt induction, but did increase under hepatocyte growth factor (HGF) treatment. HS20-treated HCC cells exhibited less ability for HGF-mediated migration and motility. Furthermore, HS20 inhibited in vitro HCC spheroid formation and liver tumor growth in mice. GPC3 interacted with HGF; however, a mutant GPC3 lacking the HS chain showed less interaction with HGF. Blocking the HS chains on GPC3 with HS20 reduced c-Met activation in HGF-treated HCC cells and 3D-cultured spheroids. Taken together, our study suggests that GPC3 is involved in HCC cell migration and motility through HS chain-mediated cooperation with the HGF/Met pathway, showing how HS targeting has potential therapeutic implications for liver cancer.
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- 2015
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29. Research on the reproductive biological characteristics of Amomum villosum Lour. and Amomum longiligulare T. L. Wu
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Ruipei Yang, Jing Su, Jie Wang, Wei Gao, He Guozhen, Ye Jiang, and Dongmei Sun
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Germplasm ,Atmospheric Science ,Pollination ,Physiology ,Science ,Flowers ,Plant Science ,Biology ,medicine.disease_cause ,Amomum ,Fruits ,Meteorology ,Pollen ,medicine ,Flower Anatomy ,Flowering Plants ,Multidisciplinary ,Phenology ,Pollination management ,Reproduction ,Plant Anatomy ,Organisms ,Amomum villosum ,Biology and Life Sciences ,Eukaryota ,Agriculture ,Humidity ,Plants ,biology.organism_classification ,Agronomy ,Horticulture ,Pistils ,Stigma ,Plant Breeding ,Germination ,Fruit ,Plant Physiology ,Earth Sciences ,Medicine ,Research Article - Abstract
Objective To explore the influence of biological characteristics on the yield of Amomum villosum Lour. and Amomum longiligulare T. L. Wu, to find an effective pollen viability evaluation method and storage method to solve the problem of the low yield of Amomum plants. Methods Five germplasm of Amomum plants were used to investigate the effects of the phenological phase, pollen viability, and stigma receptivity on natural and artificial fruit set. Results Amomum longiligulare T. L. Wu showed late flowering, and its natural pollination rate is higher than that of Amomum villosum Lour. In all germplasm, the artificial pollination rate and fruit setting rate are more than 3 times higher than that under natural conditions. Fruits begin to drop seven days after successful pollination, and the fruit drop is basically stable after one month. The hybridization verification showed that TTC method was simpler and more accurate than in vitro germination method. Optimal storage conditions for pollen are 4°C and high humidity. After 36 h of storage, pollen can still be used for artificial cross-pollination or as hybrid parents. Conclusion The special biological characteristics are the fundamental reason for the low natural pollination rate of Amomum plants. The accurate measurement method of Amomum plants pollen is the TTC method, and storage at 4°C and high humidity can increase the yield, which was six times that of the natural yield.
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- 2020
30. A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization
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Cai-Yun Liu, Zhixiang Lv, Hong-Tao Zhu, Xiao-Man Fan, Ye-Wang Zhang, and Li-Wei Gao
- Subjects
0106 biological sciences ,0301 basic medicine ,Models, Molecular ,Protein Conformation ,01 natural sciences ,Physical Chemistry ,Biochemistry ,Medicine and Health Sciences ,Bacteroides ,Magnesium ,Recombinant Protein Purification ,Cloning, Molecular ,chemistry.chemical_classification ,Multidisciplinary ,Crystallography ,Sulfates ,Physics ,Drugs ,Heparin ,Condensed Matter Physics ,Recombinant Proteins ,Enzymes ,Molecular Docking Simulation ,Chemistry ,Physical Sciences ,Crystal Structure ,Medicine ,medicine.drug ,Protein Binding ,Research Article ,Protein Purification ,Science ,Research and Analysis Methods ,03 medical and health sciences ,Enzyme activator ,Affinity chromatography ,Bacterial Proteins ,010608 biotechnology ,medicine ,Solid State Physics ,Binding site ,Pharmacology ,Manganese ,Binding Sites ,Molecular mass ,Chemical Bonding ,Bacteria ,Gut Bacteria ,Organisms ,Chemical Compounds ,Substrate (chemistry) ,Biology and Life Sciences ,Proteins ,Hydrogen Bonding ,Enzyme Activation ,030104 developmental biology ,Isoelectric point ,Enzyme ,chemistry ,Heparin Lyase ,Enzymology ,Calcium ,Salts ,Purification Techniques - Abstract
As one of the most extensively studied glycosaminoglycan lyases, heparinase I has been used in producing low or ultra-low molecular weight heparin. Its' important applications are to neutralize the heparin in human blood and analyze heparin structure in the clinic. However, the low productivity and activity of the enzyme have greatly hindered its applications. In this study, a novel Hep-I from Bacteroides cellulosilyticus (BcHep-I) was successfully cloned and heterologously expressed in E. coli BL21 (DE3) as a soluble protein. The molecular mass and isoelectric point (pI) of the enzyme are 44.42 kDa and 9.02, respectively. And the characterization of BcHep-I after purified with Ni-NTA affinity chromatography suggested that it is a mesophilic enzyme. BcHep-I can be activated by 1 mM Ca2+, Mg2+, and Mn2+, while severely inhibited by Zn2+, Co2+, and EDTA. The specific activity of the enzyme was 738.3 U·mg-1 which is the highest activity ever reported. The Km and Vmax were calculated as 0.17 mg·mL-1 and 740.58 U·mg-1, respectively. Besides, the half-life of 300 min at 30°C showed BcHep-I has practical applications. Homology modeling and substrate docking revealed that Gln15, Lys74, Arg76, Lys104, Arg149, Gln208, Tyr336, Tyr342, and Lys338 were mainly involved in the substrate binding of Hep-I, and 11 hydrogen bonds were formed between heparin and the enzyme. These results indicated that BcHep-I with high activity has great potential applications in the industrial production of heparin, especially in the clinic to neutralize heparin.
- Published
- 2020
31. Regional variations in geographic access to inpatient hospices and Place of death: A Population-based study in England, UK
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Chukwusa, Emeka, primary, Yu, Peihan, additional, Verne, Julia, additional, Taylor, Ros, additional, Higginson, Irene J., additional, and Wei, Gao, additional
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- 2020
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32. Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells
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Wei Gao, Jin-Yu Zhang, Xinyao Feng, Chang-Jiang Wang, Ze-Wen Yu, Jian Chen, Jin-Ping Hu, Hai-Xiang Guo, Bao Yuan, and Wen-Zhi Ren
- Subjects
Male ,0301 basic medicine ,Pituitary gland ,Physiology ,Peptide Hormones ,Gene Expression ,Apoptosis ,Nervous System ,Biochemistry ,Rats, Sprague-Dawley ,0302 clinical medicine ,Gene expression ,Medicine and Health Sciences ,3' Untranslated Regions ,Multidisciplinary ,Cell Death ,Transfection ,Growth hormone secretion ,Cell biology ,Nucleic acids ,medicine.anatomical_structure ,Cell Processes ,Pituitary Gland ,030220 oncology & carcinogenesis ,Medicine ,Anatomy ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,endocrine system ,Science ,Primary Cell Culture ,Endocrine System ,DNA construction ,Biology ,Research and Analysis Methods ,Cell Line ,03 medical and health sciences ,Anterior pituitary ,Pituitary Gland, Anterior ,Pituitary Hormones, Anterior ,microRNA ,Genetics ,medicine ,Animals ,Secretion ,RNA, Messenger ,Non-coding RNA ,Molecular Biology Techniques ,Molecular Biology ,Natural antisense transcripts ,Biology and Life Sciences ,Cell Biology ,Hormones ,Gene regulation ,Rats ,Neuroanatomy ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Cell culture ,Growth Hormone ,Plasmid Construction ,RNA ,Physiological Processes ,Neuroscience - Abstract
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
- Published
- 2019
33. Downregulation of microRNA-145 may contribute to liver fibrosis in biliary atresia by targeting ADD3
- Author
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Lei Liu, Zimin Chen, Zhihan Li, Dahao Zhang, Lihui Zhang, Qi Feng, Jian-yao Wang, Bin Wang, Xiaoshuo Ye, Zhouguang Wu, Yongqin Ye, and Wei Gao
- Subjects
0301 basic medicine ,Liver Cirrhosis ,Male ,Cirrhosis ,lcsh:Medicine ,Gene Expression ,Gastroenterology ,Biochemistry ,Liver disease ,Binding Analysis ,Gene expression ,Medicine and Health Sciences ,lcsh:Science ,Cells, Cultured ,Regulation of gene expression ,Multidisciplinary ,Liver Diseases ,Luciferase Assay ,Enzymes ,Nucleic acids ,Bioassays and Physiological Analysis ,Liver Fibrosis ,Female ,Oxidoreductases ,Luciferase ,Cell Binding Assay ,Research Article ,medicine.medical_specialty ,Down-Regulation ,Gastroenterology and Hepatology ,Biology ,Transfection ,Research and Analysis Methods ,03 medical and health sciences ,Extraction techniques ,Downregulation and upregulation ,Biliary atresia ,Biliary Atresia ,Internal medicine ,medicine ,Genetics ,Humans ,Molecular Biology Techniques ,Non-coding RNA ,Molecular Biology ,Chemical Characterization ,Enzyme Assays ,Retrospective Studies ,Three prime untranslated region ,lcsh:R ,Biology and Life Sciences ,Proteins ,Infant ,medicine.disease ,Molecular biology ,RNA extraction ,Gene regulation ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Case-Control Studies ,Hepatic stellate cell ,Enzymology ,RNA ,lcsh:Q ,Calmodulin-Binding Proteins ,Biochemical Analysis - Abstract
Background and objectives Biliary atresia (BA) is a pediatric liver disease characterized by fibro-obliteration and obstruction of the extrahepatic biliary system, that invariably leads to cirrhosis and even death, if left untreated for extended time. However, its pathology and etiology still remained unknown. In this study, we tested the expression of adducin 3 (ADD3), the gene identified as a susceptibility gene in BA by GWAS, and uncovered its upstream regulatory microRNA in the pathogenesis of BA. Methods In this study, 14 infants with BA and 14 infants with choledochal cyst (CC) were enrolled as experimental group and control group, respectively. ADD3 and microRNA-145 (miR-145) expression profiles in liver tissues of BA and CC were determined using qPCR. Luciferase reporter assay was performed to verify the direct interaction between miR-145-5p and ADD3 3’ Untranslated Regions (3’UTR). The Lentiviral vectors containing miR-145, miR-145-3p inhibitor, miR-145-5p inhibitor, empty vector were transfected into human hepatic stellate cell line (LX-2) to determine the functional effect of miR-145 on ADD3 expression at both mRNA and protein level. Results MiR-145 was shown to be down-regulated in liver tissues of infants with BA compared to CC (p = 0.0267). ADD3, verified as a target of miR-145-5p, was shown to be overexpressed in infants with BA at the mRNA level (p = 0.0118). Transfection of lentiviruses containing miR-145 into LX-2 cells decreased the expression of ADD3 at both mRNA and protein level compared to negative control group, and suppressed the expression of p-Akt at protein level. Conclusions Our study has shown that overexpressed ADD3 and downregulated miR-145 were detected in BA liver tissues. MiR-145-5p was confirmed to target ADD3 by luciferase reporter assay. The downregulation of miR-145 may contribute to liver fibrosis in BA by upregulating the expression of ADD3.
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- 2017
34. A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization.
- Author
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Li-Wei Gao, Hong-Tao Zhu, Cai-Yun Liu, Zhi-Xiang Lv, Xiao-Man Fan, and Ye-Wang Zhang
- Subjects
- *
MOLECULAR weights , *BACTEROIDES , *AFFINITY chromatography , *MOLECULAR cloning , *ISOELECTRIC point , *GLYCOSAMINOGLYCANS , *ENOXAPARIN - Abstract
As one of the most extensively studied glycosaminoglycan lyases, heparinase I has been used in producing low or ultra-low molecular weight heparin. Its' important applications are to neutralize the heparin in human blood and analyze heparin structure in the clinic. However, the low productivity and activity of the enzyme have greatly hindered its applications. In this study, a novel Hep-I from Bacteroides cellulosilyticus (BcHep-I) was successfully cloned and heterologously expressed in E. coli BL21 (DE3) as a soluble protein. The molecular mass and isoelectric point (pI) of the enzyme are 44.42 kDa and 9.02, respectively. And the characterization of BcHep-I after purified with Ni-NTA affinity chromatography suggested that it is a mesophilic enzyme. BcHep-I can be activated by 1 mM Ca2+, Mg2+, and Mn2+, while severely inhibited by Zn2+, Co2+, and EDTA. The specific activity of the enzyme was 738.3 U⋅mg-1 which is the highest activity ever reported. The Km and Vmax were calculated as 0.17 mg⋅mL-1 and 740.58 U⋅mg-1, respectively. Besides, the half-life of 300 min at 30°C showed BcHep-I has practical applications. Homology modeling and substrate docking revealed that Gln15, Lys74, Arg76, Lys104, Arg149, Gln208, Tyr336, Tyr342, and Lys338 were mainly involved in the substrate binding of Hep-I, and 11 hydrogen bonds were formed between heparin and the enzyme. These results indicated that BcHep-I with high activity has great potential applications in the industrial production of heparin, especially in the clinic to neutralize heparin. [ABSTRACT FROM AUTHOR]
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- 2020
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35. A two-stage classification method for borehole-wall images with support vector machine
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Laxmisha Rai, Zhaopeng Deng, Maoyong Cao, and Wei Gao
- Subjects
Computer and Information Sciences ,Support Vector Machine ,Imaging Techniques ,Feature vector ,Kernel Functions ,Borehole ,lcsh:Medicine ,02 engineering and technology ,Image Analysis ,010502 geochemistry & geophysics ,Research and Analysis Methods ,01 natural sciences ,Structural Geology ,Machine Learning ,Gabor filter ,Region of interest ,Artificial Intelligence ,Histogram ,Support Vector Machines ,0202 electrical engineering, electronic engineering, information engineering ,Image Processing, Computer-Assisted ,Rock mass classification ,lcsh:Science ,Operator Theory ,Artificial Neural Networks ,0105 earth and related environmental sciences ,Computational Neuroscience ,Multidisciplinary ,Contextual image classification ,business.industry ,Applied Mathematics ,Simulation and Modeling ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Pattern recognition ,Geology ,Support vector machine ,Physical Sciences ,Earth Sciences ,020201 artificial intelligence & image processing ,lcsh:Q ,Artificial intelligence ,business ,Mathematics ,Algorithms ,Research Article ,Neuroscience - Abstract
Analyzing geological drilling hole images acquired by Axial View Panoramic Borehole Televiewer (APBT) is a key step to explore the geological structure in a geological exploration. Conventionally, the borehole images are examined by technicians, which is inefficient and subjective. In this paper, three dominant types of borehole-wall images on coal-rock mass structure, namely, border images, fracture images and intact rock mass images are mainly studied. The traditional image classification methods based on unified feature extraction algorithm and single classifier is not effect for the borehole images. Therefore, this paper proposes a novel two-stage classification approach to improve the classification performance of borehole images. In the first-stage classification, the border images are identified from three kinds of images based on texture features and gray-scale histograms features. For the remaining two types of images, in the second-stage classification, Gabor filter is first applied to segment the region of interest (ROI) (such as microfracture, absciss layer and horizontal cracks, etc.) and the central interference region. Then, using the same feature vector after eliminating the central interference region, fracture images are separated from intact rock mass images. We test our two-stage classification system with real borehole images. The results of experimental show that the two-stage classification method can effectively classify three major borehole-wall images with the correction rate of 95.55% in the first stage and 95% in the second stage.
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- 2017
36. Cutaneous transcriptome analysis in NIH hairless mice
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Jian Chen, Jin-Ping Hu, Zhong-Hao Ji, Jin-Yu Zhang, Fu-Shi Quan, Wei Gao, Bao Yuan, and Wen-Zhi Ren
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0301 basic medicine ,Hair Growth ,Physiology ,Gene Identification and Analysis ,Gene Expression ,lcsh:Medicine ,Genetic Networks ,medicine.disease_cause ,Transcriptome ,Mice ,Gene expression ,Medicine and Health Sciences ,Gene Regulatory Networks ,lcsh:Science ,Immune Response ,Skin ,Multidisciplinary ,integumentary system ,Wnt signaling pathway ,Animal Models ,Cell cycle ,Class Ia Phosphatidylinositol 3-Kinase ,Phenotype ,medicine.anatomical_structure ,Experimental Organism Systems ,Female ,Anatomy ,Integumentary System ,Network Analysis ,Research Article ,Computer and Information Sciences ,Immunology ,Mouse Models ,Biology ,Research and Analysis Methods ,03 medical and health sciences ,Model Organisms ,Hair Follicles ,Genetics ,medicine ,Animals ,Mice, Hairless ,Sequence Analysis, RNA ,Gene Expression Profiling ,lcsh:R ,Biology and Life Sciences ,Hair follicle ,Molecular biology ,Hairless ,Gene expression profiling ,Gene Ontology ,030104 developmental biology ,lcsh:Q ,Physiological Processes ,Carcinogenesis ,Hair - Abstract
Mice with spontaneous coat mutations are ideal animal models for studying skin development and tumorigenesis. In this study, skin hair growth cycle abnormalities were examined in NIH hairless mice 42 days after birth (P42) by using hematoxylin-eosin (H&E) staining. To examine the gene expression patterns in the skin of mutant mice, the dorsal skin of P42 female NIH mice and NIH hairless mice was sequenced by RNA-Seq, and 5,068 differentially expressed genes (DEGs) were identified (false discovery rate [FDR] ≥ 2, P < 0.05). A pathway analysis showed that basal cell carcinoma, the cell cycle and the Hippo, Hedgehog and Wnt signaling pathways were up-regulated in NIH hairless mice. Previous studies have shown that these pathways are closely associated with cell proliferation, cell cycle, organ size and cancer development. In contrast, signal transduction, bacterial and parasitic infection, and receptor-mediated pathways, including calcium signaling, were down-regulated in NIH hairless mice. A gene interaction network analysis was performed to identify genes related to hair follicle development. To verify the reliability of the RNA-Seq results, we used q-PCR to analyze 12 key genes identified from the gene interaction network analysis, including eight down-regulated and four up-regulated genes, and the results confirmed the reliability of the RNA-Seq results. Finally, we constructed the differential gene expression profiles of mutant mice by RNA-Seq. NIH hairless mice exhibited abnormalities in hair development and immune-related pathways. Pik3r1 and Pik3r3 were identified as key genes, laying the foundation for additional in-depth studies of hairless mice.
- Published
- 2017
37. Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs (Sus scrofa)
- Author
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Huansheng Yang, Chien-An Andy Hu, Wei Gao, Xiaocheng Wang, Shengping Wang, Yulong Yin, Lijun Zou, Xia Xiong, Liping Jiang, and Min Gong
- Subjects
0301 basic medicine ,Frizzled ,Sus scrofa ,Protein Expression ,lcsh:Medicine ,Apoptosis ,Biochemistry ,Gene expression ,Intestine, Small ,Medicine and Health Sciences ,Small interfering RNAs ,Cloning, Molecular ,lcsh:Science ,Phylogeny ,Gene knockdown ,Multidisciplinary ,Cell Death ,Reverse Transcriptase Polymerase Chain Reaction ,Wnt signaling pathway ,Immunohistochemistry ,Blot ,Nucleic acids ,Jejunum ,Cell Processes ,RNA Interference ,Signal transduction ,Anatomy ,Research Article ,Cell Survival ,Colon ,Blotting, Western ,RAC1 ,Biology ,Research and Analysis Methods ,Cell Line ,03 medical and health sciences ,Ileum ,Gene Expression and Vector Techniques ,Genetics ,Animals ,Mitogen-Activated Protein Kinase 8 ,Amino Acid Sequence ,Molecular Biology Techniques ,Non-coding RNA ,Molecular Biology ,Cell Proliferation ,Molecular Biology Assays and Analysis Techniques ,030102 biochemistry & molecular biology ,Sequence Homology, Amino Acid ,Gene Expression Profiling ,lcsh:R ,Biology and Life Sciences ,Cell Biology ,Molecular biology ,Frizzled Receptors ,Gene regulation ,Gene expression profiling ,Gastrointestinal Tract ,030104 developmental biology ,Enterocytes ,RNA ,lcsh:Q ,rhoA GTP-Binding Protein ,Digestive System - Abstract
Frizzled 6 (FZD6) encodes an integral membrane protein that functions in multiple signal transduction pathways, for example, as a receptor in Wnt/planar cell polarity (PCP) signaling pathway for polarized cell migration and organ morphogenesis. Mutations in FZD6 have been identified in a variety of tumors. In this study, the full-length cDNA of Sus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that the Sfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain. Tissue distribution analysis showed that Sfz6 mRNA is ubiquitously expressed in various tissues, being highest in kidney, moderate in jejunum, ileum, colon, liver, and spleen. However, FZD6 protein is highly expressed in the heart and there was no significant difference in other tissues. The relative abundance and localization of FZD6 protein in jejunum along the crypt-villus axis was determined by Western blot and immunohistochemical localization. The results show that in the jejunum, FZD6 protein is highly expressed in the villus and less in the crypt cells. Cellular proliferation and viability assays indicate that knockdown of FZD6 with small interfering RNAs (siRNA) significantly reduced the cell viability of the intestinal porcine enterocyte cells (IPEC-J2). Furthermore, qPCR and Western blot analysis revealed that expressions of ras-related C3 botulinum toxin substrate 1 (Rac1); ras homolog gene family member A (RhoA) and c-Jun N-terminal kinase 1 (JNK1), some components of PCP signaling pathway were upregulated (P < 0.05) by knockdown of FZD6 in IPEC-J2 cells. In conclusion, these results showed that FZD6 abundance in the villus was higher than that in crypt cells and knockdown of FZD6 induces PCP signal pathway components expression in IPEC-J2 cells. Our findings provide the foundation for further investigation into porcine FZD6 gene.
- Published
- 2016
38. Poly(ADP-Ribose) Glycohydrolase (PARG) Silencing Suppresses Benzo(a)pyrene Induced Cell Transformation
- Author
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Wei Gao, Haiyan Huang, Jianjun Liu, Xuan Li, Zhiliang Zhu, Xiyi Li, Zhixiong Zhuang, Peiwu Huang, and Yinpin Liu
- Subjects
0301 basic medicine ,DNA Repair ,Carcinogenesis ,Cell ,Protozoology ,Micronuclei ,medicine.disease_cause ,Cell Transformation ,Biochemistry ,chemistry.chemical_compound ,Mice ,Medicine and Health Sciences ,polycyclic compounds ,PARG ,Mice, Inbred BALB C ,Multidisciplinary ,Chromosome Biology ,Animal Models ,Chromosomal Aberrations ,Nucleic acids ,Cell Motility ,medicine.anatomical_structure ,Cell Transformation, Neoplastic ,Benzo(a)pyrene ,Oncology ,Medicine ,Female ,Research Article ,Cell Physiology ,Glycoside Hydrolases ,DNA damage ,DNA repair ,Science ,Mice, Nude ,Mouse Models ,Cell Migration ,Biology ,Research and Analysis Methods ,Microbiology ,complex mixtures ,Chromosomes ,Genomic Instability ,Cell Line ,03 medical and health sciences ,Model Organisms ,medicine ,Genetics ,Animals ,Humans ,Gene Silencing ,Poly(ADP-ribose) glycohydrolase ,Carcinogen ,Cell Nucleus ,Biology and life sciences ,Epithelial Cells ,DNA ,Cell Biology ,Molecular biology ,030104 developmental biology ,chemistry ,Developmental Biology - Abstract
Benzo(a)pyrene (BaP) is a ubiquitously distributed environmental pollutant and known carcinogen, which can induce malignant transformation in rodent and human cells. Poly(ADP-ribose) glycohydrolase (PARG), the primary enzyme that catalyzes the degradation of poly(ADP-ribose) (PAR), has been known to play an important role in regulating DNA damage repair and maintaining genomic stability. Although PARG has been shown to be a downstream effector of BaP, the role of PARG in BaP induced carcinogenesis remains unclear. In this study, we used the PARG-deficient human bronchial epithelial cell line (shPARG) as a model to examine how PARG contributed to the carcinogenesis induced by chronic BaP exposure under various concentrations (0, 10, 20 and 40 μM). Our results showed that PARG silencing dramatically reduced DNA damages, chromosome abnormalities, and micronuclei formations in the PARG-deficient human bronchial epithelial cells compared to the control cells (16HBE cells). Meanwhile, the wound healing assay showed that PARG silencing significantly inhibited BaP-induced cell migration. Furthermore, silencing of PARG significantly reduced the volume and weight of tumors in Balb/c nude mice injected with BaP induced transformed human bronchial epithelial cells. This was the first study that reported evidences to support an oncogenic role of PARG in BaP induced carcinogenesis, which provided a new perspective for our understanding in BaP exposure induced cancer.
- Published
- 2016
39. A Nomogram to Predict Adequate Lymph Node Recovery before Resection of Colorectal Cancer
- Author
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Qi-feng Luo, Zhen-ling Dai, Zhenyu Zhang, Shiva Basnet, Nan Liu, Cong Li, Xiao-wei Yin, Wei Gao, and Hai-yan Ge
- Subjects
Oncology ,Male ,Multivariate analysis ,Colorectal cancer ,Neutrophils ,Physiology ,lcsh:Medicine ,Logistic regression ,White Blood Cells ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Animal Cells ,Medicine and Health Sciences ,Lymphocytes ,lcsh:Science ,Lymph node ,Multidisciplinary ,Hematology ,Middle Aged ,Prognosis ,Body Fluids ,medicine.anatomical_structure ,Blood ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Physical Sciences ,030211 gastroenterology & hepatology ,Female ,Lymph ,Anatomy ,Cellular Types ,Colorectal Neoplasms ,Statistics (Mathematics) ,Research Article ,Platelets ,medicine.medical_specialty ,Anemia ,Immune Cells ,Immunology ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,Lymphatic System ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,Statistical Methods ,Aged ,Probability ,Retrospective Studies ,Colorectal Cancer ,Blood Cells ,Receiver operating characteristic ,business.industry ,lcsh:R ,Biology and Life Sciences ,Cancers and Neoplasms ,Cell Biology ,Nomogram ,medicine.disease ,Blood Counts ,Nomograms ,Multivariate Analysis ,Lymph Node Excision ,lcsh:Q ,Lymph Nodes ,business ,Mathematics - Abstract
Increased lymph node count (LNC) has been associated with prolonged survival in colorectal cancer (CRC), but the underlying mechanisms are still poorly understood. The study aims to identify new predictors and develop a preoperative nomogram for predicting the probability of adequate LNC (≥ 12). 501 eligible patients were retrospectively selected to identify clinical-pathological factors associated with LNC ≥ 12 through univariate and multivariate logistic regression analyses. The nomogram was built according to multivariate analyses of preoperative factors. Model performance was assessed with concordance index (c-index) and area under the receiver operating characteristic curve (AUC), followed by internal validation and calibration using 1000-resample bootstrapping. Clinical validity of the nomogram and LNC impact on stage migration were also evaluated. Multivariate analyses showed patient age, CA19-9, circulating lymphocytes, neutrophils, platelets, tumor diameter, histology and deposit significantly correlated with LNC (P < 0.05). The effects were marginal for CEA, anemia and CRC location (0.05 < P < 0.1). The multivariate analyses of preoperative factors suggested decreased age, CEA, CA19-9, neutrophils, proximal location, and increased platelets and diameter were significantly associated with increased probability of LNC ≥ 12 (P < 0.05). The nomogram achieved c-indexes of 0.75 and 0.73 before and after correction for overfitting. The AUC was 0.75 (95% CI, 0.70-0.79) and the clinically valid threshold probabilities were between 10% and 60% for the nomogram to predict LNC < 12. Additionally, increased probability of adequate LNC before surgery was associated with increased LNC and negative lymph nodes rather than increased positive lymph nodes, lymph node ratio, pN stages or AJCC stages. Collectively, the results indicate the LNC is multifactorial and irrelevant to stage migration. The significant correlations with preoperative circulating markers may provide new explanations for LNC-related survival advantage which is reflected by the implication of regional and systemic antitumor immune responses.
- Published
- 2016
40. Measuring Hair Cortisol Concentrations to Assess the Effect of Anthropogenic Impacts on Wild Chimpanzees (Pan troglodytes)
- Author
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Clemens Kirschbaum, Daniel C. Hänni, Carel P. van Schaik, Esther H.D. Carlitz, Robert Miller, Wei Gao, and University of Zurich
- Subjects
10207 Department of Anthropology ,0106 biological sciences ,0301 basic medicine ,Hydrocortisone ,Physiology ,lcsh:Medicine ,Social Sciences ,Troglodytes ,Forests ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,Stress level ,Learning and Memory ,Endocrinology ,Medicine and Health Sciences ,Psychological stress ,Psychology ,Chronic stress ,Lipid Hormones ,lcsh:Science ,Mammals ,Multidisciplinary ,biology ,Ecology ,300 Social sciences, sociology & anthropology ,Terrestrial Environments ,Ecotourism ,Vertebrates ,Apes ,Anatomy ,Integumentary System ,Research Article ,Primates ,Conservation of Natural Resources ,Pan troglodytes ,Psychological Stress ,Context (language use) ,1100 General Agricultural and Biological Sciences ,Forest fragmentation ,010603 evolutionary biology ,Ecosystems ,03 medical and health sciences ,Human Learning ,1300 General Biochemistry, Genetics and Molecular Biology ,Mental Health and Psychiatry ,medicine ,Animals ,Learning ,Chimpanzees ,1000 Multidisciplinary ,Steroid Hormones ,Endocrine Physiology ,lcsh:R ,Ecology and Environmental Sciences ,Organisms ,Cognitive Psychology ,Biology and Life Sciences ,biology.organism_classification ,Hormones ,030104 developmental biology ,Cognitive Science ,lcsh:Q ,Hair ,Neuroscience - Abstract
Non-human primates face major environmental changes due to increased human impacts all over the world. Although some species are able to survive in certain landscapes with anthropogenic impact, their long-term viability and fitness may be decreased due to chronic stress. Here we assessed long-term stress levels through cortisol analysis in chimpanzee hair obtained from sleeping nests in northwestern Uganda, in order to estimate welfare in the context of ecotourism, forest fragmentation with human-wildlife conflicts, and illegal logging with hunting activity (albeit not of primates), compared with a control without human contact or conflict. Concerning methodological issues, season [F(2,129) = 37.4, p < 0.0001, r2 = 0.18] and the age of nests [F(2,178) = 20.3, p < 0.0001, r2 = 0.11] significantly predicted hair cortisol concentrations (HCC). With regard to effects of anthropogenic impacts, our results neither showed elevation of HCC due to ecotourism, nor due to illegal logging compared to their control groups. We did, however, find significantly increased HCC in the fragment group compared to chimpanzees living in a nearby intact forest [F(1,88) = 5.0, p = 0.03, r2 = 0.20]. In conclusion, our results suggest that hair cortisol analysis is a powerful tool that can help understanding the impact of anthropogenic disturbances on chimpanzee well-being and could be applied to other great ape species.
- Published
- 2015
41. Correlation of plasma catestatin level and the prognosis of patients with acute myocardial infarction
- Author
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Wei Gao, Haiyi Yu, Hong Xie, Ying Liang, Dan Zhu, and Xinyu Wang
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Myocardial Infarction ,Hemodynamics ,lcsh:Medicine ,Internal medicine ,Blood plasma ,medicine ,Humans ,Myocardial infarction ,cardiovascular diseases ,Adverse effect ,lcsh:Science ,Multidisciplinary ,business.industry ,lcsh:R ,Percutaneous coronary intervention ,Middle Aged ,Prognosis ,medicine.disease ,Peptide Fragments ,Endocrinology ,Blood pressure ,Acute Disease ,Conventional PCI ,Cardiology ,Chromogranin A ,Female ,lcsh:Q ,Myocardial infarction diagnosis ,business ,Research Article ,Follow-Up Studies - Abstract
Catestatin is a peptide which is a potent inhibitor of catecholamine secretion and played essential functions in the cardiovascular system. Previous research found that dramatic changes of catestatin were associated with hemodynamics in acute myocardial infarction (AMI) during the first week after the AMI symptoms onset, but whether catestatin is also involved in the pathophysiological progression after AMI and then a predictor for outcomes is not clear. The aim of this study is to determine the correlation of plasma catestatin levels at different time points and the prognosis of AMI. 100 participants recruited were all patients with AMI, all of who received successful primary percutaneous coronary intervention (PCI) within 12h from the AMI symptom onset in our center; the concentrations of plasma catestatin were evaluated from blood samples of those 100 participants. Subsequent 65 months' follow-up was performed after discharging to evaluate cardiac adverse events and the association between catestatin levels and prognosis of AMI was examined. We confirmed the dramatic change of catestatin concentrations in the first week of AMI, and the levels of catestatin on D3 were much higher in adverse events group than those in non-adverse events group (p
- Published
- 2015
42. Association between Time of Day of Sports-Related Physical Activity and the Onset of Acute Myocardial Infarction in a Chinese Population
- Author
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Yao Ma, Lian-Sheng Wang, Zhong Chen, Zhen Zhang, Xiao-Qing Lian, Yang Yang, Shan Zhao, Wei Gao, and Qing-Qing Long
- Subjects
Male ,China ,medicine.medical_specialty ,Time Factors ,Population ,Myocardial Infarction ,lcsh:Medicine ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Coronary artery disease ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Surveys and Questionnaires ,Internal medicine ,Diabetes mellitus ,Odds Ratio ,medicine ,Humans ,cardiovascular diseases ,030212 general & internal medicine ,Myocardial infarction ,Exercise physiology ,lcsh:Science ,education ,Exercise ,Aged ,education.field_of_study ,Multidisciplinary ,business.industry ,lcsh:R ,Case-control study ,Odds ratio ,Middle Aged ,medicine.disease ,Stenosis ,Case-Control Studies ,Cardiology ,lcsh:Q ,Female ,business ,human activities ,Research Article ,Sports - Abstract
OBJECTIVE:To investigate the association between the time of day of sports-related physical activity and the onset of acute myocardial infarction (AMI) in a coronary artery disease (CAD) population in China. METHODS:Between February 2014 and March 2015, a total of 696 patients from Nanjing, China, who had CAD were studied and divided into two groups (Non-AMI and AMI groups). The work-related activity and sports-related physical activity information were obtained from a self-reporting predesigned patient questionnaire. RESULTS:Sports-related physical activity was associated with a lower risk of the onset of AMI, after adjusting the established and potential confounders, with an adjusted odds ratio (OR) of 0.67 (95% CI, 0.47-0.94) compared with those who did not have any sports-related physical activity. A dose-response relationship was observed for intensity, duration, and frequency of sports-related physical activity. Further stratification analysis revealed that the protective effects of sports-related physical activity were significant in the morning and evening groups, and patients who exercised in the evening were at a lower risk of AMI than those doing sports-related physical activity in the morning. The adjusted ORs for doing sports-related physical activity in the morning and evening groups were 0.60(0.36-0.98) and 0.56(0.37-0.87), respectively, compared with inactivity (all P
- Published
- 2016
43. Investigating Tissue Optical Properties and Texture Descriptors of the Retina in Patients with Multiple Sclerosis
- Author
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Kornélia Lenke Laurik, Erika Tátrai, Wei Gao, Boglárka Varga, Gábor Márk Somfai, Delia Cabrera DeBuc, and Magdolna Simó
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Multiple Sclerosis ,genetic structures ,Science ,Nerve fiber layer ,Outer plexiform layer ,Retina ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Optical coherence tomography ,Ophthalmology ,medicine ,Humans ,Optic neuritis ,Multidisciplinary ,medicine.diagnostic_test ,Chemistry ,Retinal ,Inner plexiform layer ,medicine.disease ,eye diseases ,medicine.anatomical_structure ,Case-Control Studies ,Inner nuclear layer ,030221 ophthalmology & optometry ,Medicine ,Female ,sense organs ,Tomography, Optical Coherence ,030217 neurology & neurosurgery ,Research Article - Abstract
PurposeTo assess the differences in texture descriptors and optical properties of retinal tissue layers in patients with multiple sclerosis (MS) and to evaluate their usefulness in the detection of neurodegenerative changes using optical coherence tomography (OCT) image segmentation.Patients and methods38 patients with MS were examined using Stratus OCT. The raw macular OCT data were exported and processed using OCTRIMA software. The enrolled eyes were divided into two groups, based on the presence of optic neuritis (ON) in the history (MSON+ group, n = 36 and MSON- group, n = 31). Data of 29 eyes of 24 healthy subjects (H) were used as controls. A total of seven intraretinal layers were segmented and thickness as well as optical parameters such as contrast, fractal dimension, layer index and total reflectance were measured. Mixed-model ANOVA analysis was used for statistical comparisons.ResultsSignificant thinning of the retinal nerve fiber layer (RNFL), ganglion cell/inner plexiform layer complex (GCL+IPL) and ganglion cell complex (GCC, RNFL+GCL+IPL) was observed between study groups in all comparisons. Significant difference was found in contrast in the RNFL, GCL+IPL, GCC, inner nuclear layer (INL) and outer plexiform layer when comparing MSON+ to the other groups. Higher fractal dimension values were observed in GCL+IPL and INL layers when comparing H vs. MSON+ groups. A significant difference was found in layer index in the RNFL, GCL+IPL and GCC layers in all comparisons. A significant difference was observed in total reflectance in the RNFL, GCL+IPL and GCC layers between the three examination groups.ConclusionTexture and optical properties of the retinal tissue undergo pronounced changes in MS even without optic neuritis. Our results may help to further improve the diagnostic efficacy of OCT in MS and neurodegeneration.
- Published
- 2015
44. Cellular and Subcellular Immunohistochemical Localization and Quantification of Cadmium Ions in Wheat (Triticum aestivum)
- Author
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Hongwei Zhao, Qing X. Li, Fanyun Meng, Tiegui Nan, Wei Gao, Zhaohu Li, Guiyu Tan, Baomin Wang, and Weiming Tan
- Subjects
In situ ,Fluorescence-lifetime imaging microscopy ,Cadmium ,Microscopy, Confocal ,Multidisciplinary ,lcsh:R ,lcsh:Medicine ,food and beverages ,chemistry.chemical_element ,Biology ,Subcellular localization ,Immunohistochemistry ,Fluorescence ,Microscopy, Electron, Transmission ,chemistry ,Colloidal gold ,Botany ,Biophysics ,Fluorescence microscope ,lcsh:Q ,Chelation ,lcsh:Science ,Triticum ,Research Article - Abstract
The distribution of metallic ions in plant tissues is associated with their toxicity and is important for understanding mechanisms of toxicity tolerance. A quantitative histochemical method can help advance knowledge of cellular and subcellular localization and distribution of heavy metals in plant tissues. An immunohistochemical (IHC) imaging method for cadmium ions (Cd2+) was developed for the first time for the wheat Triticum aestivum grown in Cd2+-fortified soils. Also, 1-(4-Isothiocyanobenzyl)-ethylenediamine-N,N,N,N-tetraacetic acid (ITCB-EDTA) was used to chelate the mobile Cd2+. The ITCB-EDTA/Cd2+ complex was fixed with proteins in situ via the isothiocyano group. A new Cd2+-EDTA specific monoclonal antibody, 4F3B6D9A1, was used to locate the Cd2+-EDTA protein complex. After staining, the fluorescence intensities of sections of Cd2+-positive roots were compared with those of Cd2+-negative roots under a laser confocal scanning microscope, and the location of colloidal gold particles was determined with a transmission electron microscope. The results enable quantification of the Cd2+ content in plant tissues and illustrate Cd2+ translocation and cellular and subcellular responses of T. aestivum to Cd2+ stress. Compared to the conventional metal-S coprecipitation histochemical method, this new IHC method is quantitative, more specific and has less background interference. The subcellular location of Cd2+ was also confirmed with energy-dispersive X-ray microanalysis. The IHC method is suitable for locating and quantifying Cd2+ in plant tissues and can be extended to other heavy metallic ions.
- Published
- 2015
45. Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii
- Author
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Xiu-juan Wang, Wei Gao, Yu-Jia Liu, Xin Chen, Yu-Jun Zhao, Meng Zhang, Yuru Tong, Ping Su, and Luqi Huang
- Subjects
DNA, Complementary ,Tripterygium ,Molecular Sequence Data ,Farnesyl pyrophosphate ,lcsh:Medicine ,Cyclopentanes ,Acetates ,Molecular cloning ,Gas Chromatography-Mass Spectrometry ,Terpene ,chemistry.chemical_compound ,Biosynthesis ,Gene Expression Regulation, Plant ,Amino Acid Sequence ,Oxylipins ,Cloning, Molecular ,lcsh:Science ,Phylogeny ,Plant Proteins ,Multidisciplinary ,Methyl jasmonate ,ATP synthase ,biology ,Gene Expression Profiling ,lcsh:R ,Geranyltranstransferase ,biology.organism_classification ,Recombinant Proteins ,Terpenoid ,chemistry ,Biochemistry ,Organ Specificity ,Biocatalysis ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,lcsh:Q ,Tripterygium wilfordii ,Research Article - Abstract
Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.
- Published
- 2015
46. Identification of a Plasma Four-microRNA Panel as Potential Noninvasive Biomarker for Osteosarcoma
- Author
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Ke-wei Gao, Chen-liang Zhou, Liwen Wu, Feng Lian, and Yong Cui
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Pathology ,Adolescent ,lcsh:Medicine ,Gene Expression ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Metastasis ,Young Adult ,Surgical oncology ,Internal medicine ,Blood plasma ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Metastasis ,lcsh:Science ,Child ,Osteosarcoma ,Multidisciplinary ,business.industry ,Gene Expression Profiling ,lcsh:R ,Area under the curve ,Reproducibility of Results ,medicine.disease ,Tumor Burden ,MicroRNAs ,Circulating MicroRNA ,Treatment Outcome ,Real-time polymerase chain reaction ,Case-Control Studies ,Biomarker (medicine) ,lcsh:Q ,Female ,business ,Research Article - Abstract
Background Circulating microRNAs (miRNAs) are emerging as promising biomarkers for human cancer. Osteosarcoma is the most common human primary malignant bone tumor in children and young adults. The objective of this study was to investigate whether circulating miRNAs in plasma could be a useful biomarker for detecting osteosarcoma and monitoring tumor removal dynamics. Methods Plasma samples were obtained from 90 patients before surgery, 50 patients after one month of surgery, and 90 healthy individuals. The study was divided into three steps: First, initial screening of the profiles of circulating miRNAs in pooled plasma samples from healthy controls and pre-operative osteosarcoma patients using a TaqMan low density array (TLDA). Second, evaluation of miRNA concentration in individual plasma samples from 90 pre-operative osteosarcoma patients and 90 healthy controls by a quantitative real time PCR (qRT-PCR) assay. Third, evaluation of miRNA concentration in paired plasma samples from 50 pre- and post-operative osteosarcoma patients by qRT-PCR assay. Results Four plasma miRNAs including miR-195-5p, miR-199a-3p, miR-320a, and miR-374a-5p were significantly increased in the osteosarcoma patients. Receiver operating characteristics curve analysis of the combined populations demonstrated that the four-miRNA signature could discriminate cases from controls with an area under the curve of 0.9608 (95% CI 0.9307-0.9912). These 4 miRNAs were markedly decreased in the plasma after operation. In addition, circulating miR-195-5p and miR-199a-3p were correlated with metastasis status, while miR-199a-3p and miR-320a were correlated with histological subtype. Conclusions Our data suggest that altered levels of circulating miRNAs might have great potential to serve as novel, non-invasive biomarkers for osteosarcoma.
- Published
- 2015
47. Ethanol Negatively Regulates Hepatic Differentiation of hESC by Inhibition of the MAPK/ERK Signaling Pathway In Vitro
- Author
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Yuyou Duan, Ping Zhou, Jan A. Nolta, Benjamin Tschudy-Seney, Nataly L. Magner, Wei Gao, Mark A. Zern, Jiamei Chen, Alexander Revzin, Xiaocui Ma, and Singh, Shree Ram
- Subjects
MAPK/ERK pathway ,Cell signaling ,MAP Kinase Kinase 4 ,Cellular differentiation ,lcsh:Medicine ,Signal transduction ,Regenerative Medicine ,p38 Mitogen-Activated Protein Kinases ,Oral and gastrointestinal ,Alcohol Use and Health ,Mice ,Animal Cells ,Cyclin D1 ,lcsh:Science ,beta Catenin ,Mitogen-Activated Protein Kinase 1 ,Hepatocyte differentiation ,Mitogen-Activated Protein Kinase 3 ,Multidisciplinary ,Stem Cells ,Liver Disease ,Substance Abuse ,Wnt signaling pathway ,Signaling cascades ,Cell Differentiation ,Liver regeneration ,Activins ,3. Good health ,Cell biology ,Alcoholism ,Liver ,Quinolines ,Stem Cell Research - Nonembryonic - Non-Human ,Anatomy ,Cellular Types ,Development of treatments and therapeutic interventions ,Stem cell ,Research Article ,Signal Transduction ,MAPK signaling cascades ,General Science & Technology ,1.1 Normal biological development and functioning ,Chronic Liver Disease and Cirrhosis ,Wnt1 Protein ,Biology ,Imides ,Cell Line ,Underpinning research ,Nitriles ,Butadienes ,Animals ,Humans ,Stem Cell Research - Embryonic - Human ,Progenitor cell ,Embryonic Stem Cells ,Biology and life sciences ,5.2 Cellular and gene therapies ,Ethanol ,lcsh:R ,Feeder Cells ,Fibroblasts ,Stem Cell Research ,Gene Expression Regulation ,Hepatocytes ,lcsh:Q ,Digestive Diseases - Abstract
Background: Alcohol insult triggers complex events in the liver, promoting fibrogenic/inflammatory signals and in more advanced cases, aberrant matrix deposition. It is well accepted that the regenerative capacity of the adult liver is impaired during alcohol injury. The liver progenitor/stem cells have been shown to play an important role in liver regeneration -in response to various chronic injuries; however, the effects of alcohol on stem cell differentiation in the liver are not well understood. Methods: We employed hepatic progenitor cells derived from hESCs to study the impact of ethanol on hepatocyte differentiation by exposure of these progenitor cells to ethanol during hepatocyte differentiation. Results: We found that ethanol negatively regulated hepatic differentiation of hESC-derived hepatic progenitor cells in a dose-dependent manner. There was also a moderate cell cycle arrest at G1/S checkpoint in the ethanol treated cells, which is associated with a reduced level of cyclin D1 in these cells. Ethanol treatment specifically inhibited the activation of the ERK but not JNK nor the p38 MAP signaling pathway. At the same time, the WNT signaling pathway was also reduced in the cells exposed to ethanol. Upon evaluating the effects of the inhibitors of these two signaling pathways, we determined that the Erk inhibitor replicated the effects of ethanol on the hepatocyte differentiation and attenuated the WNT/b-catenin signaling, however, inhibitors of WNT only partially replicated the effects of ethanol on the hepatocyte differentiation. Conclusion: Our results demonstrated that ethanol negatively regulated hepatic differentiation of hESC-derived hepatic progenitors through inhibiting the MAPK/ERK signaling pathway, and subsequently attenuating the WNT signaling pathway. Thus, our finding provides a novel insight into the mechanism by which alcohol regulates cell fate selection of hESC-derived hepatic progenitor cells, and the identified pathways may provide therapeutic targets aimed at promoting liver repair and regeneration during alcoholic injury.
- Published
- 2014
48. Multivariate Longitudinal Shape Analysis of Human Lateral Ventricles during the First Twenty-Four Months of Life
- Author
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Martin Styner, Hongtu Zhu, Valerie Jewells, Shun Xu, Ying Yuan, Dinggang Shen, Beatriz Paniagua, Wei Gao, Lucile Bompard, Mihye Ahn, and Weili Lin
- Subjects
Male ,Multivariate statistics ,Pediatrics ,medicine.medical_specialty ,Clinical Research Design ,lcsh:Medicine ,Developmental and Pediatric Neurology ,Research and Analysis Methods ,Lateral ventricles ,Cerebrospinal fluid ,Lateral Ventricles ,Medicine and Health Sciences ,medicine ,Humans ,In patient ,Longitudinal Studies ,lcsh:Science ,10. No inequality ,Cerebrum ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Infant ,Magnetic resonance imaging ,Anatomy ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Neurology ,Research Design ,Ventricle ,Child, Preschool ,Multivariate Analysis ,Morphological analysis ,Female ,lcsh:Q ,business ,Research Article ,Shape analysis (digital geometry) - Abstract
Background Little is known about the temporospatial shape characteristics of human lateral ventricles (LVs) during the first two years of life. This study aimed to delineate the morphological growth characteristics of LVs during early infancy using longitudinally acquired MR images in normal healthy infants. Methods 24 healthy infants were MR imaged starting from 2 weeks old every 3 months during the first and every 6 months during the second year. Bilateral LVs were segmented and longitudinal morphological and shape analysis were conducted using longitudinal mixed effect models. Results A significant bilateral ventricular volume increase (p
- Published
- 2014
49. Association between IL-6-174G/C polymorphism and the risk of sepsis and mortality: a systematic review and meta-analysis.
- Author
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Jun-wei Gao, An-qiang Zhang, Wei Pan, Cai-li Yue, Ling Zeng, Wei Gu, and Jianxin Jiang
- Subjects
Medicine ,Science - Abstract
Recent studies have reported the association between IL-6-174G/C polymorphism and sepsis. However, the results are inconclusive and conflicting. To better understand the role of IL-6-174G/C polymorphism in sepsis, we conducted a comprehensive meta-analysis.Literature search was conducted through PubMed, Embase, Web of Knowledge databases until July 29, 2013. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using fixed- or random-effect model based on heterogeneity test in total and subgroup analyses.Twenty studies on the risk of sepsis and seven studies on sepsis mortality were included. None of the results showed evidence of a significant association between IL-6-174G/C polymorphism and sepsis risk in overall analysis or subgroup analyses based on sepsis type, ethnicity, source of control and age under any genetic model (the allele comparison, the codominant, the recessive or the dominant model). Although there was a statistically significant association between IL-6-174 G/C polymorphism and sepsis-related mortality under the recessive model, the significance did not exist after Bonferroni's correction.Current evidence does not support a direct effect of IL-6-174 G/C polymorphism on the risk of sepsis. In addition, there was no association between IL-6-174 G/C polymorphism and sepsis mortality after Bonferroni's correction. Further analyses of gene-environment interactions and more studies based on larger sample size and homogeneous sepsis patients are required.
- Published
- 2015
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